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NF-${\kappa}B$ and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products

  • Nam, Young-Hee (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Min, Deul-Le (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Park, Soon-Jung (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Kim, Kyeong-Ah (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Lee, Young-Ah (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Shin, Myeong-Heon (Department of Environmental Medical Biology, Yonsei University College of Medicine)
  • Received : 2011.03.18
  • Accepted : 2011.07.28
  • Published : 2011.09.30

Abstract

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from $1{\times}10^7$ live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-${\kappa}B$ and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with $i{\kappa}B$ inhibitor or CREB inhibitor. These results suggest that transcription factors NF-${\kappa}B$ and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

Keywords

References

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