Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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The Photoautotrophic Culture System Promotes Photosynthesis and Growth of Somatic Embryo-derived Plantlets of Kalopanax septemlobus

독립영양방식 액체대량배양 시스템하에서 배양한 체세포배 유래 음나무 기내묘의 생장과 광합성

  • Park, So-Young (Division of Forest biotechnology, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Division of Forest biotechnology, Korea Forest Research Institute) ;
  • Kim, Yong-Wook (Division of Forest biotechnology, Korea Forest Research Institute)
  • 박소영 (국립산림과학원 산림생명공학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과) ;
  • 김용욱 (국립산림과학원 산림생명공학과)
  • Received : 2011.02.14
  • Accepted : 2011.03.21
  • Published : 2011.06.30
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Abstract

A photoautotrophic micropropagation methodology in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the medicinal plant Kalopanax septemlobus Nakai. Somatic embryo-derived young plantlets of K. septemlobus were cultured either under heterotrophic conditions with sucrose on half-strength MS medium with $30gL^{-1}$ sucrose, under heterotrophic conditions without sucrose, or under photoautotrophic conditions (MS liquid medium without sucrose under forced aeration) for four weeks before transferring the plantlets for acclimatization. Plantlets grown under photoautotrophic conditions had more leaves, higher chlorophyll content, a higher net photosynthetic rate (NPR), and a higher survival rate. The results indicate that the photoautotrophic conditions with a forced ventilation system are effective in enhancing the growth of plantlets and the rate of net photosynthesis. The plantlets grown under photoautotrophic conditions had a high survival rate (92%) upon ex vitro transplantation. Our study shows that autotrophically produced plantlets acclimatize better and sooner upon ex vitro transplantation than conventionally cultured plants.

당이 첨가되지 않은 액체배지를 이용한 광독립영양법은 기내배양시 대량배양에 많은 장점이 있다. 본 논문에서는 광독립영양배양 시스템을 이용하여 체세포배발생을 경유해 대량증식 된 음나무의 대량생산 시스템을 보고하고자 한다. 이를 위해 체세포배에서 발아한 음나무 기내묘는 3가지 배양 방식, 즉 $30gL^{-1}$ 당이 첨가된 1/2MS 고체배지에서 배양한 종속영양 배양(무처리구), 당이 첨가되지 않은 1/2MS 고체배지에서 배양, 그리고 당이 첨가되지 않은 1/2MS 액체배지에서 강제환기를 시켜준 독립영양 배양하에서 4주간 배양되었다. 순화전 각 배양환경하에서 자란 식물체들의 엽면적, 광합성량, 엽록소함량, 생장량 등을 조사하였다. 그 결과 독립영양하에서 생장한 유식물체의 생장이 가장 좋았으며 엽록소 함량 및 순광합성량도 대조구에 비해 월등히 증가하였다. 독립영양배양 시스템하에서 생장한 식물체들은 이후 토양에서 100% 순화되었다. 본 결과에서 배양 마지막 단계에 당이 첨가되지 않은 액체배지에 배양하는 독립영양배양 방식이 기내배양묘의 순화율을 증가시키고 기외 활착율을 높임으로서 체세포배 유래 음나무의 대량생산에 효율적인 것으로 나타났다.

Keywords

References

  1. Connolly, J.D. and Hill, R.A. 2000. Triterpenoids. Natural Product Reports 17: 463-482. https://doi.org/10.1039/b003456h
  2. Debergh, P.C. 1991. Acclimatizationtechnique of plants from in vitro. Acta Horticulturae 289: 291-300.
  3. De Riek, J., Van Clemput, O. and Debergh, P.C. 1991. Carbon metabolism of micropropagated Rosa multiflora L. In Vitro Cellular and Developmental Biology-Plant 27: 57-63. https://doi.org/10.1007/BF02632129
  4. Donnelly, D.J., Vidaver, W.E. and Lee, K.Y. 1985. The anatomy of tissue cultured red raspberry prior to an after transfer to soil. Plant Cell, Tissue and Organ Culture 4: 43-50. https://doi.org/10.1007/BF00041654
  5. Fu, L.K. 1992. China plant red data book rare and endangered plants. Vol. 1, pp. 741, Science Press, Beijing, China.
  6. Kozai, T. 1991a. Photoautotrophic miropropagation. In Vitro Cellular and Developmental Biology-Plant 27: 47-51.
  7. Kozai, T. 1991b. Controlled environment in conventional and automated micropropagation. In: I.K. Vasil (ed.), Cell Culture and Somatic Cell Genetics of Plants, pp. 213-230, Academic Press, New York, USA.
  8. Lee, S.W., Kim, Y.M., Kim, W.W. and Chung, J.M. 2002. Genetic variation of ISSR markers in the natural populations of a rare and endangered tree species, Oplopanax elatus in Korea. Journal of Korean Forest Society 91: 565-573.
  9. Lian, M.L., Murhty, H.N. and Paek, K.Y. 2002. Culture method and photosynthetic photon flux affect photosynthesis, growth and survival of Limonium 'Misty Blue' in vitro. Scientia Horticulture 95: 239-249. https://doi.org/10.1016/S0304-4238(02)00039-0
  10. Lichtenthaler, H.K. 1987. Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymology 148: 350-382.
  11. Moon, H.K., Kim, J., Park, S.Y., Kim, Y.W. and Kang, H.D. 2006. Somatic embryogenesis and plantlet formation from a rare and endangered tree species, Oplopanax elatus. Journal of Plant Biology 49: 320-325. https://doi.org/10.1007/BF03031163
  12. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth a bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Nakayama, M., Kozai, T. and Watanabe, K. 1991. Effect of the presence/absence of sugar in the medium and natural/ forced ventilation on the net photosynthetic rates of potato explants in vitro. Plant Tissue Culture Letter 8: 105-109.
  14. Pospsilova, J., Solarovam, J. and Catsky, J. 1992. Photosynthetic responses to stress during in vitro cultivation. Photosyntetica 26: 3-18.
  15. Smith, M.A.L., Palta, J.P. and McCown, B.H. 1986. Comparative anatomy and physiology of micro-cultured, seedling and greenhouse grown Asian white birch. Journal of the American Society for Horticultural Science 111: 437-442.
  16. Wang, G.S., Chen, Y.P., Xu, J.D., Murayama, T. and Shoji, J. 1996. Isolation and structure elucidation of cirenosenosides O and P from the leaves of Oplopanax elatus Nakai. Yao Xue Xue Bao 31: 940-944.
  17. Wang, G.S., Xu, J., Murayama, T. and Shoji, J. 1997. Isolationand structure elucidation of cirensenosides Q and R. Zhongoouo Zhong Yao Za Zhi 22:101-103.
  18. Wetzstein, H.Y. and Sommer, H.E. 1982. Leaf anatomy of tissue cultured Liquidambar styraciflua (Hammamelidaceae) during acclimatization. American Journal of Botany 69: 1579-1586. https://doi.org/10.2307/2442913
  19. Ziv, M. 1989. Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants. Plant Cell, Tissue and Organ Culture 17: 101-110. https://doi.org/10.1007/BF00046855
  20. Ziv, M. 1995. In vitro acclimatization. In: J. Aitken-Christie, T. Kozai, M. Lila Smith (eds.), Automation and Environmental Control in Plant Tissue Culture, P. 493-516, Kluwer Academic Publishers, Dordrecht, The Netherlands.
  21. Zobayd, S.M.A., Afreen, F., Xiao, Y. and Kozai, T. 2004. Recent advancement in research on photoautotrophic micropropagation using large culture vessels with forced ventilation. In Vitro Cellular and Developmental Biology-Plant 40: 450-458. https://doi.org/10.1079/IVP2004558