생명과학회지 (Journal of Life Science)

  • 제21권2호
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  • Pages.202-210
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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지방세포분화에서의 replication factor C 단백질의 발현조절

Expressional Regulation of Replication Factor C in Adipocyte Differentiation

  • 조현국 (부산대학교 분자생물학과) ;
  • 김혜영 (부산대학교 분자생물학과) ;
  • 유현정 (부산대학교 분자생물학과) ;
  • 정재훈 (부산대학교 분자생물학과)
  • Cho, Hyun-Kook (Department of Molecular Biology, Pusan National University) ;
  • Kim, Hye-Young (Department of Molecular Biology, Pusan National University) ;
  • Yu, Hyun-Jeong (Department of Molecular Biology, Pusan National University) ;
  • Cheong, Jae-Hun (Department of Molecular Biology, Pusan National University)
  • 투고 : 2010.12.14
  • 심사 : 2010.12.15
  • 발행 : 2011.02.28
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초록

지방세포 분화 과정 중에 key regulator로서 기능을 하는 여러 전사조절인자(PPAR$\gamma$, C/EBP$\alpha$, SREBP, LXR)가 동정되었고, 주로 DNA 복제나 DNA 수선 단계에서 중요한 역할을 한다고 밝혀진 복제 조절인자인 RFC140이 지방세포 분화에도 중요한 인자임이 밝혀졌다. 이 연구에서 우리는 RFC140과 RFC38에 대한 발현조절을 확인하였으며, RFC140이 PPAR$\gamma$와의 단백질-단백질 결합을 통하여 PPAR$\gamma$에 의해 조절되는 유전자의 발현을 증가시킴을 확인하였다. 이러한 결과들은 특이적인 지방세포 전사인자에 의해 발현이 조절되는 RFC140과 RFC38이 지방세포의 분화과정에 필수적임을 제시한다.

Adipocyte differentiation is an ordered multistep process requiring the sequential activation of several groups of adipogenic transcription factors, including CCAAT/enhancer-binding protein-$\alpha$ and peroxisome proliferator-activated receptor-$\gamma$, and coactivators. In previous reports, we identified that replication factor C 140 (RFC140) protein played a critical role in regulating adipocyte differentiation as a coactivator. Here, we show expressional regulation of RFC140 and small RFC subunit, RFC38, following characterization of gene promoter of RFC140 and RFC38. In addition, RFC140 increases PPAR$\gamma$-mediated gene activation, resulting from direct protein-protein interaction of RFC140 and PPAR$\gamma$. Taken together, these findings demonstrate that the regulated expression of RFC140 and RFC38 by specific adipocyte transcription factors is required for the adipocyte differentiation process.

키워드

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