Comparison in Antioxidant Effects of Four Citrus Fruits

감귤류 4종의 항산화 효과의 비교

  • Park, Geun-Hong (College of Veterinary Medicine Life & Environment Sciences, Chungbuk National University) ;
  • Lee, Sang-Hoon (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kim, Hyun-Young (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Jeong, Heon-Sang (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kim, Eun-young (Department of Hotel Food Service & Culinary Arts, Youngdong University) ;
  • Yun, Young-Won (College of Veterinary Medicine Life & Environment Sciences, Chungbuk National University) ;
  • Nam, Sang-Yoon (College of Veterinary Medicine Life & Environment Sciences, Chungbuk National University) ;
  • Lee, Beom-Jun (College of Veterinary Medicine Life & Environment Sciences, Chungbuk National University)
  • 박근홍 (충북대학교 수의과대학 수의학과) ;
  • 이상훈 (충북대학교농업생명환경대학 식품공학과) ;
  • 김현영 (충북대학교농업생명환경대학 식품공학과) ;
  • 정헌상 (충북대학교농업생명환경대학 식품공학과) ;
  • 김은영 (영동대학교 호텔외식조리학과) ;
  • 윤영원 (충북대학교 수의과대학 수의학과) ;
  • 남상윤 (충북대학교 수의과대학 수의학과) ;
  • 이범준 (충북대학교 수의과대학 수의학과)
  • Received : 2011.07.27
  • Accepted : 2011.11.08
  • Published : 2011.12.31

Abstract

This study was performed to investigate the antioxidant effects of 4 citrus fruits including Hallabong, Cheonhyehyang, Cheonggyeon, and Jinjihyang. In this study the citrus fruits were separated in three parts of peel, pulp segment membrane (PSM), and pulp and extracted with methanol, then concentrated using a rotary vacuum evaporator. Total polyphenol contents ranged 23.497~42.341 mg/g in peel, 13.285~21.872 mg/g in PSM and 6.333~11.627 mg/g in pulp of the citrus fruits. The total polyphenol contents were highest in the peel and PSM of Jinjihyang and in the pulp of Cheonhyehyang. 1,1-diphenyl-2picryl hydrazyl (DPPH) radical scavenging activities were highest in the peel and PSM of Jinjihyang and in the pulp of Cheonhyehyang. 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacities were also highest in the peel and PSM of Jinjihyang and in the pulp of Cheonhyehyang. Reducing powers were highest in the peel of Cheonggyeon, in the PSM of Jinjihyang, and in the pulp of Cheonhyehyang. There were significant correlations in between total polyphenol contents and the radical scavenging activities, and reducing power each other. These results indicate that the 4 citrus fruits (Hallabong, Cheonhyehyang, Cheonggyeon, and Jinjihyang) evidently have antioxidant capacities and their peel parts have the highest antioxidant activities.

본 연구는 감귤 4품종의 항산화효과를 알아보기 위하여 수행되었다. 감귤 4품종은 '한라봉', '천혜향', '청견', '진지향'이다. 실험에 사용된 감귤은 과피, 양낭막, 과육 3 부분으로 분리시켰으며, 메탄올을 사용하여 추출한 후 감압 농축시켰다. 감귤 과피의 총 폴리페놀 함량은 23.497~42.341 mg/g, 양낭막에서 13.285~21.872 mg/g 그리고 과육에서 6.333~11.627 mg/g을 나타냈다. 이 중에서 과피와 양낭막 에서는 진지향이 가장 높았고, 과육에서는 천혜향이 가장 높은 총 폴리페놀 함량을 보여주었다. DPPH 라디칼 소거능 측정 결과 과피와 과육에서 진지향이 가장 높았고, 과육에서는 천혜향이 가장 높았다. ABTS 라디칼 소거능 측정에 있어서도 역사 과피와 과육에서는 진지향이 가장 높았고, 과육에서는 천혜향이 가장 높았다. 환원력에 있어서 는 청견이 과피에서 가장 높았고, 양낭막에서는 진지향 그 리고 과육에서는 천혜향이 가장 높았다. 실험 결과, 폴리페놀 함량과 라디칼 소거능 그리고 환원력 간에는 유의한 상관관계가 나타났다. 이러한 결과들은 감귤 4 품종(한라봉, 천혜향, 청견, 진지향)이 분명히 항산화력을 가지고 있으며, 부위 중 과피가 가장 높은 항산화력을 가지고 있음 을 보여주었다. 덧붙여 실험에 측정된 품종 중 진지향이 대체로 가장 높은 항산화력을 나타냈다.

Keywords

References

  1. Albertazzi, P., Steel, S.A., Clifford, E. and Bottazzi, M.: Attitude towards and use of dietary supplementation in a sample of post-menopausal women. Climacteric., 5, 374-382 (2002).
  2. Fang, Y.Z., Yang, S. and Wu, G.: Free radical antioxidant and nutrition. Nutrition, 18, 872-879 (2002) https://doi.org/10.1016/S0899-9007(02)00916-4
  3. Shin, E.H.: Compound analysis and antioxidant activity of Pueraria flos. Kor. J. Soc. Food Sci. Nutr., 38, 1139-1144 (2009). https://doi.org/10.3746/jkfn.2009.38.9.1139
  4. Kim, J.H., Park, J.H., Park, S.D., Choi, S.Y., Seong, J.H. and Moon, K.D.: Preparation and antioxidant activity of health drink with extract powders from safflower seed. Kor. J. Food Sci. Technol., 34, 617-624 (2002).
  5. Evance, C.R., Halliwell, B. and Lunt, G.G.: Free radicals and oxidative stress : environment, drugs and food additives. Portland press Inc., Seattle, pp. 1-31 (1995).
  6. El-Alfy, A.T., Ahmed, A.A.E. and Fatani, A.J.: Protective effect of red seeds proanthocyanidins against induction of diabetes by alloxan in rats. Pharmacol. Res., 52, 264-270 (2005). https://doi.org/10.1016/j.phrs.2005.04.003
  7. Joint FAO/WHO Expert committee on food additive (1968).
  8. Gilbert, D. and Golerg, L.: Liver Response Test. III. Liver enlargement and stimulation of microsome processing enzyme activity. Food Cosmet, Toxicol., 3, 417-432 (1965).
  9. Omaye, S.T., Reddy, K.A. and Cross, C.E.: Effect of butylated hydroxytoluene and other antioxidants on mouse lung metabolism. J. Toxicol. Environ. Health, 3, 829-836 (1977). https://doi.org/10.1080/15287397709529617
  10. Branen, A.L.: Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc., 52, 59-63 (1975). https://doi.org/10.1007/BF02901825
  11. Choe, S.Y. and Yang, K.H.: Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxy anisole (BHA). Kor. J. Food Sci. Technol., 14, 283-288 (1982).
  12. Durkee, A.B. and Thivierge, F.A.: Ferulic acid and other phenolics in oat seeds (Avena sativa L. var Hinoat). J. Food Sci., 42, 551-552 (1977). https://doi.org/10.1111/j.1365-2621.1977.tb01547.x
  13. Lee, S.H., Park, H.J., Back, O.H., Chun, H.K., Rhie, S.G. and Lee, G.S.: Comparison of the nutritional composition of 3 kinds of citrus produced on Jeju Island, Korea. Kor. J. Commun. Living Sci., 16, 15-20 (2005).
  14. Laura, B.: Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev., 56, 317-333 (1998).
  15. Kamiya, S. and Esaki, S.: Recent advances in the chemistry of the citrus flavonoids. Nippon Shokuhin Kogyo Gakkaishi, 18, 38-48 (1971). https://doi.org/10.3136/nskkk1962.18.38
  16. Monforte, M.T., Trovato, A., Kirjavainen, S., Forestieri, A.M., Galati, E.M. and Lo Curto, R.B.: Biological effects of hesperidin a citrus flavonoid hypolipidemic activity on experimental hypercholesterolemia in rat. Farmacology, 50, 595-599 (1995).
  17. Yoo, K.M., Park, J.B., Seoung, K.S., Kim, D.Y. and Hwang, I.K.: Antioxidant activities and anticancer effects of Yuza. Food Sci. Industry., 38, 72-77 (2005).
  18. Dewnato, V., Xianzhong, W. and Liu, R.H.: Processed sweet corn has higher antioxidant activity. J. Agric. Food Chem., 50, 4959-4964 (2002). https://doi.org/10.1021/jf0255937
  19. Blois, M.S.: Antioxidant determinations by the use a stable free radical. Nature, 26, 1199-1200 (1958).
  20. Choi, Y., Lee, S.M., Chun, J., Lee, H.B. and Lee, J.: Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem., 99, 381-387 (2006). https://doi.org/10.1016/j.foodchem.2005.08.004
  21. Mau, J.L., Lin, H.C. and Song, S.F.: Antioxidant properties of several specialty mushroom. Food Res. Int., 35, 519-526 (2002). https://doi.org/10.1016/S0963-9969(01)00150-8
  22. Kim, W.K., Kim, H.Y., Kim, M.J. and Kim, S.H.: Effects of vitamine E supplementation on antioxidant status and immune response in female athletes. Kor. J. Nutr., 32, 781-786 (1999).
  23. Kim, N.E. and Kim, W.K.: Effect of antioxidant vitamins supplementation of antioxidative status and plasma lipid profiles in Korea NIDDM patient. Kor. J. Nutr., 32, 775-780 (1999).
  24. Terao, J.: Antioxidant activity of beta-carotene-related carotenoids in solution. Lipids, 24, 659-661 (1989). https://doi.org/10.1007/BF02535085
  25. Kim, J.H. and Kim, M.K.: Effect of different of mandarin intake on antioxidative capacity in 15-month-old rats. Kor. J. Nutr., 36, 559-569 (2003).
  26. Ahn, M.S., Kim, H.J. and Seo, M.S.: A study on the antioxidative and antimicrobial activities of the Citrus unshiu peel extracts. Kor. J. Food Cult., 22, 454-461 (2007).
  27. Lee, G.D., Chang, H.K. and Kim, H.K.: Antioxidative and nitrite scavenging activities of edible mushrooms. Kor. J. Food Sci. Technol., 29, 432-436 (1997).
  28. Anagnostopoulou, M.A., Kefalas, P, Papageorgiou, V.P., Assimopoulou, A.N. and Boskou, D.: Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem., 94, 19-25 (2006). https://doi.org/10.1016/j.foodchem.2004.09.047
  29. Jayaprakasha, G.K., Patil, B.S.: In vitro evaluation of the antioxidant activities in fruit extracts from citron and blood orange. Food Chem., 101, 410-418 (2007). https://doi.org/10.1016/j.foodchem.2005.12.038
  30. Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N. and Suda, I.: Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars. J. Food Sci., 67, 1752-1756 (2002). https://doi.org/10.1111/j.1365-2621.2002.tb08718.x
  31. Yoo, K.M. and Hwang, I.K.: In vitro effect of yuza (Citrus junos Sieb ex Tanaka) extracts on proliferation of human prostate cancer cells and antioxidant activity. Kor. J. Food Sci. Technol., 36, 339-344 (2004).
  32. Tanaka, M., Kuie, C.W., NAgashima, Y. and Taguchi, T.: Application of antioxidative maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi, 54, 1409-1414 (1988). https://doi.org/10.2331/suisan.54.1409
  33. Meir, S., Kanner, J., Akiri, B. and Hadas, S.P.: Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agric. Food Chem., 43, 1813-1819 (1995). https://doi.org/10.1021/jf00055a012
  34. Lee, Y.J., Kim, S.I. and Han, Y.S.: Antioxidant activity and quality characteristics of yogurt added yuza (Citrus junos Sieb ex Tanaka) extract. Kor. J. Food & Nutr., 21, 135-142 (2008).