Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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In Vitro Effects of Some Herbs Used in Egyptian Traditional Medicine on Viability of Protoscolices of Hydatid Cysts

  • Yones, Doaa A. (Department of Medical Parasitology, Faculty of Medicine, Assiut University) ;
  • Taher, Gamal A. (Animal Health Research Institute, Parasitology Unit, Assiut Laboratory) ;
  • Ibraheim, Zedan Z. (Department of Pharmacognosy, Faculty of Pharmacy, Assiut University)
  • Received : 2011.05.29
  • Accepted : 2011.06.27
  • Published : 2011.09.30
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Abstract

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 ${\mu}g$/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 ${\mu}g$/ml) were used. Concentration of 2500 ${\mu}g$/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 ${\mu}g$/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 ${\mu}g$/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 ${\mu}g$/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

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References

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