Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Induced Pluripotent Stem Cell Generation using Nonviral Vector

  • Park, Si-Jun (School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Shin, Mi-Jung (School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Seo, Byoung-Boo (Dept. of Animal Resources, College of Life & Environmental Science, Daegu University) ;
  • Park, Hum-Dai (Dept. of Biotechnology, College of Engineering, Daegu University) ;
  • Yoon, Du-Hak (School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Ryoo, Zae-Young (School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2011.11.10
  • Accepted : 2011.11.20
  • Published : 2011.12.31
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Abstract

Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by etopic expression of transcription factors. iPS cells are indistinguishable from ES cells in terms of morphology and stem cell marker expression. Moreover, mouse iPS cells give rise to chimeric mice that are competent for germline transmission. However, mice derived from iPS cells often develop tumors. Furthermore, the low efficiency of iPS cell generation is a big disadvantage for mechanistic studies. Nonviral plasmid.based vectors are free of many of the drawbacks that constrain viral vectors. The histone deacetylase inhibitor valproic acid (VPA) has been shown to improve the efficiency of mouse and human iPS cell generation, and vitamin C (Vc) accelerates gene expression changes and establishment of the fully reprogrammed state. The MEK inhibitor PD0325901 (Stemgent) has been shown to increase the efficiency of the reprogramming of human primary fibroblasts into iPS cells. In this report, we described the generation of mouse iPS cells devoid of exogenous DNA by the simple transient transfection of a nonviral vector carrying 2A-peptide-linked reprogramming factors. We used VPA, Vc, and the MEK inhibitor PD0325901 to increase the reprogramming efficiency. The reprogrammed somatic cells expressed pluripotency markers and formed EBs.

Keywords

References

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