Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characteristics of immunomodulation by a Lactobacillus sakei proBio65 isolated from Kimchi

김치에서 분리한 Lactobacillus sakei proBio65의 면역 조절 특성

  • Lim, Jeong-Heui (Department of Applied Microbiology and Biotechnology, Yeungnam University) ;
  • Seo, Byoung-Joo (Department of Applied Microbiology and Biotechnology, Yeungnam University) ;
  • Kim, Jung-Eun (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Chae, Chang-Suk (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Im, Sin-Hyeog (Department of Pediatrics, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Hahn, Youn-Soo (Department of Pediatrics, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Park, Yong-Ha (Department of Applied Microbiology and Biotechnology, Yeungnam University)
  • Received : 2011.08.12
  • Accepted : 2011.09.06
  • Published : 2011.09.28
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Abstract

We isolated and identified a novel probiotic strain, Lactobacillus sakei proBio65 from Kimchi. To determine whether L. sakei proBio65 has an immunomodulatory effect, we investigated cells via an in vitro screening system which co-cultured freshly isolated mesenteric lymphocyte with probiotics. A significant increase of $Foxp3^+$ transcription regulatory factor expression was observed, followed by an increase in anti-inflammatory cytokines transcription regulatory factor. L. sakei proBio65 exhibited high levels of the IL-10/IL-12 production ratio and enhanced Foxp3 expression in vitro. L. sakei proBio65 may thus be therapeutically useful for the modulation of inflammatory immune disorders.

김치로부터 새로운 프로바이오틱균주인 Lactobacillus sakei proBio65를 분리하고 명명하였다. 형질전환 생쥐(Foxp3-GFP KI 생쥐)를 이용하여 L. sakei proBio65의 면역조절 메커니즘 규명 및 면역 조절능을 확인하고 in vivo 적용 질환제어 응용 가능성을 평가하였다. 조절 T 세포의 master 전사조절인자로 알려진 $Foxp3^+$를 선정하고, L. sakei가 $Foxp3^+$ 전사조절인자를 증가시키는지 확인하기 위해 확립된 세포기반 screening system을 이용하였다. 항 염증성 사이토카인 전사조질인자의 증가에 이어 $Foxp3^+$ 전자조절인자발현의 상당한 증가를 확인하였다. L. sakei proBio65는 염증성면역 장애의 조절에 치료적으로 유용할 것이다.

Keywords

References

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