Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Dendranthema indicum Extracts on Cell and DNA Damage Induced by Oxidative Stress

국화추출물이 산화적 스트레스에 의해 유발되는 세포와 DNA 손상에 미치는 영향

  • Park, Young-Mi (Department of Biological Science, Andong National University) ;
  • Kim, Jee-In (Department of Biological Science, Andong National University) ;
  • Lee, Chang-Ho (Gyeongbuk Institute for Bioindustry) ;
  • Lim, Jae-Hwan (Department of Biological Science, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University)
  • 박영미 (안동대학교 자연과학대학 생명과학과) ;
  • 김지인 (안동대학교 자연과학대학 생명과학과) ;
  • 이창호 (경북바이오 산업연구원) ;
  • 임재환 (안동대학교 자연과학대학 생명과학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Received : 2011.08.25
  • Accepted : 2011.12.14
  • Published : 2011.12.31
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Abstract

In this study, we evaluated the protective effects of ethanol extracts from Dendranthema indicum on cell and DNA damages induced by oxidative stress. Antioxidant activities of D. indicum extracts are higher than scavenging activities of DPPH free radical and hydroxyl radical by 92.8% and 73.8%, respectively, and higher than ferrous iron chelating effects by 59.4%. D. indicum extracts showed a protective effect on oxidative cell damage by inhibiting lipid peroxidation by 90.3% in the control group, and inhibiting expression level of p21 protein by 79.6% for the control group. This means D. indicum extracts have a great protective effect against oxidative stress. DNA fragmentation inhibition in D. indicum extracts were 89.6% for the control group, which makes the movement of DNA tail reduced, and phosphorylation of H2AX was 20.2% of the radical experiment group. This means that D. indicum extracts effectively inhibit DNA fragmentation and H2AX phosphorylation. Taken together, we suggest that ethanol extract from D. indicum has a role as a useful chemopreventor against oxidative damage.

본 연구는 국화추출물이 산화적 스트레스에 의한 세포와 DNA의 손상에 미치는 영향을 조사하였다. 국화추출물의 DPPH 유리 라디칼과 수산화 라디칼의 제거 활성은 $200{\mu}g$/ml에서 각각 92.8%, 73.8%로 나타났으며, $Fe^{2+}$-chelating 효과는 59.4%로 나타났다. 국화 추출물의 지질과산화 억제능은 대조군의 90.3%의 활성을 보였으며, p21 단백질의 발현 수준은 대조군의 79.6%로 나타나 라디칼 처리군에 비해 높은 것으로 조사되었다. 또한 국화추출물의 DNA 분절화 억제 활성은 대조군의 89.6%를 보였고, DNA tail의 이동은 농도에 따른 차이는 있으나 대조군과 비교적 유사한 수준으로 감소되는 경향을 나타내었으며 phospho-H2AX 단백질의 발현은 라디칼 처리군의 79.8%에 해당하는 수준을 보여 DNA 인산화를 억제하는 데 높은 활성을 나타내었다. 따라서 본 연구의 국화추출물은 산화적 스트레스에 의한 생체 내 독성으로부터 세포와 DNA를 보호하는 데 효과적으로 작용하는 천연 항산화 물질로서의 응용이 기대된다.

Keywords

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