Journal of Periodontal and Implant Science

  • 제41권5호
  • /
  • Pages.227-233
  • /
  • 2011
  • /
  • 2093-2278(pISSN)
  • /
  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지
DOI QR코드

DOI QR Code

Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2

  • Han, Seung-Hee (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Kim, Kyoung-Hwa (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Han, Jung-Seok (Department of Prosthodontics, Seoul National University School of Dentistry) ;
  • Koo, Ki-Tae (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Kim, Tae-Il (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Seol, Yang-Jo (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Lee, Yong-Moo (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Ku, Young (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Rhyu, In-Chul (Department of Periodontology, Seoul National University School of Dentistry)
  • 투고 : 2011.07.02
  • 심사 : 2011.08.28
  • 발행 : 2011.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.

키워드

참고문헌

  1. Schliephake H, Reiss G, Urban R, Neukam FW, Guckel S. Metal release from titanium fixtures during placement in the mandible: an experimental study. Int J Oral Maxillofac Implants 1993;8:502-11.
  2. Urban RM, Jacobs JJ, Tomlinson MJ, Gavrilovic J, Black J, Peoc'h M. Dissemination of wear particles to the liver, spleen, and abdominal lymph nodes of patients with hip or knee replacement. J Bone Joint Surg Am 2000;82:457-76. https://doi.org/10.1302/0301-620X.82B3.9310
  3. Lalor PA, Revell PA, Gray AB, Wright S, Railton GT, Freeman MA. Sensitivity to titanium. A cause of implant failure? J Bone Joint Surg Br 1991;73:25-8.
  4. Valentine-Thon E, Schiwara HW. Validity of MELISA for metal sensitivity testing. Neuro Endocrinol Lett 2003;24:57-64.
  5. Chevalier J. What future for zirconia as a biomaterial? Biomaterials 2006;27:535-43. https://doi.org/10.1016/j.biomaterials.2005.07.034
  6. Piconi C, Maccauro G. Zirconia as a ceramic biomaterial. Biomaterials 1999;20:1-25. https://doi.org/10.1016/S0142-9612(98)00010-6
  7. Akagawa Y, Hosokawa R, Sato Y, Kamayama K. Comparison between freestanding and tooth-connected partially stabilized zirconia implants after two years' function in monkeys: a clinical and histologic study. J Prosthet Dent 1998;80:551-8. https://doi.org/10.1016/S0022-3913(98)70031-9
  8. Scarano A, Di Carlo F, Quaranta M, Piattelli A. Bone response to zirconia ceramic implants: an experimental study in rabbits. J Oral Implantol 2003;29:8-12. https://doi.org/10.1563/1548-1336(2003)029<0008:BRTZCI>2.3.CO;2
  9. Kohal RJ, Weng D, Bächle M, Strub JR. Loaded custommade zirconia and titanium implants show similar osseointegration: an animal experiment. J Periodontol 2004;75:1262-8. https://doi.org/10.1902/jop.2004.75.9.1262
  10. Depprich R, Ommerborn M, Zipprich H, Naujoks C, Handschel J, Wiesmann HP, et al. Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces. Head Face Med 2008;4:29. https://doi.org/10.1186/1746-160X-4-29
  11. Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, et al. Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. Dent Mater J 2009;28:461-70. https://doi.org/10.4012/dmj.28.461
  12. Kohal RJ, Baechle M, Han JS, Hueren D, Huebner U, Butz F. In vitro reaction of human osteoblasts on alumina-toughened zirconia. Clin Oral Implants Res 2009;20:1265-71. https://doi.org/10.1111/j.1600-0501.2009.01735.x
  13. Hempel U, Hefti T, Kalbacova M, Wolf-Brandstetter C, Dieter P, Schlottig F. Response of osteoblast-like SAOS-2 cells to zirconia ceramics with different surface topographies. Clin Oral Implants Res 2010;21:174-81. https://doi.org/10.1111/j.1600-0501.2009.01797.x
  14. Suzuki A, Guicheux J, Palmer G, Miura Y, Oiso Y, Bonjour JP, et al. Evidence for a role of p38 MAP kinase in expression of alkaline phosphatase during osteoblastic cell differentiation. Bone 2002;30:91-8. https://doi.org/10.1016/S8756-3282(01)00660-3
  15. Sudo H, Kodama HA, Amagai Y, Yamamoto S, Kasai S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 1983;96:191-8. https://doi.org/10.1083/jcb.96.1.191
  16. Albrektsson T, Wennerberg A. Oral implant surfaces: Part 1--review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 2004;17:536-43.
  17. Albrektsson T, Wennerberg A. Oral implant surfaces: Part 2--review focusing on clinical knowledge of different surfaces. Int J Prosthodont 2004;17:544-64.
  18. He J, Zhou W, Zhou X, Zhong X, Zhang X, Wan P, et al. The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation. J Mater Sci Mater Med 2008;19:3465-72. https://doi.org/10.1007/s10856-008-3505-3
  19. Bachle M, Butz F, Hubner U, Bakalinis E, Kohal RJ. Behavior of CAL72 osteoblast-like cells cultured on zirconia ceramics with different surface topographies. Clin Oral Implants Res 2007;18:53-9. https://doi.org/10.1111/j.1600-0501.2006.01292.x

피인용 문헌

  1. Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways vol.31, pp.6, 2011, https://doi.org/10.3892/ijmm.2013.1346
  2. Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk vol.33, pp.2, 2011, https://doi.org/10.4012/dmj.2013-321
  3. Ginsenoside Re Promotes Osteoblast Differentiation in Mouse Osteoblast Precursor MC3T3-E1 Cells and a Zebrafish Model vol.22, pp.1, 2011, https://doi.org/10.3390/molecules22010042
  4. Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium vol.22, pp.5, 2011, https://doi.org/10.4317/medoral.22071
  5. Laser milling of yttria-stabilized zirconia by using a Q-switched Yb:YAG fiber laser: experimental analysis vol.94, pp.1, 2018, https://doi.org/10.1007/s00170-017-1020-8
  6. Ca-P spots modified zirconia by liquid precursor infiltration and the effect on osteoblast-like cell responses vol.37, pp.4, 2011, https://doi.org/10.4012/dmj.2017-246