Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지

Specific Marker Gene Analyses for DNA Polymorphism of the Blood Cell in Korea Native Brindled Cattle

칡한우 혈액에서 DNA 다양성 분석을 통한 표지 유전자 탐색

  • Kim, Sang-Hwan (Dept. of Animal Life Science, Hankyong National University) ;
  • Hong, Yeon-Sik (Dept. of Animal Life Science, Hankyong National University) ;
  • Lee, Ho-Joun (Institute of Genetic Engineering, Hankyong National University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 김상환 (국립한경대학교 생물환경정보통신 전문대학원 동물생명공학) ;
  • 홍연식 (국립한경대학교 생물환경정보통신 전문대학원 동물생명공학) ;
  • 이호준 (국립한경대학교 유전공학연구소) ;
  • 윤종택 (국립한경대학교 유전공학연구소)
  • Received : 2011.11.10
  • Accepted : 2011.12.01
  • Published : 2011.12.31
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Abstract

This study was conducted to detect the specific expressing genes by using RAPD-PCR and RFLP method in the Korea Native Brindled Cattle, Korean Native cow and Holstein cattle. And then, the specific marker gene was investigated by the analysis of the genes for detection significance according to the expressing pattern. We found the specific expression gene by the RAPD-PCR analysis in Korea Native Brindled Cattle. It was detected the differences of the species in the colour and external section. The Korea Native Brindled Cattle were vary low compare to the Korean Native cow and Holstein cattle by analysis result of polymorphism and distribution. And there were a found the specific marker gene by sequencing in the R9B gene fragment of Korea Native Brindled Cattle. And the sequencing result of the R9B was different between Korean Native cow and Holstein cattle. Thus, this gene can be apply as the specific marker gene in the Korea Native Brindled Cattle.

본 연구는 칡소와 한우 그리고 젖소의 각 군을 통하여 RAPD-PCR방법과 RFLP방법을 응용하여 칡소에서 특이적으로 발현되는 유전자의 검출과 발현빈도에 따른 표지유전자를 분석하여 칡소 특이적인 표지인자를 탐색하고자 실시하였다. 연구결과, RAPD분석을 통하여 칡소에서 특이적으로 표현되는 유전자들을 발견할 수 있었으며, 검출 유전자의 다양성이 모색과 종간의 차이가 있음을 알 수 있었다. 특이적으로 표현된 유전자들 중 칡소에서 특이적으로 표현되는 R9B 유전자를 발견할 수 있었고, 이 유전자는 한우와 젖소의 일부 DNA 염기서열상의 차이점이 있음을 확인할 수 있었으며, 추후 칡소의 표지유전자로 적용할 수 있을 것이라 사료되었다.

Keywords

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