Effects of Sodium Nitroprusside and Nitric Oxide on In Vitro Maturation of Canine Oocytes

  • Park, Ji-Hoon (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Bong-Koo (Dept. of Companion Animal and Animal Resourses Science, Joongbu University) ;
  • Chung, Young-Ho (Dept. of Companion Animal and Animal Resourses Science, Joongbu University) ;
  • Rhee, Man-Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, S.K. (College of Veterinary Medicine, Chungnam National University)
  • Received : 2011.11.16
  • Accepted : 2011.11.26
  • Published : 2011.12.31

Abstract

These study was carried out to investigate the effects of the supplementation with sodium nitroprusside (SN) and nitric oxide (NO) of canine oocytes on IVM rates. Oocytes were incubated in TCM-199 supplement with at 0.03~0.10 mM SN and 0.3~1.0 mM NO for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered mineral oil and cultured in a $CO_2$ incubator (5% $CO_2$, 95% air, $38^{\circ}C$). The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03, 0.05, 0.07, 0.10 mM SN were $25.9{\pm}3.5%$, $36.4{\pm}3.2%$, $33.3{\pm}3.5%$, $28.8{\m}3.2%$, respectively. The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03~0.07 mM SN were significantly increased compare to the control ($26.0{\pm}2.2%$). The in vitro maturation rates of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.3, 0.5, 0.7, 1.0 mM NO were $28.0{\pm}4.2%$, $36.5{\pm}3.6%$, $30.0{\pm}3.8%$, $19.2{\pm}3.5%$, respectively. The in vitro maturation rate of oocytes in TCM-199 medium supplemented with 0.3 and 0.5 mM NO were significantly increased compare to the control ($26.0{\pm}2.2%$). The in vitro maturation rates of oocytes cultured for 12~48 hrs in TCM-199 medium supplement with 0.05 mM SN were $26.0{\pm}3.2%$, $28.0{\pm}3.4%$, $38.0{\pm}3.2%$, respectively. The in vitro maturation rate of oocytes cultured for 12~48 hrs in TCM-199 medium supplement with 0.5 mM NO were $22.0{\pm}3.0%$, $30.0{\pm}3.8%$, $36.0{\pm}4.2%$, respectively. These result was significantly increased compare to the control.

Keywords

References

  1. Albertini DF, Combelles CM, Benecchi E, Carabatsos MJ (2001): Cellular basis for paracrine regulation of ovarian follicle development. Reproduction 121:647-653. https://doi.org/10.1530/rep.0.1210647
  2. Bertagnolli AC, Goncalves PBD, Giometti IC, Costa LFS, Oliveira JFC, Goncalves IDV, Barreto KP, Emanuelli IP, Borges LFK (2004): Interagao entre células do cumulus de atividade da proteína quinase C em diferentes fases da maturacao nuclear de oócitos bovinos. Arq. Bras. Med Vet Zootec 56:488-496. https://doi.org/10.1590/S0102-09352004000400010
  3. Bian K, Murad F (2003): Nitric oxide (NO)-biogeneration, regulation, and relevance to human diseases. Front Biosci 8:264-278. https://doi.org/10.2741/997
  4. Bu S, Xia G, Tao Y, Lei L, Zhou B (2003): Dual effects of nitric oxide on meiotic maturation of mouse cumulus cell-enclosed oocytes in vitro. Mol Cell Endocr 207:21-30. https://doi.org/10.1016/S0303-7207(03)00213-2
  5. Gouge RC, Marshburn P, Gordon BF, Nunley W, Huet-Hudson YM (1998): Nitric oxide as a regulator of embryonic development. Biol Reprod 58:875- 879. https://doi.org/10.1095/biolreprod58.4.875
  6. Herrero MB, Gagnon C (2001): Nitric oxide: a novel mediator of sperm function, J Androl 22:349-356.
  7. Ingram AJ, James L, Cai L, Thai K, Ly H, Scholey JW (2000): NO inhibits stretch-induced mapk activity by cytoskeletal disruption. J Biol Chem 51: 40301-40306.
  8. Jablonka-Shariff A, Basuray R, Olson LM (1999): Inhibitors of nitric oxide synthase influence oocyte maturation in rats. J Soc Gynecol Invest 6:95-101. https://doi.org/10.1016/S1071-5576(98)00053-7
  9. Jablonka-Shariff A, Olson LM (2000): Nitric oxide is essential for optimal meiotic maturation of murine cumulus-oocyte complexes in vitro. Mol Reprod Dev 55:412-421. https://doi.org/10.1002/(SICI)1098-2795(200004)55:4<412::AID-MRD9>3.0.CO;2-W
  10. Kanner J, Harel S, Granit R (1991): Nitric oxide as an antioxidant. Arch Biochem Biophys 289:130-136. https://doi.org/10.1016/0003-9861(91)90452-O
  11. Kazuo SK, Naoyuki T, Michiharu HK, Keiko T, Harumi K, Dinara S, Kenichi T, Mutsuo I (2001): Requirement of nitric oxide for murine oocyte maturation, embryo development, and trophoblast outgrowth in vitro. Mol Reprod Dev 58:262-268. https://doi.org/10.1002/1098-2795(200103)58:3<262::AID-MRD3>3.0.CO;2-8
  12. Kuo PC, Abe KY, Schroeder EA (1996): Interleukin- 1-induced nitric oxide production modulates gluta thione synthesis in cultured rat hepatocytes. Am J Physiol 271:851- 862. https://doi.org/10.1152/ajpcell.1996.271.3.C851
  13. Lamas S, Marsden PA, Li GK, Tempst P, Michel T (1992): Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform. Proc Natl Acad Sci 89:6348- 6352. https://doi.org/10.1073/pnas.89.14.6348
  14. Matta SGC, Bussiere MCC, Viana KS, Quirino CR (2002): Efeito de diferentes concentracoes do inibidor da sintese de oxido nitrico na maturacao nuclear in vitro de oocitos bovinos. Rev Bras Reprod Anim 26:149-151.
  15. Mehta JL, Chen LY, Kone BC, Mehta P, Turner P (1995): Identification of constitutive and inducible forms of nitric oxide synthase in human platelets. J Lab Clin Med 125:370-377.
  16. Messmer UK, Lapetina EG, Brune B (1995): Nitric oxide-induced apoptosis in raw 264.7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds. Mol Pharmacol 47:757- 765.
  17. Moncada S, Palmer RMJ, Higgs EA (1991): Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43:109-142.
  18. Nathan C (1992): Nitric oxide as a secretory product of mammalian cells. Faseb J 6:3051-3064. https://doi.org/10.1096/fasebj.6.12.1381691
  19. Olson LM, Jablonka-Shariff A, Beltsos AN (1999): Ovarian nitric oxide: a modulator of ovulation and oocyte maturation. In: F.Y. Adashi, Editor, Ovulation: Envolving Scientific and Clinical Concepts, Springer- Verlag Inc New York pp 243-264.
  20. Sengoku K, Takuma N, Horikawa M, Tsuchiya K, Komori H, Sharifa D, Tamate K, Ishikawa M (2001): Requirement of nitric oxide for murine oocyte maturation, embryo development, and trophoblast outgrowth in vitro. Mol Reprod Dev 58:252-258.
  21. Tornell J, Billig H, Hillensjo T (1990): Resumption of rat oocyte meiosis is paralleled by a decrease in guanine3',5'-cyclic monophosphate(cGMP): and is inhibited by microinjection of cGMP. Acta Physiool Scand 139:511-517. https://doi.org/10.1111/j.1748-1716.1990.tb08953.x
  22. Viana KS, Caldas-Bussiere MC, Matta SG, Faes MR, de Carvalho CS, Quirino CR (2006): Effect of sodium nitroprusside, a nitric oxide donor, on the in vitro maturation of bovine oocytes. Anim Reprod Sci 102(3-4):217-27.
  23. Webb RJ, Marshall F, Swann K, Carroll J (2002): Follicle-stimulating hormone induces a gap junctiondependent dynamic change in [camp] and protein kinase A in mammalian oocytes. Dev Biol 246:441- 454. https://doi.org/10.1006/dbio.2002.0630
  24. Xie QW, Cho HJ, Calaycay J, Mumford RA, Swiderek KM, Lee TD, Ding A, Troso T, Nathan C (1992): Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256: 225-228. https://doi.org/10.1126/science.1373522