The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of the Biocontrol Potential of Some Medicinal Plant Materials Alone and in Combination with Trichoderma harzianum Against Rhizoctonia solani AG 2-1

  • Lee, Hye-Min (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Khan, Zakaullah (Division of Germplasm Evaluation, National Bureau of Plant Genetic Resources) ;
  • Kim, Sang-Gyu (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Kim, Young-Ho (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University)
  • Received : 2010.12.08
  • Accepted : 2011.02.04
  • Published : 2011.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Fifty five species of medicinal plant materials were tested for their antifungal activity in vitro against Rhizoctonia solani AG 2-1 and Trichoderma harzianum to select plant species that can be used to improve the biocontrol efficacy of T. harzianum. Six species were effective against R. solani AG 2-1 but were also antagonistic to T. harzianum, except for Cinnamomum loureirii stem bark (CSB). CSB inhibited mycelial growth of R. solani AG 2-1 by 73.7% but showed an inhibitory effect on mycelial growth of T. harzianum by only 2.2%. Scanning electron microscophs showed that the CSB treatment resulted in deformed R. solani AG 2-1 hyphal cells, and transmission electron microscophs revealed degenerated cell structures such as degenerated cytoplasm and disentangled cell wall and the accumulation of electron-dense inclusions (asterisks) in the CSB treatment. The biocontrol efficacy of radish damping-off increased greatly following the combined treatments of T. harzianum and CSB and the combined treatment increased efficacy from 6.4-23.1% to 37.1-87.3% compared with either treatment alone. CSB did not affect T. harzianum population growth, as it was almost the same in rice-bran peat medium (culture) amended with 0.1% and 1.0% CSB powder as in non-amended medium. The formulation of T. harzianum in rice-bran peat medium amended with CSB powder reduced the severity of radish damping-off by 80.6%, suggesting that T. harzianum and CSB can be formulated as a biocontrol product for the control of R. solani AG 2-1.

Keywords

References

  1. Bardin, S. D., Huang, H. C. and Koyer, J. R. 2003. Control of Pythium damping off of sugar beet by seed treatment with crop straw powders and a biocontrol agent. Biol. Contr. 4:1-8.
  2. Berta, G., Sampo, S., Gamalero, E., Massa, N. and Lemanceau, P. 2005. Suppression of Rhizoctonia root-rot of tomato by Glomus mossae BEG12 and Pseudomonas fluorescens A6RI is associated with their effect on the pathogen growth and on the root morphogenesis. Eur. J. Plant Pathol. 111:279-288. https://doi.org/10.1007/s10658-004-4585-7
  3. Brannen, P. M. and Kenney, D. S. 1997. Kodiak-a successful biological control product for suppression of soil-borne plant pathogens of cotton. J. Industr. Microbiol. Biotechnol. 19:169-171. https://doi.org/10.1038/sj.jim.2900439
  4. Cheng, S. S., Liu, J. Y., Hsui, Y. R. and Chang, S. T. 2006. Chemical polymorphism and antifungal activity of essential oils from leaves of different provenances of indigenous cinnamon (Cinnamomum osmophloeum). Biores. Technol. 97:306-312. https://doi.org/10.1016/j.biortech.2005.02.030
  5. Chet, I. 1987. Trichoderma: Application, mode of action and potential as a biocontrol agent of soilborne plant pathogenic fungi. In: Innovative approaches to plant disease control, ed. by I. Chet, pp. 137-160. Wiley & Sons, New York, USA.
  6. Duffy, B. 2000. Combinations of pencycuron and Pseudomonas fluorescens strain 2-79 for integrated control of Rhizoctonia root rot and take all of spring wheat. Crop Prot. 19:21-25. https://doi.org/10.1016/S0261-2194(99)00078-2
  7. Elad, Y., Chet, I. and Henis, Y. 1981. Biological control of Rhizoctonia solani in strawberry fields by Trichoderma harzianum. Plant Soil 60:245-254. https://doi.org/10.1007/BF02374109
  8. Gerhardson, B. 2002. Biological substitute for pesticides. Trends Biotechnol. 20:338-343. https://doi.org/10.1016/S0167-7799(02)02021-8
  9. Guetsky, R., Shtienberg, D., Elad, Y. and Dinnor, A. 2001. Combining biocontrol agents to reduce the variability of biological control. Phytopathology 91:621-627. https://doi.org/10.1094/PHYTO.2001.91.7.621
  10. Guetsky, R., Shtienberg, D., Elad, Y., Fischer, E. and Dinnor, A. 2002. Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology 92:976-985. https://doi.org/10.1094/PHYTO.2002.92.9.976
  11. Harman, G. E. 2000. Myths and dogmas of biocontrol: Changes in oriented derived from research on Trichoderma harzianum T- 22. Plant Dis. 84:377-393. https://doi.org/10.1094/PDIS.2000.84.4.377
  12. Hjelord, L. and Tronsmo, A. 1998. Trichoderma and Gliocladium in biological control: An overview. In: Trichoderma and Gliocladium, Vol. 2. Enzymes, biological control and commercial application, ed. by G.E. Harman and C.P. Hubicek, pp. 129-151. Taylor and Francis Ltd., London, UK.
  13. Howell, C. R. 1998. The role of antibiosis in biocontrol. In: Trichoderma and Gliocladium, Vol. 2. Enzymes, biological control and commercial application, ed. by G.E. Harman and C.P. Hubicek, pp. 173-183. Taylor and Francis Ltd., London, UK.
  14. Howell, C. R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant disease: The history and evolution of current concepts. Plant Dis. 87:4-10. https://doi.org/10.1094/PDIS.2003.87.1.4
  15. Jacob, I., Ana, M. and Ilan, C. 1996. Hyphal interaction between Trichoderma harzianum and Sclerotinia sclerotiorum and its role in biological control. Soil Biol. Biochem. 28:757-763. https://doi.org/10.1016/0038-0717(96)00010-7
  16. Kim, E. H. 2002. Acaricidal activity of phenylpropenes identified from essential oil of Eugenia caryophyllata against Dermatophagoides farinae, Dermatophagoides pteronyssinus (Acari; Pyroglyphidae) and Tyrophagus putrescentiae (Acari; Acaridae), Graduate School of Seoul National University, Korea.
  17. Kim, E. H., Kim, H. K. and Ahn, Y. J. 2003. Acaricidal activity of clove bud oil compounds against Dermatophagoides farina and Dermatophagoides pteronyssinus (Acari: Pyroglyphidae). J. Agric. Food Chem. 51:885-889. https://doi.org/10.1021/jf0208278
  18. Kim, H. K., Kim, K. D. and Jee, H. J. 1991. Enhanced suppression of red-pepper Phytophthora blight by combined applications of antagonist and fungicide. Korean J. Plant Pathol. 7:221-225.
  19. Kim, H. O., Park, S. W. and Park, H. D. 2004. Inactivation of Escherichia coli O157:H7 by cinnamic aldehyde purified from Cinnamomum cassia shoot. Food Microbiol. 21:105-110. https://doi.org/10.1016/S0740-0020(03)00010-8
  20. Kim, Y. H., Yu, Y. H. and Oh, S. H. 1996. Screening of antagonistic natural materials against Alternaria alternata. Korean J. Plant Pathol. 12:68-71.
  21. Lee, H. C. and Kim, I. S. 2006. Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium (pennyroyal). Korean J. Parasitiol. 44:133-138. https://doi.org/10.3347/kjp.2006.44.2.133
  22. Lee, H. C., Cheng, S. S. and Chang, S. T. 2005. Antifungal property of the essential oils and their constituents from Cinnamon osmophloeum leaf against tree pathogenic fungi. J. Sci. Food Agric. 85:2047-2053. https://doi.org/10.1002/jsfa.2216
  23. Lee, T. O. 2005. Biological control of soilborne disease using antagonistic organisms and natural materials. Graduate School of Seoul National University, Korea.
  24. Lee, T. O., Khan, Z., Kim, S. G. and Kim, Y. H. 2008. Amendment with peony root bark improves the biocontrol efficacy of Trichoderma harzianum against Rhizoctonia solani. J. Microbiol. Biotechnol. 18:1537-1543.
  25. Lewis, Y., Larkin, R. P. and Roger, D. L. 1998. A formulation of Trichoderma and Gliocladium to reduce damping off caused by Rhizoctonia solani and saprophytic growth of the pathogen in soilless mix. Plant Dis. 82:501-506. https://doi.org/10.1094/PDIS.1998.82.5.501
  26. Lima, G. and De Cicco, V. 2006. Integrated strategies to enhance biological control of postharvest diseases. In: Advances in post-harvest technology for horticultural crops, Vol. 1, ed. by N. Benkeblia and N. Shiomi, pp. 173-194. Research Signpost, Kerala, India.
  27. Lis-Balchin, M. and Deans, S. G. 1997. Bioactivity of selected plant essential oils against Listerria monocytogenes. J. Appl. Bacteriol. 82:759-762. https://doi.org/10.1046/j.1365-2672.1997.00153.x
  28. Lopez, P., Sanchez, C., Batlle, R. and Nerin, C. 2005. Solid- and vapor-phase antimicrobial activities of six essential oils: Susceptibility of selected foodborne bacterial and fungal strains. J. Agric. Food Chem. 53:6939-6946. https://doi.org/10.1021/jf050709v
  29. Nandakumar, R., Babu, S., Viswanathan, R., Sheela, J., Raguchander, T. and Samiyappan, R. 2001. A bioformulation containing plant growth promoting rhizobacterial mixtures for the management of sheath blight and enhanced grain yield in rice. Biocontrol 46:493-510. https://doi.org/10.1023/A:1014131131808
  30. Papavizas, G. C. 1985. Trichoderma and Gliocladium: Biology, ecology and potential for biocontrol. Annu. Rev. Phytopathol. 23:23-54. https://doi.org/10.1146/annurev.py.23.090185.000323
  31. Paulitz, T. C. and Belanger, R. R. 2001. Biological control in greenhouse system. Annu. Rev. Phytopathol. 39:25-33.
  32. Pierson, E. A. and Weller, D. M. 1994. Use of mixtures of fluorescent pseudomonads to suppress take-all and improve the growth of wheat. Phytopathology 84:940-947. https://doi.org/10.1094/Phyto-84-940
  33. Ranasinghe, L., Jayawardena, B. and Abeywickrama, K. 2002. Fungicidal activity of essential oils of Cinnamomum zeylanicum (L.) and Syzygium aromaticum (L.) Merr et L.M. Perry against rot and anthracnose pathogens isolated from banana. Lett. Appl. Microbiol. 35:208-211. https://doi.org/10.1046/j.1472-765X.2002.01165.x
  34. Reynolds, J. E. F. 1996. Martindale-The extra pharmacopoeia, 31st edition. Royal Pharmaceutical Society of Great Britain, London, UK.
  35. Ruppel, E. G., Baker, R., Harman, G. E., Hubbard, J. P., Hecker, R. J. and Chet, I. 1983. Field tests of Trichoderma harzianum Rifai aggr. as a biocontrol agent of seedling disease in several crops and Rhizoctonia root rot of sugar beet. Crop Prot. 2:399-408. https://doi.org/10.1016/0261-2194(83)90060-1
  36. Velluti, A., Sanchis, V., Ramos, A. J., Turon, C. and Marin, S. 2004. Impact of essential oil on growth rate, zearalenone and deoxynivalenol production by Fusarium graminearum under different temperature and water activity conditions in maize grain. J. Appl. Microbiol. 96:716-724. https://doi.org/10.1111/j.1365-2672.2004.02212.x
  37. Weindling, R. 1932. Trichoderma lignorum as a parasite of other soil fungi. Phytopathology 22:837-845.
  38. Zaika, L. L. 1988. Spices and herbs: Their antimicrobial activity and its determination. J. Food Safe. 9:97-118.

Cited by

  1. Plant crude extracts and yeast as alternative to synthetic fungicide for controlling postharvest green mould on citrus fruit vol.61, pp.3, 2013, https://doi.org/10.11118/actaun201361030795
  2. Volatile Compounds and Antioxidant Capacity of the Bio-Oil Obtained by Pyrolysis of Japanese Red Pine (Pinus Densiflora Siebold and Zucc.) vol.20, pp.3, 2015, https://doi.org/10.3390/molecules20033986
  3. Control of postharvest green mold of citrus fruit with yeasts, medicinal plants, and their combination vol.79, 2013, https://doi.org/10.1016/j.postharvbio.2013.01.001