Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Cimicifugae Rhizoma by HPLC

  • Fang, Zhe (College of Pharmacy, Catholic University of Daegu) ;
  • Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University) ;
  • Son, Kun-Ho (College of Life Science, Andong National University) ;
  • Son, Jong-Keun (College of Pharmacy, Yeungnam University) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu)
  • Received : 2010.07.06
  • Accepted : 2010.11.15
  • Published : 2011.01.20
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Abstract

In this study, quantitative and pattern recognition analysis for the quality evaluation of Cimicifugae Rhizoma using HPLC/UV was developed. For quantitative analysis, three major bioactive phenolic compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}M$) with isocratic elution of acetonitrile and water with 0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 323 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cimicifugae Rhizoma. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twelve reference samples corresponding to five different species of Cimicifugae Rhizoma and seventeen samples purchased from markets. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Cimicifugae Rhizoma.

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References

  1. Islam, M. N.; Lee, S. K.; Jeong, S. Y.; Kim, D. H.; Jin, C.; Yoo, H. H. Bull. Korean Chem. Soc. 2009, 30, 137. https://doi.org/10.5012/bkcs.2009.30.1.137
  2. Ajisawa, Y.; Kitazawa, M.; Uchida, M.; Kobayashi, M. Yakugaku Zasshi 1996, 116, 50. https://doi.org/10.1248/yakushi1947.116.1_50
  3. Noguchi, M.; Nagai, M.; Koeda, M.; Nakayama, S.; Sakurai, N.; Takahira M.; Kusano, G. Biol. Pharm. Bull. 1998, 21, 1163. https://doi.org/10.1248/bpb.21.1163
  4. Kruse, S. O.; Lohning, A.; Paull, G. F.; Winterhoff, H.; Nahrstedt, A. Planta Med. 1999, 65, 763. https://doi.org/10.1055/s-2006-960862
  5. Sakai, S.; Kawamata, H.; Kogure, T.; Mantani, N.; Terasawa, K.; Umatake, M.; Ochiai, H. Mediators Inflamm. 1999, 8, 173. https://doi.org/10.1080/09629359990513
  6. Watanabe, K.; Mimaki, Y.; Sakagami, H.; Sashida, Y. Chem. Pharm. Bull. 2002, 50, 121. https://doi.org/10.1248/cpb.50.121
  7. Lin, S. J.; Chen, C. S.; Lin, S. S.; Chou, M. Y.; Shih, H. C.; Lee, I. P.; Kao, C. T.; Ho, C. C.; Chen, F. L.; Ho, Y. C.; Hsieh, K. H.; Huang, C. R.; Yang, C. C. Food Chem. Toxicol. 2006, 44, 2078. https://doi.org/10.1016/j.fct.2006.07.010
  8. Qiu, M.; Kim, J. H.; Lee, H. K.; Min, B. S. Phytother. Res. 2006, 20, 945. https://doi.org/10.1002/ptr.1982
  9. Jiang, B.; Yang, H.; Nuntanakorn, P.; Balick M. J.; Kronenberg, F.; Kennelly, E. J. J. Ethnopharmacol. 2005, 96, 521. https://doi.org/10.1016/j.jep.2004.09.049
  10. Jiang, B.; Kronenberg, F.; Balick, M. J.; Kennelly, E. J. Phytomedicine 2006, 13, 477. https://doi.org/10.1016/j.phymed.2005.06.007
  11. He, K.; Pauli, G. F.; Zheng, B.; Wang, H.; Bai, N.; Peng, T.; Roller, M.; Zheng, Q. Y. J. Chromatogr. A 2006, 1112, 241. https://doi.org/10.1016/j.chroma.2006.01.004
  12. Lin, L. C.; Kuo, Y. C.; Chou C. J. J. Nat. Prod. 1999, 62, 405. https://doi.org/10.1021/np980153o
  13. Wandji, J.; Nkengfack, A. E.; Fomum, Z. T.; Ubillas, R.; Killday, K. B.; Tempesta, M. S. J. Nat. Prod. 1990, 53, 1425. https://doi.org/10.1021/np50072a004
  14. Usia, T.; Banskota, A. H.; Tezuka, Y.; Midorikawa, K.; Matsushige, K.; Kadota, S. J. Nat. Prod. 2002, 65, 673. https://doi.org/10.1021/np010486c

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