한국패류학회지 (The Korean Journal of Malacology)

한국패류학회 (The Malacological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

저수온 스트레스에 의한 북방전복, Haliotis discus hannai의 생리학적 반응

Physiological responses on Low Water-temperature Stress of Pacific abalone, Haliotis discus hannai

  • 박철지 (국립수산과학원 육종연구센터) ;
  • 민병화 (국립수산과학원 동해수산연구소) ;
  • 김관석 (국립수산과학원 육종연구센터) ;
  • 이장욱 (국립수산과학원 육종연구센터) ;
  • 이정호 (국립수산과학원 육종연구센터) ;
  • 노재구 (국립수산과학원 육종연구센터) ;
  • 김현철 (국립수산과학원 육종연구센터) ;
  • 박종원 (국립수산과학원 육종연구센터) ;
  • 명정인 (국립수산과학원 육종연구센터)
  • 투고 : 2011.10.21
  • 심사 : 2011.12.23
  • 발행 : 2011.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 북방전복 (H. discus hannai) 의 겨울철 저수온으로 발생하는 폐사원인 규명을 위한 생리학적인 반응을 조사하기 위하여 수온 $12^{\circ}C$를 대조구로 $7^{\circ}C$ 및 $4^{\circ}C$의 실험구를 설정하고 10일간에 걸쳐 생존율, 항산화효소의 활성 및 총 단백질 농도를 측정하였다. 그 결과 $7^{\circ}C$ 저수온 실험구에서는 SOD 활성 및 총 단백질 농도가 대조구와 차이를 나타내지 않았으며 생존율은 90.8%를 나타내었다. 반면에 $4^{\circ}C$ 저수온 실험구에서는 SOD 활성은 노출직후에 급격히 상승하여 12시간째까지 대조구보다 유의적으로 높게 나타났다. 또한 총 단백질 농도에 있어서는 24시간째부터 실험종료 시까지 대조구보다 유의적으로 높게 나타났으며, 생존율은 3일째 10마리의 폐사를 시작으로 10일째 전 개체가 페사하였다. 이상의 결과로 북방전복 (H. discus hannai) 은 $7^{\circ}C$의 저수온 실험구에서는 다양한 생리적 기작을 이용하여 생존이 가능한 반면에 $4^{\circ}C$의 저수온 실험구에서는 생리적 순응반응을 나타내고 있으나 방어기작의 한계를 벗어나 생존이 불가능하다고 추정된다.

This study was performed to obtain the basic data on physiological responses of low water temperature stress of the cultured Pacific abalone, Haliotis discus hannai. Abalones were exposed at low water temperatures of $7^{\circ}C$ and $4^{\circ}C$. We have investigated survival rate, superoxide dismutase (SOD) activity and total protein (TP) in the abalone by the exposure times (0, 3, 6, 12, 24, 72, 120, 168 and 216 hours). Survival rate of the abalone at $7^{\circ}C$ experiment was 90.8%, whereas at $4^{\circ}C$ experiment was 0% after exposure 10 days. SOD activity was significantly increased until 12 hours after exposure to $4^{\circ}C$, and then was recovered to starting level after 24 hours. However, there was no significant difference between control ($12^{\circ}C$) and $7^{\circ}C$ experiments. TP was significantly increased until 216 hours after 24 hours at $4^{\circ}C$ experiment, but $7^{\circ}C$ experiment showed no significant differences compared to control ($12^{\circ}C$) experiment. Therefore, H. discus hannai was acclimated in low water temperature stress at $7^{\circ}C$, but at $4^{\circ}C$, all abalone died possibly because they exceed the limits of defense ability to too low temperature.

키워드

참고문헌

  1. Barton, B.A. and Iwama, G.K. (1991) Physiological changes in fish from stress in aquaculture with emphasis in the response and effects of corticosteroids. Annual Review of Fish Disease, 1: 3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  2. Chance, B., Sice, H. and Boveris, A. (1979) Hydroperoxide metabolism in mammalian organs. Physiologicla Reviews, 59: 527-605.
  3. Cheng, W., Yeh, S.P., Wang C.S. and Chen J.C. (2002) Osmotic and ionic changes in Taiwan abalone Haliotis diversicolor supertexta at different salinity leverls. Aquaculture, 203: 349-357. https://doi.org/10.1016/S0044-8486(01)00606-8
  4. Davidson, G.W., Davie, P.S., Young, G. and Fowler, R.T. (2000) Physiological responses of rainbow trout Oncorhynchus mykiss to crowding and anesthesia with AQUI-STM. Jounal of World Aquaculture Society, 31: 105-114. https://doi.org/10.1111/j.1749-7345.2000.tb00704.x
  5. Ferraris, M., Radice, S., Catalani, P., Francolini, M., Marabini, L. and Chiesara, E. (2002) Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquatic Toxicology, 59: 283-296. https://doi.org/10.1016/S0166-445X(02)00007-3
  6. Kim, T.H., Yang, M.H., Choe, M.K., Han, S.J. and Yeu, I.K. (2005) Physiological studies on acute water-temperature stress of juvenile abalone, Haliotis discus hannai. The Korean Journal of Aquaculture, 18(1): 7-12.
  7. Kim, T.H., Kim, K.J., Choe, M.K. and Yeu, I.K. (2006) Physiological changes of juvnvile abalone, Haliotis sieboldii exposed to acute water-temperature stress. The Korean Journal of Aquaculture, 19(2): 77-83.
  8. KNSO (2011) Korea National Statistical Office. Fishery Production Survey DB, Daejeon, Korea.
  9. Moody, C.S. and Hassan, H.M. (1982) Mutagenicity of oxygen free radicals. Proceedings of the National Academy of Sciences, 79: 2855-2859. https://doi.org/10.1073/pnas.79.9.2855
  10. Parihar, M.S., Dubey, A.K., Javeri, T. and Prakash, P. (1996) Changes in lipid peroxidation, superoxide dismutase activity, ascorbic acid and phospholipid content in liver of freshwater catfish heteropneustes fossilis exposed to elevated temperature. Jounal of Thermal Biology, 21: 323-330. https://doi.org/10.1016/S0306-4565(96)00016-2
  11. Parihar, M.S., Javeri, T., Hemnani, T., Dubey, A.K. and Prekash, P. (1997) Respinses of superoxide dis mutate, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the freshwater catfish (heteropneustes fossilis) to short-term elevated temperature. Journal of Thermal Biology, 22: 151-156. https://doi.org/10.1016/S0306-4565(97)00006-5
  12. Riche, M. (2007) Analysis of refractometry for determining total plasma protein in hybrid striped bass (Morone chrysops${\times}$M. saxatilis) at various salinities. Aquaculture, 264: 279-284. https://doi.org/10.1016/j.aquaculture.2006.12.018
  13. Simon, R.H., Scoggin, C.H. and Patterson, D. (1981) Hydrogen peroxide causes the fatal injury to human fibroblast exposed to oxygen radicals. Journal of Biologicla Chemistry, 256: 7181-7186.
  14. Wedemeyer, G.A. and McLeay, D.J. (1981) Methods for determining the tolerance of fishes to environmental stressors. In Stress and Fish A.D. Pickering, ed. Academic Press, London, pp. 247-275.
  15. Wendel, A. and Feuerstein, S. (1981) Drug-induced lipid peroxidation in mice-1. Modulation by monoxygenase activity, glutathione and selenium status. Biochemical Pharmacology, 30: 2513-2550. https://doi.org/10.1016/0006-2952(81)90576-1
  16. Yang, H.S., Park, K.I., Hong, C.H. and Choi, K.S. (2008) Effects of salinity stress on the composition of the free amino acids of the Pacific abalone Haliotis discus discus. The Korean Journal of Aquaculture, 2(4): 218-225.

피인용 문헌

  1. Changes of enzyme activity in the hemolymph and hepatopancreas of the abalone, Haliotis discus hannai (Ino, 1953) exposed to cadmium vol.30, pp.1, 2014, https://doi.org/10.9710/kjm.2014.30.1.41
  2. Analysis of Current Distribution around a Scaled-down Abalone System to determine the cause of mass mortality of abalone, Haliotis discus hannai (Ino, 1952) vol.30, pp.1, 2014, https://doi.org/10.9710/kjm.2014.30.1.9
  3. Myopathy in Clinically Healthy Cultured Abalone Haliotis discus hannai vol.47, pp.2, 2014, https://doi.org/10.5657/KFAS.2014.0174
  4. Influence of Elevated Temperatures on the Physiological Response of Hemolymph from Two Species of the Abalone, Haliotis discus hannai and H. discus discus vol.31, pp.1, 2015, https://doi.org/10.9710/kjm.2015.31.1.1
  5. Influence of elevated temperatures on the physiological response of hemolymph from two species of abalone, Haliotis gigantea and Haliotis discus discus (Reeve, 1846) vol.31, pp.3, 2015, https://doi.org/10.9710/kjm.2015.31.3.187
  6. ) vol.31, pp.4, 2015, https://doi.org/10.9710/kjm.2015.31.4.285
  7. Survival Rate and Hematological Responses with Temperature Changes of Red Spotted Grouper, Epinephelus akaara in South Korea vol.20, pp.2, 2016, https://doi.org/10.12717/dr.2016.20.2.081
  8. Survival Rate and Hematological Responses with Temperature Changes of Red Spotted Grouper, Epinephelus akaara in South Korea vol.20, pp.2, 2011, https://doi.org/10.12717/dr.2016.20.2.103
  9. 북방전복 (Haliotis discus hannai) 과 둥근전복 (H. discus discus) 의 종내 교잡에 따른 순종과 교잡종의 성장과 생존 비교 vol.34, pp.1, 2018, https://doi.org/10.9710/kjm.2018.34.1.31