Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Screening on Allogeneic Mixed Lymphocyte Culture Inhibitory Activity for the Extracts of Marine-Derived Microorganisms

해양미생물 추출물의 동종세포반응 (Allogeneic Mixed Lymphocyte Culture) 억제효능 검색

  • Yun, Keum-Ja (Department of Chemistry, Pukyong National University) ;
  • Oh, Keun-Hee (Department of Biomedical Sciences/Transplantation Research Institute, Seoul National University College of Medicine) ;
  • Lee, Dong-Sup (Department of Biomedical Sciences/Transplantation Research Institute, Seoul National University College of Medicine) ;
  • Choi, Hong-Dae (Department of Chemistry, Dongeui University) ;
  • Kang, Jung-Sook (School of Dentistry, Pusan National University) ;
  • Son, Byeng-Wha (Department of Chemistry, Pukyong National University)
  • 윤금자 (부경대학교 화학과) ;
  • 오근희 (서울대학교 의과대학 의과학과/장기이식연구소) ;
  • 이동섭 (서울대학교 의과대학 의과학과/장기이식연구소) ;
  • 최홍대 (동의대학교 화학과) ;
  • 강정숙 (부산대학교 치과대학) ;
  • 손병화 (부경대학교 화학과)
  • Received : 2011.09.26
  • Accepted : 2011.11.17
  • Published : 2011.12.31
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Abstract

In order to screen new allogeneic mixed lymphocyte culture (allo-MLR) inhibitor which is expected to be immunomodulating drug lead, we have investigated allo-MLR inhibitory activity on the marine-derived symbiotic microorganisms (1,895 strains) from the marine algae. The potent inhibitory activities (over 45% inhibition of proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 46 strains. While, the significant stimulating activities (over 100% proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 5 strains. In the second assay using 46 bioactive strains, 14 strains exhibited again significant allo-MLR inhibitory activity. Finally, 11 strains among the 14 strains inhibited proliferation and IFN-${\gamma}$ production of CD4+ T cells during the stimulation with specific antigen in the third assay. On the basis of above results, the marine algae is nice source for isolation of immunomodulating microorganism, and the marine algae-associated microorganism is also nice target for development of the new immunomodulating drug lead.

Keywords

References

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