Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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A New HPLC-analytical Method for Total Sphingosine Contents as an Indirect Index for the Ganglioside Contents of Deer Antlers

  • Choi, Hye-Ok (Life science Research Institute, ES-Biotech. Co. Ltd.) ;
  • Kim, Jeung-Won (Life science Research Institute, ES-Biotech. Co. Ltd.) ;
  • Jo, Sung-Jun (Life science Research Institute, ES-Biotech. Co. Ltd.) ;
  • Kim, Jung-Hwan (Life science Research Institute, ES-Biotech. Co. Ltd.) ;
  • Han, Byung-Hoon (Life science Research Institute, ES-Biotech. Co. Ltd.)
  • Received : 2011.08.23
  • Accepted : 2011.11.20
  • Published : 2011.12.30
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Abstract

Routinely applicable HPLC assay procedures for the ganglioside content in various deer antler preparations were established through the creation of a UV-absorbing chromophoric substance - trans-${\alpha},{\beta}$-unsaturated-hexadecene-aldehyde - from the sphingosine moiety in ganglioside molecules by two step chemical reactions. In order to guarantee the assay's accuracy and sensitivity, the HPLC-assay procedure adopted internal reference procedures by mixing cis-${\alpha},{\beta}$-unsaturated-hexadecene aldehyde[V] or cis-3-heptadecene- 1,2-diol[IV] to assay samples. The internal reference compound [IV] or [V] was synthesized in our laboratory starting from mannitol-diacetonide through three or four step organic reactions. This new HPLC-assay procedure was successfully applied to deer antler extracts with good dose-dependent calibration curves at the picomole level of gangliosides.

Keywords

References

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