Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Monitoring on Microbial flora of Herbal Powder in Long Term Preservation

장기 보존 한약 파우더의 미생물 모니터링

  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Kwang-Soo (Dept. of Microbiology & Biotechnology, Dae-Jeon University)
  • 서창섭 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹) ;
  • 신광수 (대전대학교 자연과학대학 미생물생명공학과)
  • Received : 2011.10.28
  • Accepted : 2011.12.19
  • Published : 2011.12.30
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Abstract

Objectives : This study was carried out to moniter microbial flora on freeze-dried herbal powder and identify isolated bacteria. Methods : We measured the total number of bacteria and fungi in 29 herbal powder which had made according to the guideline of KFDA. For the identification, we observed microscopic properties and carried out polymerase chain reaction(PCR). The purified DNA was analyzed by DNA sequencer. Results : Among the 29 herbal powders, the fungi were detected only one sample as unacceptable range of total aerobic bacteria. Isolated bacteria were identified as Bacillus cereus, B. subtilis, B. megaterium, B. licheniformis, Erwinia tasmaniensis, E. amylovora, and Pantoea agglomerans by 16S rDNA analysis. E. tasmaniensis was observed 20 herbal samples. Conclusions : According to above results, further studies for the effective sterilization of low herbal materials should be needed.

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References

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