Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Photodynamic Stress-Induced Nonenzymatic Antioxidant Responses in Transgenic Rice Overexpressing 5-Aminolevulinic Acid Synthase

5-Aminolevulinic Acid Synthase를 과발현하는 형질전환 벼에서 광역학적 스트레스가 유도하는 비효소적 항산화반응

  • Jung, Sun-Yo (School of Life Sciences and Biotechnology, Kyungpook National University)
  • 정선요 (경북대학교 생명공학부)
  • Received : 2011.11.02
  • Accepted : 2011.12.05
  • Published : 2011.12.31
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Abstract

We investigated photodynamic stress-induced antioxidant responses in transgenic rice overexpressing Bradyrhizobium japonicum 5-aminolevulinic acid synthase (ALA-S) coding sequence lacking plastidal transit sequence. High light of $350{\mu}mol\;m^{-2}\;s^{-1}$ decreased the quantum yield in the transgenic lines, C4 and C5, compared to that of wild-type line. By contrast, non-photochemical quenching (NPQ) levels of C4 and C5 under high light were higher than those of the transgenic lines under low light of $150{\mu}mol\;m^{-2}\;s^{-1}$ as well as wild-type line under low and high light. Greater levels of NPQ in the transgenic lines exposed to high light were in a close correlation with increases in the xanthophyll pigment, zeaxanthin. Under high light, levels of neoxanthin, violaxanthin, lutein, and ${\beta}$-carotene in the transgenic lines were lower than those in wild-type line. Taken together, nonphotochemical energy dissipation and photoprotectant xanthophyll pigments play a critical role to deal with the severe photodynamic damage in the transgenic rice plants, although they could not overcome the photodynamic stress, leading to severe photobleaching symptoms.

색소체 transit 서열이 결여된 Bradyrhizobium japonicum ALA-S 유전자를 과발현하는 형질전환 벼의 광역학적 스트레스에 의해 유도되는 항산화반응을 조사하였다. $350{\mu}mol\;m^{-2}\;s^{-1}$ 의 높은 광 수준은 야생형 벼에 비교하였을 때 형질전환 계통인 C4와 C5의 quantum yield를 감소시켰다. 대조적으로, 높은 광수준 하에서 형질전환 계통 C4와 C5의 nonphotochemical quenching (NPQ) 수준은 야생형 계통과 낮은 광 수준 하의 형질전환 계통에 비해 높은 증가를 보여주었다. 형질전환 계통에서 높은 NPQ 수준은 xanthophyll인 zeaxanthin 수준의 증가와 밀접한 관련이 있었다. $150{\mu}mol\;m^{-2}\;s^{-1}$ 의 낮은 광 수준과 비교하였을 때 높은 광 수준에서 violaxanthin 수준이 야생형 벼에서 증가하였으나, 형질전환 C4와 C5 계통에서는 현저하게 감소하였다. 형질전환 벼에서 nonphotochemical energy dissipation과 광보호기작을 가진 xanthophyll 색소가 광역학적 피해를 조절하는데 결정적인 역할을 하는 것으로 생각되나, 이러한 기작이 광역학 스트레스를 극복하지는 못하였고 결과적으로 photobleaching 증상에 이르게 하였다.

Keywords

References

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