Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지

Effects of Feeding Nattokinase as Natural Feed Additives on Milk Production and Blood Metabolites in Lactating Dairy Cows

천연 사료첨가제 Nattokinase 공급에 따른 젖소의 산유능력 및 혈액성상에 미치는 영향

  • Received : 2011.10.18
  • Accepted : 2011.12.21
  • Published : 2011.12.31
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Abstract

This experiment was conducted to determine the effect of nattokinase (NK) additives on milk production and composition, and blood metabolites in dairy cows. The two kinds of nattokinase with high fibrinolytic activity were produced by two strains of bacteria, Bacillus amyloliquefacines (NK1) and Bacillus subtilis (NK2). Total fifteen Holstein cows (average $1.83{\pm}0.37$ parity; average milk yield $23.2{\pm}3.2$ kg/d) were randomly assigned to three treatments (5 animals per treatment). Cows were fed TMR supplemented with 0g, 100g and 100g for control, NK1 and NK2 treatment, respectively for 4 weeks. Milk yield was significantly higher (p<0.05) for NK1 (22.89 kg/d) than for control (21.07 kg/d) and NK2 (21.36 kg/d). Somatic cell counts in NK treatments were significantly lower than that in control group (58,000 vs. 21,000 and 35,000 cells/ml, control vs. NK1 and NK2). Serum ALT levels in all treatment were similar to the range of 32.00~35.83 IU/L, but AST levels in NK1 (85.67 IU/L) was significantly decreased compared with those in control and NK2 (121.67 and 117.67 IU/L respectively). Serum T-CHO levels in NK1 (145.33 mg/dl) was significantly decreased (p<0.05) compared with that in control (179.00 mg/dl) and NK2 (176.17 mg/dl). This finding showed that NK1 additives could possibly have a positive effect in lactation performance of mid-lactation dairy cows by increasing milk yield, reducing somatic cell count, improving liver function and decreasing cholesterol in blood.

Keywords

References

  1. 박수봉․김현섭․김창근․정영채․이종완․김천호. 1997. 젖소의 수태율과 혈장 요소태질소의 관계. 한국가축번식학회지 21: 185-189.
  2. 안종호․조익환․이주삼. 2003. 유럽의 유기축산 사례 및 우리나라 유기축산의 발전 방안. 한국유기농업학회지 11: 75-92.
  3. 오상집. 2003. 친환경 축산물 생산을 위한 사료의 이용 효과. 제4회 국제심포지엄, 친환경축산물 생산을 위한 사료의 이용, 개발. 사단법인 한국단미사료협회.
  4. 이종완․정영채․김창근․김명희․최선호. 2007. 젖소에 있어서 분만 후 혈액 및 우유의 성분 변화와 성분 간의 상관관계. 한국수정란이식학회지 22: 127-135.
  5. 須見洋行. 1991. ナットウキナーゼと線溶系. 化学と生物 29: 119-123.
  6. Ahn, Y. S., Y. S. Kim, and D. H. Shin. 2006. Isolation, identification and fermentation characteristics os Bacillus sp. with high protease activity from traditional cheonggukjang. Kor. J. Food Sci. Technol. 38: 82-87.
  7. AOAC. 1995. Official Methods of Analysis. 16th Ed. Association of Official Analytical Chemist, Washington DC., USA.
  8. Bernard, F. G., Z. Alexandre, M. Robert, and M. Catherine. 2004. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Res. International 37: 123-131. https://doi.org/10.1016/j.foodres.2003.09.010
  9. Blowey, R. W., D. W. Wood, and Davis, Jr. 1973. A nutritional monitoring system for dairy herds based on blood glucose, urea and albumin levels. Vet. Rec. 92: 691-696. https://doi.org/10.1136/vr.92.26.691
  10. Carroll, D. J., B. A. Barton, G. W. Anderson, and R. D. Smith. 1988. Influence of protein intake and feeding strategy on reproductive performance of dairy cows. J. Dairy Sci. 71: 3470-3481. https://doi.org/10.3168/jds.S0022-0302(88)79953-1
  11. Diaz, G. J., E. J. Squires, and R. J. Julian. 1999. The use of selected plasma enzyme activities for the diagnosis of fatty liver-hemorrhagic syndrome in laying hens. Avian Disease. 43: 768-773. https://doi.org/10.2307/1592746
  12. Ferguson, J. D., D. T. Galligan, T. Blanchard, and N. Reeves. 1993. Serum urea nitrogen and conception rate: The usefulness of test information. J. Dairy Sci. 76: 3742-3746. https://doi.org/10.3168/jds.S0022-0302(93)77716-4
  13. Grummer, R. R. and D. J. Carroll. 1991. Effects of dietary fat on metabolic disorders and reproductive performance of dairy cattle. J. Anim. Sci. 69: 3838-3852. https://doi.org/10.2527/1991.6993838x
  14. Harms, R. H. and C. F. Simpson. 1979. Serum and body characteristics of laying hens with fatty liver syndrome. Poult. Sci. 58: 1644-1646. https://doi.org/10.3382/ps.0581644
  15. Hawkins, D. E., K. D. Niswender, G. M. Oss, C. L. Moeller, K. G. Odde, H. R. Sawyer and G. D. Niswender. 1995. An increase serum lipids increases luteal lipid content and alters the disappearance rate of progesterone in cows. J. Anim. Sci. 73: 541-545. https://doi.org/10.2527/1995.732541x
  16. Iwai, K., N. Nakaya, Y. Kawasaki, and H. Matsue. 2002. Antioxidative functions of natto, a kind of fermented soybeans: effect on LDL oxidation and lipid metabolism in cholesterolfed rats. J. Agric. Food Chem. 50: 3597-3601. https://doi.org/10.1021/jf0117199
  17. Japan Functional Food Research Association. 1999. "Fibrinolytic Activator of Nattokinase (in Japanese)", ART Co. Ltd., Tokyo.
  18. Jordan, E. R., T. E. Chapman, P. E. Hoitan, and L. V. Swanson. 1983. Relationship of dietary crude protein to composition of uterine secretion and blood in high-producing postpartum dairy cows. J. Dairy Sci. 66: 1854-1862. https://doi.org/10.3168/jds.S0022-0302(83)82023-2
  19. Kappel, L. C., R. H. Ingraham, E. B. Morgan, L. Zeringue, D. Wilson, and D. K. Babcock, 1984. Relationship between fertility and blood glucose and cholesterol concentrations in Holstein cows. Anim. J. Vet. Res. 45: 2607-2612.
  20. Kweon, O. K., H. Ono, K. Osasa, M. Onda, K. Oboshi, H. Uchisugi, S. Kurosawa, H. Yamashina, and H. Kanagawa. 1986. Factors affecting serum total cholesterol level of lactating Holstein cows. Nippon Juigaku Zasshi. 48: 481-486. https://doi.org/10.1292/jvms1939.48.481
  21. Lammoglia, M. A., S. T. Willard, J. R. Oldham, and R. D. Randel. 1996. Effects of dietary fat and season on steroid hormonal profiles before parturition and on hormonal, cholesterol, triglycerides, follicular patterns, and postpartum reproduction in Brahman cows. J. Anim. Sci. 74: 2253-2262. https://doi.org/10.2527/1996.7492253x
  22. Lee, Y. N., M. J. Sin, and B. N. Kim. 1991. A study on the present state of traditional food. Kor. J. Dietary Culture. 6: 71-81.
  23. Lumeij J. T. 1997. Avian clinical biochemistry. Pages 857-883 In: Clinical Biochemistry of Domestic Animals, 5th Ed. Kaneko JJ, Harvey JW, Bruss ML Ed. Academic Press, San Diego, CA.
  24. Oh, J. H., B. J. Lee, H. R. Paik, S. C. Jung, K. S. Baik, and S. K. Choi. 2009. Isolation of bacteria from chuggukjang prepared by rice straw and identification of protease secreted. J. Life Sci. 19: 397-402. https://doi.org/10.5352/JLS.2009.19.3.397
  25. Peng, H., J. Q. Wang, H. Y. Kang, S. H. Dong, P. Sun, D. P. Bu, and L. Y. Zhou. 2011. Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows. J. Anim. Physiol. Anim. Nutr(Berl). Jun 3. 21635575.
  26. Roseler, D. K., J. D. Ferguson, C. J. Sniffen, and J. Herrema. 1993. Dietary protein degradability effects on plasma and milk urea nitrogen and milk nonprotein nitrogen in Holstein cows. J. Dairy Sci. 76: 525-530. https://doi.org/10.3168/jds.S0022-0302(93)77372-5
  27. SAS. 2000. $SAS/STAT^{(R)}$ Software for PC. SAS Institute Inc., Cary, NC, USA.
  28. Sonderman, J. P. and L. L. Larson. 1989. Effect of dietary protein and exogenous gonadotropin releasing hormone on circulating progesterone concentrations and performance of Holstein cows. J. Dairy Sci. 72: 2179-2184. https://doi.org/10.3168/jds.S0022-0302(89)79343-7
  29. Sumi, H. 1987. Development of nattokinase and healthy natto (in Japanese). Bioindustry. 7: 725-731.
  30. Sumi, H., H. Hamada, H. Tsushima, H. Mihara, and H. Muraki. 1987. A novel fibrinolytic enzyme (Nattokinase) in the vegetable cheese Natto: A typical and popular soybean food in the japanese diet. Experimentia. 43: 1110-1111. https://doi.org/10.1007/BF01956052
  31. Sun, P., J. Q. Wang, and H. T. Zhang. 2010. Effects of Bacillus subtilis natto on performance and immune function of preweaning calves. J. Dairy Sci. 93: 5851-5855. https://doi.org/10.3168/jds.2010-3263
  32. Van Soest P. J. 1982. Nutritional ecology of the ruminant. Cornell University Press, Ithaca, NY., USA.
  33. Yokota, Y., T. Hattori, H. Ohishi, K. Hasegawa, and K. Watanabe. 1996. The effect of antioxidant-containing fraction from fermented soybean food on atherosclerosis development in cholesterol-fed rabbits. Lebensm. Wiss. u. Technol. 29: 751-755. https://doi.org/10.1006/fstl.1996.0117