The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of modified-Okbyungpoongsan on mast cell-mediated allergic responses in RBL-2H3 mast cells

가미옥병풍산(加味玉屛風散)의 비만세포에서의 알레르기 반응에 대한 효과 연구

  • Jung, Jin-Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Park, Yong-Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University)
  • 정진기 (동국대학교 한의과대학 본초학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Received : 2011.11.08
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Objectives : In this study, we investigated the effect of modified-Okbyungpoongsan (mOP) on mast cell-mediated allergic response in basophilic leukemia cell line, RBL-2H3 mast cells. Methods : Cells were stimulated with anti-DNP-IgE after the treatment of DNP-HSA (AI/D), and then incubated with different concentrations of mOP (0.1, 0.2, 0.5 and 1 mg/$m{\ell}$) in RBL-2H3 cells. Cell toxicity was determined by WST-1 assay. The degranulation of mast cells was observed by microscope with toluidine blue staining and also the levels of beta-hexosaminidase, histamine and TNF-alpha were measured in culture supernatants by enzyme-linked immunosorbant assay. Results : mOP inhibited anti-DNP-IgE-imduced degranulation of mast cells in RBL-2H3 cells. mOP also significantly decreased the levels of histamine and inflammatory cytokine, TNF-alpha in RBL-2H3 cells, but slightly decreased the level of beta-hexosaminidase. Conclusions : These results indicate that mOP, an oriental prescription could be inhibit the allergic response through suppressing the mast cell activation.

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References

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