YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Inhibitory Effect of Fermented Dioscoreae batatas Extract on Lipopolysaccharide-induced Macrophage Activation

LPS로 유도된 대식세포 활성에서 발효 산약 추출물의 억제 효과

  • Lim, Sung-Won (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lee, Sun-Hee (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Hur, Jung-Mu (Chong Kun Dang Healthcare Corp. Research Center) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Dae-Ki (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School)
  • 임성원 (전북대학교 의과대학 및 의과학연구소) ;
  • 이선희 (전북대학교 의과대학 및 의과학연구소) ;
  • 허정무 (종근당 건강(주)) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 김대기 (전북대학교 의과대학 및 의과학연구소)
  • Received : 2011.09.23
  • Accepted : 2011.09.30
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to elucidate the anti-inflammatory activities of a methanol extract derived from the fermented bark of Dioscoreae batatas on LPS-induced activation in macrophages. It was fermented with Lactobacillus fermentum and L. plantarum and then analyzed to identify the contents of methanol extract and diosgenin. The fermented product showed 3-fold increase in the extraction yield by methanol, and 1.8-fold increase in diosgenin contents, compared to that from the dried bark of D. batatas. Although the methanol extract from the unfermented D. batatas inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and TNF-${\alpha}$ in J774 A.1 cells, the methanol extract from the fermented product revealed significantly the enhanced the inhibitory activities on LPS-induced production of NO and TNF-${\alpha}$. Taken together, our results indicate that fermentation of bark of D. batatas elevates the functional activity inhibiting macrophage activation through the increase of the content of anti-inflammatory compounds. Thus, its methanol extract may be useful as a functional material for the therapy of inflammatory diseases.

Keywords

References

  1. Flora of Korea Editorial Committee : The Genera of Vascular Plants of Korea, Academy Publishing Co., Seoul. Korea. 1336 (2007).
  2. 생약학교재편찬위원회 : 생약학. 동명사. 서울. 250 (2007).
  3. Jang, S. M., Noh, S. H. and Park, S. D. : Botany of Herbal Medicine Resources. Hakmun Publishing Ltd. Seoul. Korea. 299 (1999).
  4. Yang, M. H., Yoon, K. D., Chin, Y. W. and Kim, J. W. : Phytochemical and pharmacological profiles of Dioscorea species in Korea, China and Japan. Kor. J. Pharmacogn. 40, 257 (2009).
  5. Sautour, M., Mitaine-Offer, A. C., Miyamoto, T., Wagner, H. and Lacaille-Dubois, M. A. : A new phenanthrene glycoside and other constituents from Dioscorea opposita. Chem. Pharm. Bull. 52, 1235 (2004). https://doi.org/10.1248/cpb.52.1235
  6. 하영득, 이삼빈, 곽연길 : 마 점질물의 중금속제거능과 ACE저해 효과. 한국식품영양과학회지 27, 751 (1998).
  7. Chung, Y. C., Chiang, B. H., Wei, J. H., Wang, C. K., Chen, P. C. and Hsu, C. K. : Effects of blanching, drying and extraction processes on the antioxidant activity of yam (Dioscorea alata). Int. J. Food Sci. Tech. 43, 859 (2008). https://doi.org/10.1111/j.1365-2621.2007.01528.x
  8. Jeon, J. R., Lee, J. S., Lee, C. H., Kim, J. Y., Kim, S. D. and Nam, D. H. : Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model. Arch. Pharm. Res. 29, 348 (2006). https://doi.org/10.1007/BF02968583
  9. Wang, C. H., Tsai, C. H., Lin, H. J., Wang, T. C. and Chen, H. L. : Uncooked Taiwanese yam (Dioscorea alata L. cv. Tainung No.2) beneficially modulated the large bowel function and faecal microflora in Balb/c mice. J. Sci. Food Agr. 87, 1378 (2007).729
  10. Hikino, H., Konno, C., Takahashi, M., Murakami, M., Kato, Y., Karikura, M. and Hayashi, T. : Isolation and hypoglycemic activity of dioscorans A, B, C, D, E, and F; Glycans of dioscorea japonica rhizophors1. Planta Med. 52, 168 (1986). https://doi.org/10.1055/s-2007-969112
  11. Chen, H., Wang, C., Chang, C. T. and Wang, T. : Effects of Taiwanese yam (Dioscorea japonica Thunb var. pseudojaponica Yamamoto) on upper gut function and lipid metabolism in Balb/ c mice. Nutrition. 19, 646 (2003). https://doi.org/10.1016/S0899-9007(03)00058-3
  12. Lin, C. L., Lin, S. Y., Lin, Y. H. and Hou, W. C. : Effects of tuber storage protein of yam (Dioscorea alata cv. Tainong No.1) and its peptic hydrolyzates on spontaneously hypertensive rats. J. Sci. Food Agr. 86, 1489 (2006). https://doi.org/10.1002/jsfa.2530
  13. Yang, M. H., Yoon, K. D., Chin, Y. W., Park, J. H., Kim, S. H., Kim, Y. C. and Kim, J. : Neuroprotective effects of Dioscorea opposita on scopolamine-induced memory impairment in vivo behavioral tests and in vitro assays. J. Ethnopharmacol. 121, 130 (2009). https://doi.org/10.1016/j.jep.2008.10.010
  14. Kwon, C. S., Sohn, H. Y., Kim, S. H., Kim, J. H., Son, K. H., Lee, J. S., Lim, J. K. and Kim, J. S. : Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67, 1451 (2003). https://doi.org/10.1271/bbb.67.1451
  15. Roitt, I., Brostoff, J. and Male, D. : Immunology. 3rd Ed. Mosby, New York (1993).
  16. Bosca, L., Zeini, M., Traves, P. G. and Hortelano, S. : Nitric oxide and cell viability in inflammatory cells : a role for NO in macrophage function and fate. Toxicology 208, 249 (2005). https://doi.org/10.1016/j.tox.2004.11.035
  17. Turini, M. E. and DuBois, R. N. : Cyclooxygenase-2: a therapeutic target. Annu. Rev. Med. 53, 35 (2002). https://doi.org/10.1146/annurev.med.53.082901.103952
  18. Jin, M., Suh, S. J., Yang, J. H., Lu, Y., Kim, S. J., Kwon, S., Jo, T. H., Kim, J. W., Park, Y. I., Ahn, G. W., Lee, C. K., Kim, C. H., Son, J. K., Son, K. H. and Chang, H. W. : Antiinflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-${\kappa}B$ and ERK1/2 inactivation. Food Chem. Toxicol. 48, 3073 (2010). https://doi.org/10.1016/j.fct.2010.07.048
  19. Jung, D. H., Park, H. J., Byun, H. E., Park, Y. M., Kim, T. W., Kim, B. O., Um, S. H. and Pyo, S. : Diosgenin inhibits macrophage-derived inflammatory mediators through downregulation of CK2, JNK, NF-$\kappa B$ and AP-1 activation. Int. Immunopharmacol. 10, 1047 (2010). https://doi.org/10.1016/j.intimp.2010.06.004
  20. Fuller, R. : Probiotics the scientific Basis. Chapman and Hall. London (1992).
  21. Kim, D. H., Han, S. B., Park, J. S. and Han, M. J. : Fermentation of antler and its biological activity. Kor. J. Pharmacogn. 25, 233 (1994).
  22. Chi, H. and Ji, G. E. : Transformation of ginsenosides Rb1 and Re from panax ginseng by food microorganisms. Biotechnol. Lett. 27, 765 (2005). https://doi.org/10.1007/s10529-005-5632-y
  23. Choi, Y. M., Kim, Y. S., Ra, K. S. and Suh, H. J. : Characteristics of fermentation and bioavailability of isoflavones in Korean soybean paste (doenjang) with application of Bacillus sp. KH- 15. Int. J. Food Sci. Technol. 42, 1497 (2007). https://doi.org/10.1111/j.1365-2621.2006.01371.x
  24. Cabras, P. and Angioni, A. : Pesticide residues in grapes, wine, and their processing products. J. Agric. Food Chem. 48, 967 (2000). https://doi.org/10.1021/jf990727a
  25. Isolauri, E., Salminen, S. and Ouwehand, A. C. : Probiotics. Best Prac. Res. Cl. Ga. 18, 299 (2004). https://doi.org/10.1016/j.bpg.2003.10.006
  26. Kim, J. S. and Byun, G. I. : A study on the consumers recognition, preference and use of yam and yam products focused on consumers in daegu area and andon area. Kor. J. Culinary Research 14, 441 (2008).
  27. Tal, B., Tamir, I., Rokem, J. S. and Goldberg, I. : Isolation and characterization of an intermediate steroid metabolite in diosgenin biosynthesis in suspension cultures of Dioscorea deltoidea cells. Biochem. J. 219, 619 (1984). https://doi.org/10.1042/bj2190619
  28. Wang, L. J., Wang, Y., Chen, S. W., Ma, J. S., Fu, Q. and Wang, B. X. : The antitumor activity of Diosgenin in vivo and in vitro. Zhongguo Zhong Yao Za Zhi. 27, 777 (2002).
  29. Huang, C. H., Liu, D. Z. and Jan, T. R. : Diosgenin, a plantderived sapogenin, enhances regulatory T-cell immunity in the intestine of mice with food allergy. J. Nat. Prod. 73, 1033 (2010). https://doi.org/10.1021/np900690z
  30. Stuehr, D. J. and Nathan, C. F. : Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells. J. Exp. Med. 169, 1543 (1989). https://doi.org/10.1084/jem.169.5.1543
  31. Hibbs, J. B., Jr, Taintor, R. R. and Vavrin, Z. : Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite. Science 23, 473 (1987).
  32. Bogdan, C. : Nitric oxide and the immune response. Nat. Immun. 2, 907 (2001). https://doi.org/10.1038/ni1001-907
  33. Cao, H., Urban, J. F. and Jr, Anderson, R. A. : Cinnamon polyphenol extract affects immune responses by regulating anti- and pro inflammatory and glucose transporter gene expression in mouse macrophages. J. Nutr. 138, 833 (2008). https://doi.org/10.1093/jn/138.5.833
  34. Tao, J. Y., Zhao, L., Huang, Z. J., Zhang, X. Y., Zhang, S. L.,Zhang, Q. G., Fei-Xiao, Zhang, B. H., Feng, Q. L. and Zheng, G. H. : Anti-inflammatory effects of ethanol extract from Kummerowia striata (Thunb.) Schindl on lps-stimulated RAW 264.7 cell. Inflammation. 31, 154 (2008). https://doi.org/10.1007/s10753-008-9061-7
  35. Zhao, L., Zhang, S. L., Tao, J. Y., Jin, F., Pang, R., Guo, Y. J., Ye, P., Dong, J. H. and Zheng, G. H. : Anti-inflammatory mechanism of a folk herbal medicine, Duchesnea indica (Andr) Focke at RAW264.7 cell line. Immunol. Invest. 37, 339 (2008). https://doi.org/10.1080/08820130802111589
  36. Jin, M., Suh, S. J., Yang, J. H., Lu, Y., Kim, S. J., Kwon, S., Jo, T. H., Kim, J. W., Park, Y. I., Ahn, G. W., Lee, C. K., Kim, C. H., Son, J. K., Son, K. H. and Chang, H. W. : Antiinflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-$\kappa B$ and ERK1/2 inactivation. Food Chem. Toxicol. 48, 3073 (2010). https://doi.org/10.1016/j.fct.2010.07.048
  37. Strieter, R. M., Kunkel, S. L. and Bone, R. C. : Role of tumor necrosis factor-$\alpha$ in disease states and inflammation. Crit. Care Med. 21, S447 (1993). https://doi.org/10.1097/00003246-199310001-00006
  38. Choi, E. M. and Koo, S. J. : Effects of dioscorea daemona roxb. stem extract on the inflammatory responses, antioxidant system and lipid levels in vivo and the production of inflammatory mediators in RAW264.7 cells. J. East. Asian Soc. Dietary Life 15, 707 (2005)
  39. Jung, D. H., Park, H. J., Byun, H. E., Park, Y. M., Kim, T. W., Kim, B. O., Um, S. H. and Pyo, S. : Diosgenin inhibits macrophage-derived inflammatory mediators through downregulation of CK2, JNK, NF-kappaB and AP-1 activation. Int. Immunopharmacol. 10, 1047 (2010). https://doi.org/10.1016/j.intimp.2010.06.004
  40. Hiransai, P., Ratanachaiyavong, S., Itharat, A., Graidist, P., Ruengrairatanaroj, P. and Purintrapiban, J. : Dioscorealide B suppresses LPS-induced nitric oxide production and inflammatory cytokine expression in RAW 264.7 macrophages: The inhibition of NF-kappaB and ERK1/2 activation. J. Cell. Biochem. 109, 1057 (2010) https://doi.org/10.1002/jcb.22535
  41. Rimbach, G., Park, Y. C., Guo, Q., Moini, H., Qureshi, N., Saliou, C., Takayama, K., Virgili, F. and Packer, L. : Nitric oxide synthesis and TNF-alpha secretion in RAW 264.7 macrophages: mode of action of a fermented papaya preparation. Life Sci. 67, 679 (2000). https://doi.org/10.1016/S0024-3205(00)00664-0