Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis of Compounds in Fermented Insampaedok-san and Their Neuroprotective Activity in HT22 Cells

  • Weon, Jin-Bae (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Jin-Yeul (TKM Converging Research Division, Korea Institute of Oriental Medicine) ;
  • Yang, Hye-Jin (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Choong-Je (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University)
  • Received : 2010.12.31
  • Accepted : 2011.03.18
  • Published : 2011.03.31
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Abstract

Insampaedok-san is a traditional medicine used for the treatment of colds. We investigated several compounds in Insampeadok-san, and tested their neuroprotective and anti-oxidative activities after fermentation with Lactobacillus. The amounts of four marker compounds (ferulic acid, hesperidin, 6-gingerol and glycyrrhizin) and unidentified compounds in Insampaedok-san (IS) and fermented Insampaedok-san (FIS) were measured and compared by an established HPLC-DAD method. Neuroprotective activity of IS and FIS extracts was evaluated and compared after glutamate-induced neurotoxicity in HT22 cells. Anti-oxidative activity of IS and FIS was also compared in DPPH free radical, hydroxyl radical and hydrogen peroxide scavenging activity assays. Contents of two compounds, ferulic acid and glycyrrhizin were decreased while 6-gingerol was increased by fermentation. FIS showed more potent neuroprotective activity than IS. DPPH, hydroxyl radical and hydrogen peroxide scavenging was slightly increased by FIS when compared to IS. In conclusion, fermentation with Lactobacillus can vary the amounts of the marker compounds in IS and improve neuroprotective and anti-oxidative activities of IS.

Keywords

References

  1. Braun, S., Liebetrau, W., Berning, B., and Behl, C., Dexamethasoneenhanced sensitivity of mouse hippocampal HT22 cells for oxidative stress is associated with the suppression of nuclear factor-kappaB. Neurosci. Lett. 295(3), 101-104 (2000). https://doi.org/10.1016/S0304-3940(00)01603-7
  2. Chen, X., Fruehauf, J., Goldsmith, J.D., Xu, H., Katchar, K.K., Koon, H.W., Zhao, D., Kokkotou, E.G., Pothoulakis, C., and Kelly, C.P., Saccharomyces boulardii inhibits EGF receptor signaling and intestinal tumor growth in Apc(min) mice. Gastroenterol. 137(3), 914-923 (2009). https://doi.org/10.1053/j.gastro.2009.05.050
  3. Choi, D.W., Glutamate neurotoxicity and diseases of the nervous system. Neuron. 1, 623-634 (1998).
  4. Coyle, J.T. and Puttfarcken, P., Oxidative stress, glutamate, and neurodegenerative disorders. Science. 262, 689-695 (1993). https://doi.org/10.1126/science.7901908
  5. Davis, J.B. and Maher, P., Protein kinase C activation inhibits glutamateinduced cytotoxicity in a neuronal cell line. Brain Res. 652(1), 169-173 (1994). https://doi.org/10.1016/0006-8993(94)90334-4
  6. Doh, E.S., Chang, J.P., Lee, K.H., and Seong, N.S., Ginsenoside change and antioxidation activity of fermented ginseng. Kor. J. Medicinal Crop Sci. 18(4), 255-266 (2010).
  7. Fukui, M., Song, J.-H., Choi, J., Choi, H.J., and Zhu, B.T., Mechanism of glutamate-induced neurotoxicity in HT22 mouse hippocampal cells. Eur. J. Pharmacol. 617(1-3), 1-11 (2009). https://doi.org/10.1016/j.ejphar.2009.06.059
  8. Goldin, B.R., Health benefits of probiotics. Br. J. Nutr. 80, 203-207 (1998).
  9. Hyon, J.-S., Kang, S.-My., Han, S.-W., Kang, M.-C., Oh, M.-C., Oh, C.- K., and Kim, D.-W., Jeon, Y.-J., and Kim, S.-H., Flavonoid Component changes and antioxidant activities of fermented Citrus grandis Osbeck Peel. J. Kor. Soc. Food Sci. Nutr. 38(10), 1310-1316 (2009). https://doi.org/10.3746/jkfn.2009.38.10.1310
  10. Jiang, W.-Y., Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science. Trends Pharmacol. Sci. 26, 558-563 (2005). https://doi.org/10.1016/j.tips.2005.09.006
  11. Kim, J.-H., Lee, W.-J., Cho, Y.-W., and Kim. K.-Y., Storage-life and palatability extension of Betula platyphylla Sap using lactic acid bacteria fermentation. J. Kor. Soc. Food Sci. Nutr. 38(6), 787-794 (2009). https://doi.org/10.3746/jkfn.2009.38.6.787
  12. Liu, J., Li, L., and Suo, W.Z., HT22 hippocampal neuronal cell line possesses functional cholinergic properties. Life Sci. 84(9-10), 267-271 (2009). https://doi.org/10.1016/j.lfs.2008.12.008
  13. Satoh, T., Enokido, Y., Kubo, T., Yamada, M., and Hatanaka, H., Oxygen toxicity induces apoptosis in neuronal cells. Cell. Mol. Neurobiol.18(6), 649-666 (1998). https://doi.org/10.1023/A:1020269802315
  14. Smith, C.D., Carney, J.M., Starke-Reed, P.E., and Oliver, C.N., Stadtman, E.R., Floyd, R.A., Markesbery, W.R., Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease. Proc. Natl. Acad. Sci. USA. 88(23), 10540-10543 (1991). https://doi.org/10.1073/pnas.88.23.10540
  15. Shim, J.Y. and Kim B.W., A study of effects of Insampaedok-san and Kammiinsampaedok san on the analgesia, antipyresis and thioacetamide induced liver damage in rats and mice. K. H. Univ. O. Med. J., 7, 323-334 (1984).