Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Simple Screening Method for Double-strand DNA Binders Using Hairpin DNA-modified Magnetic Beads

  • Jo, Hun-Ho (Department of Chemistry, Pohang University of Science and Technology) ;
  • Min, Kyoung-In (Department of Chemistry, Pohang University of Science and Technology) ;
  • Song, Kyung-Mi (Department of Chemistry, Pohang University of Science and Technology) ;
  • Ku, Ja-Kang (Department of Chemistry, Pohang University of Science and Technology) ;
  • Han, Min-Su (Department of Chemistry, Chuang-Ang University) ;
  • Ban, Chang-Ill (Department of Chemistry, Pohang University of Science and Technology)
  • Received : 2010.10.13
  • Accepted : 2010.11.16
  • Published : 2011.01.20
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Abstract

We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature ($T_m$). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs.

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References

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