Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Comparison of the Scrophulariae Radix of Gyeongbuk's with that of Chinese: Content of Harpagoside and Inhibitory Effect of Nitric Oxide

경북산 현삼과 중국산 현삼의 비교: Harpagoside 함량 및 Nitric Oxide 저해활성

  • Received : 2010.11.25
  • Accepted : 2011.01.20
  • Published : 2011.03.31
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Abstract

Scrophulariae Radix (Scrophulariaceae) has been traditionally used to treat pharyngitis, laryngitis, tonsillitis, fever, and edema. To compare the quality of the Scrophulariae Radix produced from Gyeongbuk with that from China, a quantitative analysis on the major component, E-harpagoside, was performed by reversed-phase HPLC, and a relative inhibitory activity of nitric oxide (NO) was assayed in the Raw 264.7 cells. Using a Luna $C_{18}$(2) column, we carried out quantitative analysis with a gradient of 0.1% phosphoric acid in water and acetonitrile. Our results demonstrated that the content of E-harpagoside in Gyeongbuk's Scrophulariae Radix were three times higher than that of Chinese. Moreover, the inhibitory activity of Scrophulariae Radix of Gyeongbuk's in the lipopolysaccharide (LPS)-induced NO production was higher than that of Chinese.

Keywords

References

  1. 식품의약품안전청 (2008) 대한약전외한약(생약)규격집 제8개정, 460. 서울.
  2. 국가약전위원회 (2005) 중화인민공화국약전, 76-77. 화학공업출판사, 북경.
  3. 우미희 (1989) 玄蔘의 成分에 關한 硏究. 曉星基科集 3: 129-144.
  4. 전국한의과대학본초학교수 공편저 (2000) 본초학, 192-193. 영림사, 서울.
  5. 생약학교재편찬위원회 (2006) 생약학, 208. 동명사, 서울.
  6. 이영종, 안덕균 (1986) 현삼의 기원과 효능에 관한 고찰. 대한한의학회 본초분과학회지 1: 63-72.
  7. 박규하, 함인혜, 최호영 (2008) 현삼의 유전자 분석 및 자발성고혈압흰쥐의 혈압에 미치는 영향. 대한본초학회지 23: 213-223.
  8. 황원상, 김민주, 오수진, 박성훈, 강홍준, 김재현, 이관용, 홍수희, 신현경, 원무호, 서준교, 오양석, 김종일, 김재봉, 박재봉, 이재용 (2006) 현삼(Scrophularia koraiensis Nakai) 의 항노화 활성. 한국노화학회지 16: 37-46.
  9. 한방 약리학 교재편찬위원회 (2005) 한방 약리학, 693-695, 신일상사, 서울.
  10. Li. Y. M., Jiang. S. H., Gao. W. Y. and Zhu. D. Y. (1999) Iridoid glycosides from Scrophularia ningpoensis. Phytochemistry 50: 101-104. https://doi.org/10.1016/S0031-9422(98)00477-4
  11. 이혜원, 박소영, 추병길, 천진미, 이아영, 김호경 (2007) 국산과 중국산 현삼의 성분함량. 생약학회지 38: 15-18.
  12. Kim, S. R., Lee, K. Y., Koo, K. A., Sung, S. H., Lee, N. G., Kim, J. and Kim, Y .C. (2002) Four new neuroprotective iridoid glycosides from Scrophularia buergeriana Roots. J. Nat. Prod. 65: 1696-1699. https://doi.org/10.1021/np0202172
  13. Kim, S. R. and Kim, Y. C. (2000) Neuroprotective phenylpropanoid esters of rhamnose isolated from roots of Scrophularia buergeriana. Phytochemistry 54: 503-509. https://doi.org/10.1016/S0031-9422(00)00110-2
  14. Kim, S. R., Kang, S. Y., Lee, K. Y., Kim, S. H., Markelonis, G. J., Oh, T. H. and Kim, Y. C. (2003) Anti-amnestic activity of E-P-methoxycinnamic acid from Scrophularia buergeriana. Cognitive Brain Research 17: 454-461. https://doi.org/10.1016/S0926-6410(03)00161-7
  15. Kim, S. R., Sung, S. H., Jang, Y. P., Markelonis, G. J., Oh, T. H. and Kim, Y. C. (2002) E-p-Methoxycinnamic acid protects cultured neuronal cells against neurotoxicity induced by glutamate. Br. J. Pharmacol. 135: 1281-1291. https://doi.org/10.1038/sj.bjp.0704576
  16. 박용기, 강병수 (1998) 현삼의 항산화 작용에 관한 연구. 대한본초학회지 13: 201-220.
  17. Xu, C., Luo, L. and Tan, R. X. (2004) Antidepressant effect of three traditional Chinese medicines in the learned helplessness model. J. Ethnopharmacol. 91: 345-349. https://doi.org/10.1016/j.jep.2004.01.012
  18. Diaz, A. M., Abad, M. J., Fernandez, L., Silvan, A. M., De Santos, J. and Bermejo, P. (2004) Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sci. 74: 2515-2526. https://doi.org/10.1016/j.lfs.2003.10.008
  19. Ahmed, B., Al-Rehaily, A. J., A1-Howiriny, T. A., El-Sayed, K. A. and Ahmad, M. S. (2003) Scropolioside-D2 and harpagoside-B: two new iridoid glycosides and antiinflammatory activity. Biol. Pharm. Bull. 26: 462-467. https://doi.org/10.1248/bpb.26.462
  20. Lee, E. J., Kim, S. R., Kim, J. and Kim Y. C. (2002) Hepatoprotective Phenylpropanoids from Scrophularia buergeriana roots against $CCI_4-Induced$ toxicity: Action mechanism and structure-activity relationship. Planta Med. 68: 407-411. https://doi.org/10.1055/s-2002-32081
  21. Tohda, C., Kakihara, Y., Komatsu, K. and Kuraishi, Y. (2000) lnhibitory effects of methanol extracts of herbal medicines on substance P-induced itch-scratch response. Biol. Pharm. Bull. 23: 599-601. https://doi.org/10.1248/bpb.23.599
  22. 변성희, 양재하, 김상찬 (2005) 현삼메탄올 추출물이 LPS로 유도된 Raw 264.7 cell에서의 $TNF-{\alpha}$, IL $2{\beta}$, IL 6 및 nitic oxide 생성에 미치는 영향. 대한본초학회지 20: 7-16.
  23. 농산물유통국 채소특작과 (2006) 경북지역 한약재 현황.
  24. 농산물유통국 채소특작과 (2006) 국내 한약재 생산현황.
  25. 대구경북한방산업진흥원 (2007) 대구경북한방산업진흥원 보고서.
  26. 한국의약품수출입협회 (2005) 한약재수출입현황.
  27. Huang, T. H., Tran, V. H., Duke, R. K., Tan, S., Chrubasik, S., Roufogalis, B. D. and Duke, C. C. (2006) Harpagoside suppresses lipopolysaccharide-induced iNOS and COX-2 expression through inhibition of $NF-{\kappa}B$ activation. J. Ethnophamacol. 104: 149-155. https://doi.org/10.1016/j.jep.2005.08.055