Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Application of alkaline phosphatase staining of cytology specimen for differential diagnosis of canine osteosarcoma

세포 검사시료에서 alkaline phosphatase 염색법을 활용한 개 골육종의 감별 진단

  • Park, Byoung-Yong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, Chul (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Cho, Ho-Seong (College of Veterinary Medicine and Veterinary Diagnostic Center, Chonbuk National University)
  • 박병용 (전북대학교 수의과대학 및 생체안전성연구소) ;
  • 박철 (전북대학교 수의과대학 및 생체안전성연구소) ;
  • 조호성 (전북대학교 수의과대학 및 동물질병진단센터)
  • Received : 2011.11.16
  • Accepted : 2011.11.30
  • Published : 2011.12.30
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Abstract

Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone tumor. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, malignant fibrous histiocytoma and malignant peripheral nerve sheath tumor. The purpose of this study is to introduce alkaline phosphatase (ALP) staining to differentiate OSA from other mesenchymal tumors. Tumors actively producing bone are specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 10 minutes with nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate toluidine salt-phosphatase substrate. Among 20 cases of cytology specimen, 14 were positive for ALP staining and histopathology, 6 were negative for ALP staining and histopathology. ALP staining was 100% sensitive and specificity for the diagnosis of OSA. Aspirate cytology with ALP staining was a simple, fast, safe and accurate diagnostic test for the evaluation of suspected OSA lesions in dogs.

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References

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