Dominant-strains Variation of Soil Microbes by Temperate Change II

온도변화에 기인한 토양세균 우점종의 변화에 관한 연구 II

  • 박갑주 (건국대학교 이과대학 생명과학부) ;
  • 이병철 (건국대학교 이과대학 생명과학부) ;
  • 김수영 (건국대학교 이과대학 생명과학부) ;
  • 박찬선 (국립목포대학교 해양자원학과) ;
  • 조명환 (건국대학교 이과대학 생명과학부)
  • Received : 2011.07.08
  • Accepted : 2011.08.12
  • Published : 2011.08.31

Abstract

Today, the weather is changing continually, due to the progress of global warming. As the weather changes, the habitats of different organisms will change as well. It cannot be predicted whether or not the weather will change with each passing day. In particular, the biological distribution of the areas climate change affects constitutes a major factor in determining the natural state of indigenous plants; additionally, plants are constantly exposed to rhizobacteria, which are bound to be sensitive to these changes. Interest has grown in the relationship between plants and rhizopheric microorganisms. As a result of this interest we elected to research and experiment further. We researched the dominant changes that occur between plants and rhizospheric organisms due to global warming. First, we used temperature as a variable. We employed four different temperatures and four different sites: room temperature ($27^{\circ}C$), $+2^{\circ}C$, $+4^{\circ}C$, and $+6^{\circ}C$. The four different sites we used were populated by the following strains: Pinus densiflora, Pinus koraiensis, Quercus acutissima. We counted colonies of these plants and divided them. Then, using 16S rRNA analysis we identified the microorganisms. In conclusion, we identified the following genera, which were as follows: 24 strains of Bacillus, 6 Paenibacillus strains, 1 Pseudomonas strains. Among these genera, the dominant strains in Pinus densiflora was discovered in the same genus. Additionally, those of Pinus koraiensis and Quercus acutissima changed in both genus and strains which changed into the Bacillus genus from the Paenibacillus genus at $33^{\circ}C$.

Keywords

References

  1. 김윤식, 고성철, 오병운. 1981. 한국식물의 분포도에 관한 연구(V). 참나무과의 분포도. 고려대학교 이론공집. pp. 93-133.
  2. 산림청. 1999. 산림입지조사요령. pp.24-25.
  3. 임업연구원. 1999. 소나무 소나무림. 임업연구원. pp.192-203.
  4. 좌재호, 임한철, 한승갑, 전승종, 서장선. 2007. 감귤원 토양에서 분리한 인산염 가용화 미생물 Bacillus sphaericus PBS-13의 특성. 한국토양비료학회지. 40:405-411.
  5. 정태현, 이우철. 1965. 한국삼림식물대 및 적지적수론. 성균관대학교논문집. 10:329-435.
  6. Amin El, HS Hanson, B Petterson, B Petrini and LV Von Stedingk. 2000. Identification of non-tuberculous mycobacteria. 16S rRNA gene sequence analysis vs. conventional methods. Scand. J. Infect. Dis. 32:47-50. https://doi.org/10.1080/00365540050164218
  7. Caroline C. 1994. Field studies on two rock phosphate solubilizing actinomycete isolates as biofertilizer sources. Environmental Management 18:263-269. https://doi.org/10.1007/BF02393767
  8. Choi MY. 2003. Microbial Diversity on an Industrialized and Agricultural District. Department of Biotechnology and Chemical Engineering. Graduate School Yosu National University. pp. 2-3.
  9. Davison J. 1988. Plant beneficial bacteria. Biotechnology 6:282-286. https://doi.org/10.1038/nbt0388-282
  10. Houghton J. 2007. Global Warming. Hanul Academy. pp. 236-238.
  11. Illmer P and F Schinner. 1995. Solubilization of inorganic calcium phosphates solubilization mechanisms. Soil Biol. Biochem. 27:257-263. https://doi.org/10.1016/0038-0717(94)00190-C
  12. Kim HR. 1994. Antifungal antibiotic of antagonistic bacterium Bacillus sp. YH-16 against Fusarium solani causing plant root-rot. Department of applied microbiology. Graduate school. Yeungnam University. 26:86-87.
  13. Kim KK, JG Kang, SS Moon and KY Kang. 2000. Isolation and identification of antifungal N-butylbenzensulphonamide produced by Pseudomonas sp. AB2. J. Antibiotics. 53:131-136. https://doi.org/10.7164/antibiotics.53.131
  14. Kim YS and SD Kim. 1994. Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biocontrol agent. J. Microbiol. Biotech. 4:296-304.
  15. Lee EJ, KS Kim, SH Hong and JH Ha. 1995. The mechanism of biological control of Pseudomonas spp. Against Fusarium solani causing planar root-rot disease. Kor. J. Appl. Microbiol. Biotechnol. 23:91-97.
  16. Lee JM, HS Lim, TH Chang and SD Kim. 1999. Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease. Kor. J. Appl. Microbiol. Biotech. 27:427-432.
  17. Lim HS, JM Lee and SD Kim. 2002. A plant growth-promoting Pseudomonas fluorescens GL20-mechanism for disease suppression, outer membrane receptors for ferric siderophore, and genetic improvement for increased biocontrol efficacy. J. Microbiol. Biotech. 12:240-249.
  18. Yoon SY. 2008. Microbiological Diversity of Highland and Development of Biological Assessment Technology. Rural Development Administration. pp. 1-3.
  19. Xu Z, H Dai and X Li. 1986. Rational management of broadleaved Pinus Koraiensis (Korean pine) forest and improvement of woodland productivity in north-east China. The Temperate Forest Ecosystem. Institute of Terrestrial Ecology. National Environment Research Council. pp. 59-67.