Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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NF-${\kappa}B$ and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products

  • Nam, Young-Hee (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Min, Deul-Le (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Park, Soon-Jung (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Kim, Kyeong-Ah (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Lee, Young-Ah (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Shin, Myeong-Heon (Department of Environmental Medical Biology, Yonsei University College of Medicine)
  • Received : 2011.03.18
  • Accepted : 2011.07.28
  • Published : 2011.09.30
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Abstract

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from $1{\times}10^7$ live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-${\kappa}B$ and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with $i{\kappa}B$ inhibitor or CREB inhibitor. These results suggest that transcription factors NF-${\kappa}B$ and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

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References

  1. Fouts AC, Kraus SJ. Trichomonas vaginalis: Reevaluation of its clinical presentation and laboratory diagnosis. J Infect Dis 1980; 141: 137-143. https://doi.org/10.1093/infdis/141.2.137
  2. Brill JR. Diagnosis and treatment of urethritis in men. Am Fam Physician 2010; 81: 873-878.
  3. World Health Organization. An overview of selected curable sexually transmitted diseases. Global Programme on AIDS Report, Geneva, Switzerland. 1995.
  4. Landers DV, Wiesenfeld HC, Heine RP, Krohn MA, Hillier SL. PreFig dictive value of the clinical diagnosis of lower genital tract infection in women. Am J Obstet Gynecol 2004; 190: 1004-1010. https://doi.org/10.1016/j.ajog.2004.02.015
  5. Shaio MF, Lin PR. Influence of humoral immunity on leukotriene B4 production by neutrophils in response to Trichomonas vaginalis stimulation. Parasite Immunol 1995; 17: 127-133. https://doi.org/10.1111/j.1365-3024.1995.tb01014.x
  6. Song HO, Lim YS, Moon SJ, Ahn MH, Ryu JS. Delayed human neutrophil apoptosis by Trichomonas vaginalis lysate. Korean J Parasitol 2010; 48: 1-7. https://doi.org/10.3347/kjp.2010.48.1.1
  7. Ryu JS, Kang JK, Jung SY, Shin MH, Kim JM, Park H, Min DY. Production of interleukin-8 by human neutrophils stimulated with Trichomonas vaginalis. Infect Immun 2004; 72: 1326-1332. https://doi.org/10.1128/IAI.72.3.1326-1332.2004
  8. Sun H, Chung WC, Ryu SH, Ju Z, Tran HT, Kim E, Kurie JM, Koo JS. Cyclic AMP-responsive element binding protein- and the nuclear factor-$\kappa$B-regulated CXC chemokine gene expression in lung carcinogenesis. Cancer Prev Res 2008; 1: 316-328. https://doi.org/10.1158/1940-6207.CAPR-07-0002
  9. Han IH, Goo SY, Park SJ, Hwang SJ, Kim YS, Yang MS, Ahn MH, Ryu JS. Proinflammatory cytokine and nitric oxide production by human macrophages stimulated with Trichomonas vaginalis. Korean J Parasitol 2009; 47: 205-212. https://doi.org/10.3347/kjp.2009.47.3.205
  10. Shaio MF, Lin PR, Liu JY, Yang KD. Generation of interleukin-8 from human monocytes in response to Trichomonas vaginalis stimulation. Infect Immun 1995; 63: 3864-3870.
  11. Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN. Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Infect Immun 2006; 74: 5773-5779. https://doi.org/10.1128/IAI.00631-06
  12. Kucknoor AS, Mundodi V, Alderete JF. The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65. Cell Microbiol 2007; 9: 2586-2597. https://doi.org/10.1111/j.1462-5822.2007.00979.x
  13. Shaio MF, Lin PR, Lee CS, Hou SC, Tang P, Yang KD. A novel neutrophil-activating factor released by Trichomonas vaginalis. Infect Immun 1992; 60: 4475-4482.
  14. Huang L, Zhao A, Wong F, Ayala JM, Struthers M, Ujjainwalla F, Wright SD, Springer MS, Evans J, Cui J. Leukotriene B4 strongly increases monocyte chemoattractant protein-1 in human monocytes. Arterioscler Thromb Vasc Biol 2004; 24: 1783-1788. https://doi.org/10.1161/01.ATV.0000140063.06341.09
  15. Nam YH, Min D, Kim HP, Song KJ, Kim KA, Lee YA, Kim SH, Shin MH. Leukotriene $B_4 $receptor BLT-mediated phosphorylation of NF-$\kappa$B and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products. Microbe Infect 2011 (in press).

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