IMMUNE NETWORK

The Korean Association of Immunobiologists (대한면역학회)
권호 표지
DOI QR코드

DOI QR Code

Th17 Responses Are not Induced in Dextran Sodium Sulfate Model of Acute Colitis

  • Kim, Yoon-Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju) ;
  • Lee, Min-Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju) ;
  • Ju, Ahn-Seung (Department of Biomedical Laboratory Science, Daegu Health College) ;
  • Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju)
  • Received : 2011.10.07
  • Accepted : 2011.11.03
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-${\gamma}$ (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.

Keywords

References

  1. Xavier RJ, Podolsky DK: Unravelling the pathogenesis of inflammatory bowel disease. Nature 448;427-434, 2007. https://doi.org/10.1038/nature06005
  2. Strober W, Fuss I, Mannon P: The fundamental basis of inflammatory bowel disease. J Clin Invest 117;514-521, 2007. https://doi.org/10.1172/JCI30587
  3. Strober W: Why study animal models of IBD? Inflamm Bowel Dis 14(Suppl 2);S129-S131, 2008. https://doi.org/10.1002/ibd.20667
  4. Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R: A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98;694-702, 1990. https://doi.org/10.1016/0016-5085(90)90290-H
  5. Pizarro TT, Cominelli F: Cytokine therapy for Crohn's disease: advances in translational research. Annu Rev Med 58;433-444, 2007. https://doi.org/10.1146/annurev.med.58.121205.100607
  6. Fuss IJ, Becker C, Yang Z, Groden C, Hornung RL, Heller F, Neurath MF, Strober W, Mannon PJ: Both IL-12p70 and IL-23 are synthesized during active Crohn's disease and are down-regulated by treatment with anti-IL-12 p40 monoclonal antibody. Inflamm Bowel Dis 12;9-15, 2006. https://doi.org/10.1097/01.MIB.0000194183.92671.b6
  7. Alex P, Zachos NC, Nguyen T, Gonzales L, Chen TE, Conklin LS, Centola M, Li X: Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis. Inflamm Bowel Dis 15;341-352, 2009. https://doi.org/10.1002/ibd.20753
  8. Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL: A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses. Nat Med 15;1016-1022, 2009. https://doi.org/10.1038/nm.2015
  9. Brown JB, Cheresh P, Zhang Z, Ryu H, Managlia E, Barrett TA: P-selectin glycoprotein ligand-1 is needed for sequential recruitment of T-helper 1 (Th1) and local generation of Th17 T cells in dextran sodium sulfate (DSS) colitis. Inflamm Bowel Dis 2011. [Epub ahead of print]
  10. Sanada Y, Mizushima T, Kai Y, Nishimura J, Hagiya H, Kurata H, Mizuno H, Uejima E, Ito T: Therapeutic effects of novel sphingosine-1-phosphate receptor agonist W-061 in murine DSS colitis. PLoS One 6;e23933, 2011. https://doi.org/10.1371/journal.pone.0023933
  11. Nunez-Andrade N, Lamana A, Sancho D, Gisbert JP, Gonzalez-Amaro R, Sanchez-Madrid F, Urzainqui A: P-selectin glycoprotein ligand-1 modulates immune inflammatory responses in the enteric lamina propria. J Pathol 224;212-221, 2011. https://doi.org/10.1002/path.2850
  12. Ito R, Kita M, Shin-Ya M, Kishida T, Urano A, Takada R, Sakagami J, Imanishi J, Iwakura Y, Okanoue T, Yoshikawa T, Kataoka K, Mazda O: Involvement of IL-17A in the pathogenesis of DSS-induced colitis in mice. Biochem Biophys Res Commun 377;12-16, 2008. https://doi.org/10.1016/j.bbrc.2008.09.019
  13. Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE, Büchler MW: Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion 62;240-248, 2000. https://doi.org/10.1159/000007822
  14. Nam JS, Terabe M, Kang MJ, Chae H, Voong N, Yang YA, Laurence A, Michalowska A, Mamura M, Lonning S, Berzofsky JA, Wakefield LM: Transforming growth factor beta subverts the immune system into directly promoting tumor growth through interleukin-17. Cancer Res 68;3915-3923, 2008. https://doi.org/10.1158/0008-5472.CAN-08-0206
  15. Pichavant M, Goya S, Meyer EH, Johnston RA, Kim HY, Matangkasombut P, Zhu M, Iwakura Y, Savage PB, DeKruyff RH, Shore SA, Umetsu DT: Ozone exposure in a mouse model induces airway hyperreactivity that requires the presence of natural killer T cells and IL-17. J Exp Med 205;385-393, 2008. https://doi.org/10.1084/jem.20071507
  16. Rachitskaya AV, Hansen AM, Horai R, Li Z, Villasmil R, Luger D, Nussenblatt RB, Caspi RR: Cutting edge: NKT cells constitutively express IL-23 receptor and RORgammat and rapidly produce IL-17 upon receptor ligation in an IL-6-independent fashion. J Immunol 180;5167-5171, 2008. https://doi.org/10.4049/jimmunol.180.8.5167
  17. Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A: IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger. J Immunol 170;2106-2112, 2003. https://doi.org/10.4049/jimmunol.170.4.2106
  18. Takahashi N, Vanlaere I, de Rycke R, Cauwels A, Joosten LA, Lubberts E, van den Berg WB, Libert C: IL-17 produced by Paneth cells drives TNF-induced shock. J Exp Med 205;1755- 1761, 2008. https://doi.org/10.1084/jem.20080588
  19. Duan J, Chung H, Troy E, Kasper DL: Microbial colonization drives expansion of IL-1 receptor 1-expressing and IL-17-producing gamma/delta T cells. Cell Host Microbe 7;140-150, 2010. https://doi.org/10.1016/j.chom.2010.01.005

Cited by

  1. Immunoproteasome Subunit LMP7 Deficiency and Inhibition Suppresses Th1 and Th17 but Enhances Regulatory T Cell Differentiation vol.189, pp.8, 2012, https://doi.org/10.4049/jimmunol.1201183
  2. Protective Effect of Laminaria japonica with Probiotics on Murine Colitis vol.2014, pp.None, 2014, https://doi.org/10.1155/2014/417814
  3. Intravenousvsintraperitoneal mesenchymal stem cells administration: What is the best route for treating experimental colitis? vol.20, pp.48, 2011, https://doi.org/10.3748/wjg.v20.i48.18228
  4. Ginseng Berry Extract Attenuates Dextran Sodium Sulfate-Induced Acute and Chronic Colitis vol.8, pp.4, 2011, https://doi.org/10.3390/nu8040199
  5. Oral tolerance is inducible during active dextran sulfate sodium-induced colitis vol.7, pp.2, 2016, https://doi.org/10.4292/wjgpt.v7.i2.242
  6. Over-Expression of CD200 Protects Mice from Dextran Sodium Sulfate Induced Colitis vol.11, pp.2, 2016, https://doi.org/10.1371/journal.pone.0146681
  7. The Bisphosphonate Pamidronate is an Intestinal Antiinflammatory Agent in Rat and Mouse Experimental Colitis vol.22, pp.11, 2011, https://doi.org/10.1097/mib.0000000000000920
  8. Amelioration of Experimental autoimmune encephalomyelitis and DSS induced colitis by NTG-A-009 through the inhibition of Th1 and Th17 cells differentiation vol.8, pp.None, 2011, https://doi.org/10.1038/s41598-018-26088-y