Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Hwangyeonhaedok-tang on Experimental Mouse Colitis Induced by Dextran Sulfate Sodium

황련해독탕(黃連解毒湯)이 Dextran Sulfate Sodium 유도 마우스 대장염에 미치는 영향

  • Lim, Dae-Hwan (Gam-Cho Oriental Medicine) ;
  • Yun, Ji-Yeon (Dept. of Oriental Medical Prescription, Won-Kwang University) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeon-Ju University) ;
  • Yun, Young-Gab (Dept. of Oriental Medical Prescription, Won-Kwang University)
  • 임대환 (감초한의원) ;
  • 윤지연 (원광대학교 한의과대학 방제학교실) ;
  • 장선일 (전주대학교 대체의학대학) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Received : 2011.11.07
  • Accepted : 2011.12.02
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Hwangyeonhaedok-tang(HHDT) has been traditionally used for various clinical symptoms associated with gastrointestinal disorder, cardiovascular diseases, and inflammation in the Oriental medicine. However, little is known for antioxidant and anti-inflammatory effects of HHDT on dextran-sulfate sodium(DSS)-induced colitis in mice. Methods : In this study, we investigated an antioxidant and anti-inflammatory effects of HHDT on DSS-induced colitis in mice. An experimental colitis was induced by daily treatment with 5% DSS. HHDT was orally administrated the various concentrations(25-100 mg/kg, body weight/day) for 7 days with one time per day. Results : HHDT reduced significantly clinical sign of DSS-induced colitis, including body weight loss, shorten colon length, disease activity index(DAI), and histological colon injury. HHDT also inhibited significantly serum NO and prostaglandine $E_2(PGE_2)$ productions in DSS-induced colitis mice. Furthermore, HDDT increased significantly an superoxide anion(SOD), catalase, and glutathione peroxidase(GPx) activity of the colon tissue in DSS-induced colitis mice. Conclusions : These results suggest that HHDT administration could reduce significantly the clinical signs and inflammatory mediators, and increase antioxidant activity in DSS-induced colitis model mice and is a good candidate for further evaluation as an effective anti-ulcerative agent.

Keywords

References

  1. 王燾. 外臺秘要. 北京:人民衛生出版社. 1996:72.
  2. 辛民敎. 원색 임상본초학. 남산당. 1986:310, 312, 313, 308, 309, 279.
  3. 金亨律. 東醫寶監 中 황련해독탕의 활용에 대한 硏究. 원광대학교대학원. 2007.
  4. 許浚. 對譯 東醫寶鑑. 동의보감출판사. 2006:1172, 1194, 1195.
  5. 尹用甲. 新東醫方劑 2223 도해임상활용. 鼎談. 2006:2456.
  6. 류기원, 염증성장질환. 한방내과학회지. 2005;spr(1):24-31.
  7. 한종현. 한방약리학. 서울:의성당. 2004:187-90.
  8. 孫昌鳳. 항균活性작용에 대한 황련해독탕의 효능에 관한 연구. 원광대학교. 1986.
  9. 鞠潤範. 황련해독탕이 자발적 고혈압白鼠의 혈압 및 신장기능에 미치는 영향. 원광대학교. 2001.
  10. 鄭在銀. 황련해독탕이 저산소증에 의한 배양 심근세포枯死에 미치는 영향. 원광대학교. 2004.
  11. 김대희. 황련해독탕 추출물의 항염증효과. 대구한의대. 2009.
  12. Stange EF, Travis SP, Vermeire S, Beglinger C, Kupcinkas L, Geboes K, Barakauskiene A, Villanacci V, Von Herbay A, Warren BF, Gasche C, Tilg H, Schreiber SW, Scholmerich J, Reinisch W; European Crohn's and Colitis Organisation. European evidence based consensus on the diagnosis and management of Crohn's disease: definitions and diagnosis. Gut. 2006;55 Suppl 1:i1-i15. https://doi.org/10.1136/gut.2005.081950a
  13. Zhong YQ, Huang HR, Zhu ZH, Chen QK, Zhan J, Xing LC. Effects of sulfasalazine on biopsy mucosal pathologies and histological grading of patients with active ulcerative colitis. World J Gastroenterol. 2005;11:4435-8. https://doi.org/10.3748/wjg.v11.i28.4435
  14. Jiang XL, Cui HF. An analysis of 10218 ulcerative colitis cases in China. World J Gastroenterol. 2002;8:158-61. https://doi.org/10.3748/wjg.v8.i1.158
  15. Leong RW, Lau JY, Sung JJ. The epidemiology and phenotype of Crohn's disease in the Chinese population. Infl amm Bowel Dis. 2004;10:646-51. https://doi.org/10.1097/00054725-200409000-00022
  16. Ouyang Q, Tandon R, Goh KL, Ooi CJ, Ogata H, Fiocchi C. The emergence of inflammatory bowel disease in the Asian Pacific region. Curr Opin Gastroenterol. 2005;21:408-13.
  17. Anonymous. Pharmacopoeia of the People''s Republic of China. English Edition. Guangdong Science and Technology Press. Guangzhou. 1992:82-3.
  18. Cooper HS, Murphy SN, Shah RS, Sedergran DJ. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab. Invest. 1993;69:238-49.
  19. Elson CO, Sartor RB, Tennyson GS, Riddell RH. Experimental models of inflammatory bowel disease. Gastroenterology. 1995;109:1344-67. https://doi.org/10.1016/0016-5085(95)90599-5
  20. Melgar S, Karlsson A, Michaelsson E. Acute colitis induced by dextran sulfate sodium progresses to chronicity in C57BL/6 but not in BALB/cmice: correlation between symptoms and inflammation. Am J Physiol Gastrointest Liver Physiol. 2005;288:G1328-338. https://doi.org/10.1152/ajpgi.00467.2004
  21. 안중환. 황련해독탕이 DSS로 유발된 흰쥐의 궤양성 대장염에 미치는 영향. 동국대 대학원. 2006.
  22. Buanne P, Di Carlo E, Caputi L, Brandolini L, Mosca M, Cattani F, Pellegrini L, Biordi L, Coletti G, Sorrentino C, Fedele G, Colotta F, Melillo G, Bertini R. Crucial pathophysiological role of CXCR2 in experimental ulcerative colitis in mice. J Leukoc Biol. 2007;82:1239-46. https://doi.org/10.1189/jlb.0207118
  23. Specian RD, Oliver MG. Functional biology of intestinal goblet cells. Am J Physiol. 1991 Feb;260(2 Pt 1):C183-93. https://doi.org/10.1152/ajpcell.1991.260.2.C183
  24. Weinberg JB, Misukonis MA, Shami PJ, Mason SN, Sauls DL, Dittman, WA, Wood ER, Smith GK, McDonald B, Bachus KE, et al. Human mononuclear phagocyte inducible nitric oxide synthase(iNOS): analysis of iNOS mRNA, iNOS protein, biopterin, and nitric oxide production by blood monocytes and peritoneal macrophages. Blood. 1995;86(3):1184-95.
  25. Blanco JC, Contursi C, Salkowski CA, DeWitt DL, Ozato K, Vogel SN. Interferon regulatory factor (IRF)-1 and IRF-2 regulate interferon gamma-dependent cyclooxygenase 2 expression. J. Exp. Med. 2000;191(12):2131-44. https://doi.org/10.1084/jem.191.12.2131
  26. Ko KM, Ip SP, Poon MK, Wu SS, Che CT, Ng KH, Kong YC. Effect of a lignan-enriched fructus schisandrae extract on hepatic glutathione status in rats: protection against carbon tetrachloride toxicity. Planta Med. 1995;61:134-7. https://doi.org/10.1055/s-2006-958032
  27. Simmonds NJ, Rampton DS. Inflammatory bowel disease: a radical view. Simmonds NJ, Rampton DS. Gut. 1993;34:865-8. https://doi.org/10.1136/gut.34.7.865