Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Characterization and Morphology of Two Endophytic Peyronellaea Species from Pinus koraiensis in Korea

  • Deng, Jian Xin (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Paul, Narayan Chandra (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Li, Mei Jia (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Department Administration) ;
  • Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2011.10.06
  • Accepted : 2011.10.23
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Species of Phoma and its allies were isolated during a survey on the diversity of endophytic fungi associated with pine trees in Korea. Based on the phylogenetic analyses of internal transcribed spacer and ${\beta}$-tubulin gene sequences, two Phoma-like species from the needles of Pinus koraiensis were identified as Peyronellaea calorpreferens and P. glomerata. They were also morphologically identified based on the previous descriptions. Here, we report P. calorpreferens and P. glomerata being present in Korea as endophytic fungi in Pinus koraiensis.

Keywords

References

  1. Aveskamp MM, de Gruyter J, Woudenberg JH, Verkley GJ, Crous PW. Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera. Stud Mycol 2010;65:1-60. https://doi.org/10.3114/sim.2010.65.01
  2. Boerema GH, de Gruyter J, Noordeloos ME, Hamers ME. Phoma identification manual: differentiation of specific and infra-specific taxa in culture. Wallingford: CABI Publishing; 2004.
  3. Punithalingam E. Book review: conidial fungi, Phoma identification manual: differentiation of specific and infra-specific taxa in culture. Mycotaxon 2004;90:487-93.
  4. Torres MS, Bergen M, Singh S, Bischoff J, Sullivan RF, White JF Jr. Plenodomus morganjonesii sp. nov. and a discussion of the genus Plenodomus. Mycotaxon 2005;93:333-43.
  5. Aveskamp MM, Verkley GJ, de Gruyter J, Murace MA, Perello A, Woudenberg JH, Groenewald JZ, Crous PW. DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties. Mycologia 2009;101:363-82. https://doi.org/10.3852/08-199
  6. Aveskamp MM, de Gruyter J, Crous PW. Biology and recent developments in the systematics of Phoma, a complex genus of major quarantine significance. Fungal Divers 2008;31:1-18.
  7. de Gruyter, J, Aveskamp MM, Woudenberg JH, Verkley GJ, Groenewald JZ, Crous PW. Molecular phylogeny of Phoma and allied anamorph genera: towards a reclassification of the Phoma complex. Mycol Res 2009;113(Pt 4):508-19. https://doi.org/10.1016/j.mycres.2009.01.002
  8. Irinyi L, Kovics GJ, Sandor E. Taxonomical re-evaluation of Phoma-like soybean pathogenic fungi. Mycol Res 2009;113(Pt 2):249-60. https://doi.org/10.1016/j.mycres.2008.11.003
  9. Petrini O. Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS, editors. Microbial ecology of leaves. New York: Springer-Verlag; 1991. p. 179-97.
  10. Stone JK, Sherwood MA, Carroll GC. Canopy microfungi: function and diversity. Northwest Sci 1996;70:37-45.
  11. Ganley RJ, Newcombe G. Fungal endophytes in seeds and needles of Pinus monticola. Mycol Res 2006;110(Pt 3):318-27. https://doi.org/10.1016/j.mycres.2005.10.005
  12. Wang Y, Guo LD. A comparative study of endophytic fungi in needles, bark, and xylem of Pinus tabulaeformis. Can J Bot 2007;85:911-7. https://doi.org/10.1139/B07-084
  13. Arnold AE, Henk DA, Eells RL, Lutzoni F, Vilgalys R. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia 2007;99:185-206. https://doi.org/10.1080/15572536.2007.11832578
  14. Choi DS, Kayama M, Jin HO, Lee CH, Izuta T, Koike T. Growth and photosynthetic responses of two pine species (Pinus koraiensis and Pinus rigida) in a polluted industrial region in Korea. Environ Pollut 2006;139:421-32. https://doi.org/10.1016/j.envpol.2005.06.006
  15. Son Y, Hwang JW, Kim ZS, Lee WK, Kim JS. Allometry and biomass of Korean pine (Pinus koraiensis) in central Korea. Bioresour Technol 2001;78:251-5. https://doi.org/10.1016/S0960-8524(01)00012-8
  16. Paul NC, Kim WK, Woo SK, Park MS, Yu SH. Fungal endophytes in roots of Aralia species and their antifungal activity. Plant Pathol J 2007;23:287-94. https://doi.org/10.5423/PPJ.2007.23.4.287
  17. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  18. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30.
  19. Chun J. Computer-assisted classification and identification of actinomycetes [dissertation]. Newcastle Upon Tyne: University of Newcastle; 1995.
  20. Swofford DL. PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland: Sinauer Associates; 2002.
  21. Rayner RW. A mycological colour chart. Kew, Surrey: Commonwealth Mycological Institute and British Mycological Society; 1970.
  22. Sullivan RF, White JF Jr. Phoma glomerata as a mycoparasite of powdery mildew. Appl Environ Microbiol 2000;66:425-7. https://doi.org/10.1128/AEM.66.1.425-427.2000