Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Research for the antiinflamation and antioxidation effect on the Lycoris squamigera Maxim

소염 및 항산화제 개발을 위한 상사화의 효능 연구

  • Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Sciences, Won-Kwang University) ;
  • Yoo, Myung-Ja (Division of Nanobiochemistry, College of Natural Sciences, Won-Kwang University) ;
  • Song, Mi-Seon (Division of Nanobiochemistry, College of Natural Sciences, Won-Kwang University) ;
  • Kwon, Tae-Oh (Pet Animal-Plant Science, Won-Kwang University) ;
  • Lee, Young-Hang (Division of Nanobiochemistry, College of Natural Sciences, Won-Kwang University) ;
  • Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Sciences, Won-Kwang University)
  • 김보미 (원광대학교 자연과학대학 나노생명화학부) ;
  • 유명자 (원광대학교 자연과학대학 나노생명화학부) ;
  • 송미선 (원광대학교 자연과학대학 나노생명화학부) ;
  • 권태오 (원광대학교 생명자원과학 원예 애완 동.식물 학부) ;
  • 이영행 (원광대학교 자연과학대학 나노생명화학부) ;
  • 채규윤 (원광대학교 자연과학대학 나노생명화학부)
  • Received : 2011.11.18
  • Accepted : 2011.12.21
  • Published : 2011.12.30
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Abstract

Objectives : The Lycoris squamigera Maxim has been used traditionally for treatment of various diseases. However, the studies on the effect of Lycoris squamigera Maxim have not been carried out. In the present study, extract of Lycoris squamigera Maxim were tested for their anti-inflammatory and anti-oxidation effect. Methods : The anti-inflammatory effect of the various solvent extract was studied in lipopolysaccharide (lps)-treated mouse macrophage cells. RAW 264.7 cells were pre-incubated with Lycoris squamigera Maxim extracts for 4h and treated with $1\;{\mu}g/m{\ell}$ lps for 18h, and then the anti-inflammatory effects of extracts were determined. The anti-oxidation effect of extracts measured by DPPH method, reductive potential test, total phenolics test. Results : Extracted root's ethyl acetate layer showed a significant decrease in nitric oxide. And that layer (root's ethyl acetate extract) was showed decrease in TNF-${\alpha}$ concentration dependently. Separated from Root's ethyl acetate extract was fraction 1 has $0.1{\sim}5\;{\mu}M$ range, fraction 2 has $0.1{\sim}10\;{\mu}M$ range did not showed cytotoxicity. Anti-oxidation result as DPPH test showed the best was root ethyl acetate extract. Redusing power was made a comparison between fractions and standard. They were showed similar value. Fraction's total phenol containing result was better then standard. Conclusions : These results suggest that these extracts can be used as anti-inflammatory, anti-axidation materials.

Keywords

References

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