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The high dosage of earthworm (Eisenia andrei) extract decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

  • Yan, Bing-Chun (Department of Neurobiology, Kangwon National University School of Medicine) ;
  • Yoo, Ki-Yeon (Department of Oral Anatomy, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Joon-Ha (Department of Neurobiology, Kangwon National University School of Medicine) ;
  • Lee, Choong-Hyun (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Choi, Jung-Hoon (Department of Anatomy, College of Veterinary Medicine, Kangwon National University) ;
  • Won, Moo-Ho (Department of Neurobiology, Kangwon National University School of Medicine)
  • Published : 2011.09.30

Abstract

Earthworm extract has shown anticancer characteristics. In the present study, we examined the effect of chronic treatment with a high dose of earthworm (Eisenia andrei) extract (EE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of 3-week-old mice using 5-bromo-2'-deoxyuridine (BrdU) and Ki-67 immunohistochemistry for cell proliferation and doublecortin (DCX) immunohistochemistry for neuroblast differentiation, respectively. BrdU-, Ki- 67-, and DCX-immunoreactive cells were easily detected in the subgranular zone of the DG in vehicle (saline)-treated mice. However, BrdU-, Ki-67-, and DCX-immunoreactive cells in the 500 mg/kg EE-treated mice decreased distinctively compared to those in the vehicle-treated mice. In addition, brain-derived neurotrophic factor (BDNF) immunoreactivity and its protein level decreased markedly in the DG of the EE-treated group compared to those in the vehicle-treated group. These results indicate that chronic treatment with high dose EE decreased cell proliferation and neuroblast differentiation, and that BDNF immunoreactivity decreased in the DG of EE-treated mice.

Keywords

References

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