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The Korean Society of Phycology (한국조류학회(藻類))
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Comparative proteomics of the mixotrophic dinoflagellate Prorocentrum micans growing in different trophic modes

  • Shim, Jun-Bo (Department of Biology, Kongju National University) ;
  • Klochkova, Tatyana A. (Department of Biology, Kongju National University) ;
  • Han, Jong-Won (Department of Biology, Kongju National University) ;
  • Kim, Gwang-Hoon (Department of Biology, Kongju National University) ;
  • Yoo, Yeong-Du (School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University) ;
  • Jeong, Hae-Jin (School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University)
  • Received : 2011.01.23
  • Accepted : 2011.02.24
  • Published : 2011.03.15
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Abstract

Protein profiles of a common mixotrophic dinoflagellate, Prorocentrum micans, growing autotrophically and mixotrophically (fed on the cryptophyte Rhodomonas salina) were compared using two-dimensional gel electrophoresis (2-DE) to determine if they vary in different trophic modes. Approximately 2.3% of the detected proteins were differentially expressed in the different trophic modes. Twelve proteins observed only in the mixotrophic condition had lower pI value (<5) than the fifteen proteins observed only in the autotrophic condition (>5). When the internal amino acid sequences of five selected proteins differentially expressed between autotrophic and mixotrophic conditions were analyzed using matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, two proteins that were specifically expressed in the autotrophic condition showed homology to glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) and a bacterial catalase. Three mixotrophy-specific proteins showed homology to certain hypothetical proteins from an insect and bacteria. These results suggested the presence of certain gene groups that are switched on and off according to the trophic mode of P. micans.

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References

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