International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Alteration of LAR-RPTP Expression in the Rat Trigeminal Ganglion after Tooth Extraction

  • Kim, Sun-Hun (Dental Science Research Institute, Chonnam National University) ;
  • Kim, Hyun-Jin (Institute of Biomaterial Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University)
  • Received : 2011.10.10
  • Accepted : 2011.11.29
  • Published : 2011.12.30
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Abstract

LAR-RPTP (leukocyte common antigen-related receptor protein tyrosine phosphatase) is an important regulator in the nervous system, but little is known about its expression pattern in rat trigeminal ganglion (TG) neurons. To examine whether LAR-RPTP is expressed in the TG in the current study, we sacrificed rats at 0, 7, 10 and 56 day postpartum (dpp) and a second group of rats at 3 and 5 days after an experimental tooth extraction as a TG injury model. RT-PCR was then used to determine the level of LAR-RPTP expression in the TG and immunohistology was employed to detect the subcellular localization of the protein. The mRNA expression of LAR-RPTP during the developmental stages in the TG was found to gradually increase. After experimental tooth extraction however, these transcript levels had significantly decreased at three days. LAR-RPTP protein signals in the TG were found to be cytoplasmic in the normal animals but interestingly, at five days after an experimental tooth extraction, these signals were rare. These results indicate that LAR-RPTP may be regulated during both the developmental as well as regenerative processes that take place in the TG. This further suggests that LAR-RPTP is not only involved in primary axonogenesis but possibly also in the molecular control of axons during TG repair.

Keywords

References

  1. Andersen JN, Mortensen OH, Peters GH, Drake PG, Iversen LF, Olsen OH, Jansen PG, Andersen HS, Tonks NK, Møller NP. Structural and evolutionary relationships among protein tyrosine phosphatase domains. Mol Cell Biol. 2001;21:7117- 7136. https://doi.org/10.1128/MCB.21.21.7117-7136.2001
  2. Bixby JL. Receptor tyrosine phosphatases in axon growth and guidance. NeuroReport. 2000;11:R5-R10. https://doi.org/10.1097/00001756-200007140-00001
  3. Desai CJ, Popova E, Zinn K. A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP"carbohydrate epitope. J Neurosci. 1994;14:7272-7283.
  4. Fried K, Arvidsson J, Robertson B, Pfaller K. Anterograde horseradish peroxidase tracing and immunohistochemistry of trigeminal ganglion tooth pulp neurons after dental nerve lesions in the rat, Neuroscience. 1991;43:269-278. https://doi.org/10.1016/0306-4522(91)90434-P
  5. Fried K, Bongenhielm U, Boissonade FM, Robinson PP. Nerve injury-induced pain in the trigeminal system. Neuroscientist. 2001;7(2):155-165. https://doi.org/10.1177/107385840100700210
  6. Ryu HC, Piao ZG, Choi SY, Lee SJ, Park KP, Kim JS, Oh SB. GABAergic synaptic input to masencephalic trigeminal neurons in rat. Int J Oral Biol. 2005;30(2):71-76.
  7. Haworth K, Shu KK, Stokes A, Morris R, Stoker A. The expression of receptor tyrosine phosphatases is responsive to sciatic nerve crush. Mol Cell Neurosci. 1998;12(3):93-104. https://doi.org/10.1006/mcne.1998.0707
  8. Marfurt CF. The somatotopic organization of the cat trigeminal ganglion as determined by the horseradish peroxidase technique. Anat Rec. 1981;201:105-118. https://doi.org/10.1002/ar.1092010113
  9. McLean J, Batt J, Doering LC, Rotin D, Bain JR. Enhanced rate of nerve regeneration and directional errors after sciatic nerve injury in receptor protein tyrosine phosphatase sigma knock-out mice. J Neurosci. 2002;22:5481-5491.
  10. Mizuno K, Hasegawa K, Ogimoto M, Katagiri T, Yakura H. Developmental regulation of gene expression for the MPTP delta isoforms in the central nervous system and the immune system. FEBS Lett. 1994;355:223-228. https://doi.org/10.1016/0014-5793(94)01188-5
  11. Sim MY, Yeon KY, Choi SY, Lee SJ, Park KP, Lee JH, Oh SB, Kim JS. Modulation of calcium currents by nociceptin/ orphanin FQ in trigeminal neurons. Int J Oral Biol. 2003; 28(2):43-47.
  12. Schaapveld RQ, Schepens JT, Bachner D, Attema J,Wieringa B, Jap PH, Hendriks WJ. Developmental expression of the cell adhesion molecule-like protein tyrosine phosphatases LAR, RPTPdelta and RPTPsigma in the mouse. Mech. Dev. 1998;77:59-62. https://doi.org/10.1016/S0925-4773(98)00119-1
  13. Sessle BJ. Acute and chronic craniofacial pain:brainstem mechanisms of nociceptive transmission and neuroplasticity, and their clinical correlates. Crit Rev Oral Biol Med. 2000; 11:57-91. https://doi.org/10.1177/10454411000110010401
  14. Sommer L, Rao M, Anderson DJ. RPTP delta and the novel protein tyrosine phosphatase RPTP psi are expressed in restricted regions of the developing central nervous system. Dev Dyn. 1997;208:48-61. https://doi.org/10.1002/(SICI)1097-0177(199701)208:1<48::AID-AJA5>3.0.CO;2-1
  15. Stoker AW, Gehrig B, Haj F, Bay BH. Axonal localization of the CAM-like tyrosine phosphatase CRYP alpha:a signalling molecule of embryonic growth cones. Development. 1995; 121:1833-1844.
  16. Stoker A. Receptor tyrosine phosphatases in axon growth and guidance. Curr Opin Neurobiol. 2001;11:95-102. https://doi.org/10.1016/S0959-4388(00)00179-3
  17. Sun QL, Wang J, Bookman RJ, Bixby JL. Growth cone steering by receptor tyrosine phosphatase delta defines a distinct class of guidance cue. Mol Cell Neurosci. 2000;16:686-695. https://doi.org/10.1006/mcne.2000.0893
  18. Thompson KM, Uetani N, Manitt C, Elchebly M, Tremblay ML, Kennedy TE. Receptor protein tyrosine phosphatase sigma inhibits axonal regeneration and the rate of axon extension. Mol Cell Neurosci. 2003;23:681-692.
  19. Tian SS, Tsoulfas P, Zinn K. Three receptor-linked proteintyrosine phosphatases are selectively expressed on central nervous system axons in the Drosophila embryo. Cell. 1991; 67:675-680. https://doi.org/10.1016/0092-8674(91)90063-5
  20. Van der Zee CE, Man TY, Van Lieshout EM, Van der Heijden I, Van Bree M, Hendriks WJ. Delayed peripheral nerve regeneration and central nervous system collateral sprouting in leucocyte common antigen-related protein tyrosine phosphatase deficient mice. Eur J Neurosci. 2003;17:991-1005. https://doi.org/10.1046/j.1460-9568.2003.02516.x
  21. Van Lieshout EM, Van der Heijden I, Hendriks WJ, Van der Zee CE. A decrease in size and number of basal forebrain cholinergic neurons is paralleled by diminished hippocampal cholinergic innervation in mice lacking leukocyte common antigen-related protein tyrosine phosphatase activity. Neuroscience. 2001;102(4):833-841. https://doi.org/10.1016/S0306-4522(00)00526-1
  22. Van VD. Adhesion and signaling in axonal fasciculation. Curr Opin Neurobiol. 1998;8:80-86. https://doi.org/10.1016/S0959-4388(98)80011-1
  23. Wakisaka S, Takidita S, Youn SH, Kurisu K. Partial coexistence of neuropeptide Y and calbindin D28k in the trigeminal ganglion following peripheral axotomy of the inferior alveolar nerve in the rat. Brain Res 1996;707:228-234. https://doi.org/10.1016/0006-8993(95)01262-1
  24. Wang J, Bixby JL. Receptor tyrosine phosphatase-delta is a homophilic, neurite-promoting cell adhesion molecular for CNS neurons. Mol Cell Neurosci.1999;14:370-384. https://doi.org/10.1006/mcne.1999.0789
  25. Xie Y, Yeo TT, Zhang C, Yang T, Tisi MA, Massa SM, Longo FM. The leukocyte common antigen-related protein tyrosine phosphatase receptor regulates regenerative neurite outgrowth in vivo. J Neurosci. 2001;21:5130-5138.
  26. Yan H, Grossman A, Wang H, D'Eustachio P, Mossie K, Musacchio JM, Silvennoinen O, Schlessinger J. A novel receptor tyrosine phosphatase sigma that is highly expressed in the nervous system. J Biol Chem. 1993;268:24880-24886.