한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제28권5호
  • /
  • Pages.516-518
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  • 2011
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

정상적인 사육 황새의 장내 세균총

Bacterial Flora of the Intestine in Normal Captive Oriental White Storks

  • 한재익 (충북대학교 수의과대학) ;
  • 장혜진 (충북대학교 수의과대학) ;
  • 이숙진 (충북대학교 수의과대학) ;
  • 강효민 (충북대학교 수의과대학) ;
  • 김수경 (한국교원대학교 황새복원센터) ;
  • 박시룡 (한국교원대학교 황새복원센터) ;
  • 나기정 (충북대학교 수의과대학)
  • Han, Jae-Ik (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Jang, Hye-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Sook-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Hyo-Min (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Su-Kyung (Korea Institute of Oriental White Stork Rehabilitation Research, Korea National University of Education) ;
  • Park, Shi-Ryoung (Korea Institute of Oriental White Stork Rehabilitation Research, Korea National University of Education) ;
  • Na, Ki-Jeong (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 심사 : 2011.10.20
  • 발행 : 2011.10.31
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초록

한국 황새복원센터에서 사육중인 황새의 정상 장내 세균총을 검사하기 위한 조사를 실시하였다. 44마리의 건강한 황새의 총배설강으로부터 44개의 분변시료를 채취하여 호기 및 혐기조건에서 배양하였다. 배양된 미생물의 16Sribosomal RNA 및 heat shock protein 60 유전자의 염기서열을 조사하였다. 호기조건 하에서는 Enterococcus faecalis, Escherichia coli, Bacillus spp., Enterococcus avium, Enterococcus gallinarum, Pseudomonas spp., Alcaligenes spp., Enterobacter spp., Corynebacterium spp., Proteus mirabilis가 동정되었다. 혐기조건 하에서는 E. coli, Clostridium tertium, En. faecalis, P. mirabilis가 동정되었다. 모든 시료에서 E. coli, En. faecalis 혹은 두 가지 모두가 분리되었다. 이러한 정상 황새의 장내 미생물 정보는 향후 분변 배양 결과의 해석에 도움이 될 것으로 판단된다.

A survey was conducted to examine the normal intestinal bacterial flora of captive Oriental white storks (Ciconia boyciana) maintained at the Korea Institute of Oriental White Stork Rehabilitation Research, Cheongwon, South Korea. From the cloaca of 44 healthy storks, 44 fecal samples were collected and cultured under aerobic and anaerobic conditions. The 16S ribosomal RNA gene sequences and the heat shock protein 60 gene were cloned and sequenced for bacterial identification. Under aerobic conditions, Enterococcus faecalis, Escherichia coli, Bacillus spp., Enterococcus avium, Enterococcus gallinarum, Pseudomonas spp., Alcaligenes spp., Enterobacter spp., Corynebacterium spp., and Proteus mirabilis were identified. Under anaerobic conditions, E. coli, Clostridium tertium, En. faecalis, and P. mirabilis were identified. E. coli, En. faecalis, or both were isolated from all samples. These results will add to the information available on this stork species and help for the interpretation of fecal culture results.

키워드

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