식품제조공장 내 공중부유미생물 오염도와 오염진균동정

Identification and Concentration of Airborne Microbes in Food Manufacturing Plants

  • 곽현정 (한국식품정보원 곰팡이연구센터) ;
  • 이헌준 (일본위생미생물연구센터) ;
  • 이상호 (한국식품정보원 곰팡이연구센터) ;
  • 나혜진 (한국식품정보원 곰팡이연구센터)
  • Gwak, Hyun-Jung (Fungi Research Center, Korea Food information Institute) ;
  • Lee, Hun-June (Hygiene and Microbiology Research Center) ;
  • Lee, Sang-Ho (Fungi Research Center, Korea Food information Institute) ;
  • Na, Hye-Jin (Fungi Research Center, Korea Food information Institute)
  • 투고 : 2011.04.06
  • 심사 : 2011.09.29
  • 발행 : 2011.12.31

초록

To evaluate the indoor air quality of food manufacturing plants, the presence of viable bacteria and fungi was assessed in the indoor air of the facilities at which 9 food items were manufactured. Air samples were collected from the general zone, low clean zone and clean zone of each factory with an air sampler, in combination with plate counts agar using for bacteria, and dichloran-glycerol agar for fungi. The samples were incubated at $25^{\circ}C$ for 4 to 7 days. After culture, the colony forming units (CFU) on each plate were counted and corrected with a positive hole conversion table. The average concentration of bacteria was $2.2{\times}10^3\;CFU/m^3$ in the general zone, $1.2{\times}10^3\;CFU/m^3$ in the low clean zone and $7.3{\times}10^2\;CFU/m^3$ in the clean zone. The average concentration of fungal microbes was $2.5{\times}10^3\;CFU/m^3$ in the general zone, $2.6{\times}10^3\;CFU/m^3$ in the low clean zone, and $2.0{\times}10^2\;CFU/m^3$ in the clean zone. No meaningful differences were detected between the general zone and the low clean zone, but the clean zone had significantly lower concentrations than the other zones. Additionally, the identification of the fungi was performed according to morphological method using a giant culture and slide culture. The fungi were identified as belonging to 18 genera, and the genera Cladosporium(33%), Penicillium(29%) and Aspergillus(26%), predominated. Aspergillus isolates were identified to species level, and A. ochraceus, a mycotoxigenic species, was identified. As part of the effort to control the quality of the indoor air of food manufacturing plants, our results show that continued studies are clearly warranted.

키워드

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