Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

  • Chong, Chom-Kyu (Department of Biochemistry, College of Natural Science, Chungbuk National University) ;
  • Jeong, Woo-Seog (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service) ;
  • Kim, Hak-Yong (Department of Biochemistry, College of Natural Science, Chungbuk National University) ;
  • An, Dong-Jun (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service) ;
  • Jeoung, Hye-Young (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service) ;
  • Ryu, Jeong-Eun (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service) ;
  • Ko, A-Ra (Institute of BioMedicals, Bioland Ltd.) ;
  • Kim, Yong-Joo (Institute of BioMedicals, Bioland Ltd.) ;
  • Hong, Sung-Jong (Department of Medical Environmental Biology, College of Medicine, Chung-Ang University) ;
  • Yang, Zhaoshou (Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea) ;
  • Nam, Ho-Woo (Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea)
  • Received : 2010.04.25
  • Accepted : 2011.06.27
  • Published : 2011.09.30
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Abstract

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

Keywords

References

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