Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
권호 표지

Increase in concentration of soluble HLA-G in high-quality embryos after intracytoplasmic sperm injection

  • Published : 2011.12.31
⟨ Previous Next ⟩

Abstract

Non-invasive methods are normally preferred to conventional invasive methods when selecting suitable embryos to improve pregnancy rates after assisted reproduction techniques. One of the most recognized non-invasive methods is to examine the supernatants of embryo culture media. Soluble human leukocyte antigen, class I, G (sHLA-G) antigen is a non-classical class I molecule that has been widely considered as a marker of pregnancy failure or implantation success. In the current study of some Iranian patients, we examined the concentration of sHLA-G at different time points after intracytoplasmic sperm injection and compared the rates to the morphology and quality of the selected embryos. We showed that the concentration of sHLA-G increases over time in high-quality embryos. We conclude that there is a positive relationship between morphology, quality, and sHLA-G concentration. We suggest that this relationship can be used to increase the chance of a successful pregnancy.

Keywords

References

  1. Noci I, Fuzzi B, Rizzo R, Melchiorri L, Criscuoli L, Dabizzi S, Biagiotti R, Pellegrini S, Menicucci A, Baricordi OR. Embryonic soluble HLA-G as a marker of developmental potential in embryos. Hum Reprod 2005;20:138-46. https://doi.org/10.1093/humrep/deh572
  2. Lessey BA. Embryo quality and endometrial receptivity: lessons learned from the ART experience. J Assist Reprod Genet 1998;15:173-6. https://doi.org/10.1023/A:1023087900063
  3. Fisch JD, Rodriguez H, Ross R, Overby G, Sher G. The Graduated Embryo Score (GES) predicts blastocyst formation and pregnancy rate from cleavage-stage embryos. Hum Reprod 2001;16:1970-5. https://doi.org/10.1093/humrep/16.9.1970
  4. Suzumori N, Sugiura-Ogasawara M. Genetic factors as a cause of miscarriage. Curr Med Chem 2010;17:3431-7. https://doi.org/10.2174/092986710793176302
  5. Rebmann V, Switala M, Eue I, Grosse-Wilde H. Soluble HLA-G is an independent factor for the prediction of pregnancy outcome after ART: a German multi-centre study. Hum Reprod 2010;25:1691-8. https://doi.org/10.1093/humrep/deq120
  6. Ellis SA, Palmer MS, McMichael AJ. Human trophoblast and the choriocarcinoma cell line BeWo express a truncated HLA Class I molecule. J Immunol 1990;144:731-5.
  7. Paul P, Cabestre FA, Ibrahim EC, Lefebvre S, Khalil-Daher I, Vazeux G, Quiles RM, Bermond F, Dausset J, Carosella ED. Identification of HLA-G7 as a new splice variant of the HLA-G mRNA and expression of soluble HLA-G5, -G6, and -G7 transcripts in human transfected cells. Hum Immunol 2000;61:1138-49. https://doi.org/10.1016/S0198-8859(00)00197-X
  8. Rebmann V, Switala M, Eue I, Schwahn E, Merzenich M, Grosse-Wilde H. Rapid evaluation of soluble HLA-G levels in supernatants of in vitro fertilized embryos. Hum Immunol 2007;68:251-8. https://doi.org/10.1016/j.humimm.2006.11.003
  9. Sher G, Keskintepe L, Nouriani M, Roussev R, Batzofin J. Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of 'embryo competency' and IVF outcome. Reprod Biomed Online 2004;9:74-8. https://doi.org/10.1016/S1472-6483(10)62113-X
  10. Hunt JS, Petroff MG, McIntire RH, Ober C. HLA-G and immune tolerance in pregnancy. FASEB J 2005;19:681-93. https://doi.org/10.1096/fj.04-2078rev
  11. Hviid TV, Hylenius S, Lindhard A, Christiansen OB. Association between human leukocyte antigen-G genotype and success of in vitro fertilization and pregnancy outcome. Tissue Antigens 2004;64:66-9. https://doi.org/10.1111/j.1399-0039.2004.00239.x
  12. Moreau P, Paul P, Rouas-Freiss N, Kirszenbaum M, Dausset J, Carosella ED. Molecular and immunologic aspects of the nonclassical HLA class I antigen HLA-G: evidence for an important role in the maternal tolerance of the fetal allograft . Am J Reprod Immunol 1998;40:136-44. https://doi.org/10.1111/j.1600-0897.1998.tb00405.x
  13. Ng ST, Chang TH, Wu TC. Prediction of the rates of fertilization, cleavage, and pregnancy success by cumulus-coronal morphology in an in vitro fertilization program. Fertil Steril 1999;72:412-7. https://doi.org/10.1016/S0015-0282(99)00290-3
  14. Choudhury SR, Knapp LA. Human reproductive failure II: immunogenetic and interacting factors. Hum Reprod Update 2001;7:135-60. https://doi.org/10.1093/humupd/7.2.135
  15. Menicucci A, Noci I, Fuzzi B, Criscuoli L, Scarselli G, Baricordi O, Mattiuz PL. Non-classic sHLA class I in human oocyte culture medium. Hum Immunol 1999;60:1054-7. https://doi.org/10.1016/S0198-8859(99)00108-1
  16. Bamberger AM, Jenatschke S, Schulte HM, Loning T, Bamberger MC. Leukemia inhibitory factor (LIF) stimulates the human HLA-G promoter in JEG3 choriocarcinoma cells. J Clin Endocrinol Metab 2000;85:3932-6. https://doi.org/10.1210/jc.85.10.3932
  17. Margreiter M, Weghofer A, Kogosowski A, Mahmoud KZ, Feichtinger W. A prospective randomized multicenter study to evaluate the best day for embryo transfer: does the outcome justify prolonged embryo culture? J Assist Reprod Genet 2003;20:91-4. https://doi.org/10.1023/A:1021744209193
  18. Riteau B, Rouas-Freiss N, Menier C, Paul P, Dausset J, Carosella ED. HLA-G2, -G3, and -G4 isoforms expressed as nonmature cell surface glycoproteins inhibit NK and antigen-specific CTL cytolysis. J Immunol 2001;166:5018-26. https://doi.org/10.4049/jimmunol.166.8.5018
  19. Kotze DJ, Hansen P, Keskintepe L, Snowden E, Sher G, Kruger T. Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome. J Assist Reprod Genet 2010;27:309-16. https://doi.org/10.1007/s10815-010-9403-x
  20. Alinejad Z, Jafari Shakib R, Forghan-Parast K, Zahiri Z, Sadri H, Nagafi F, Roushan Z. In vitro fertilized embryos do not secrete detectable HLA-G on day two. Iran J Immunol 2009;6:195-201.