Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Identification of Three Pseudomonas koreensis Strains with Anti-microbial Activities Producing Inducers of the Expression of Egr-1 Gene

Egr-1 유전자의 발현 유도물질을 생산하는 항균성 저 영양 세균의 분리 및 동정

  • Yoon, Sang-Hong (Dept. of Agricultural. Biotechnol., National Academy of Agricultural Science, RDA) ;
  • Kim, Dong-Gwan (Institute of Biotechnology, Vitrosys Inc.) ;
  • Lee, Young-Han (Dept. of Biomedical Science & Technology, Research Center for Transcription Control, Konkuk University) ;
  • Shin, Soon-Young (Dept. of Biomedical Science & Technology, Research Center for Transcription Control, Konkuk University) ;
  • Kwon, Soon-Woo (Dept. of Agricultural. Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Chang-Muk (Dept. of Agricultural. Biotechnol., National Academy of Agricultural Science, RDA) ;
  • Kang, Han-Chul (Dept. of Agricultural. Biotechnol., National Academy of Agricultural Science, RDA) ;
  • Koo, Bon-Sung (Dept. of Agricultural. Biotechnol., National Academy of Agricultural Science, RDA)
  • 윤상홍 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김동관 ((주)비트로시스 생명공학연구소) ;
  • 이영한 (건국대학교 의생명과학과) ;
  • 신순영 (건국대학교 의생명과학과) ;
  • 권순우 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이창묵 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 강한철 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 구본성 (농촌진흥청 국립농업과학원 농업생명자원부)
  • Received : 2011.04.21
  • Accepted : 2011.05.30
  • Published : 2011.06.28
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Abstract

The Egr-1 gene is known to be a transcription factor for activating the expression of many tumor-repressing genes. In this study, three strains activating the promoter of the Egr-1 gene were selected, through the use of Egr-1 luciferase reporter assay and western blotting, from amongst approximately 3,800 oligotrophic bacteria isolated from the cultivated soils of various regions within Korea. These strains were identified as Pseudomonas koreensis on the basis of phylogenetic tree analysis of their 16S ribosomal DNA sequences and biochemical characteristics analyses using a variety of commercial kits (API 20NE, ID 32GN, API ZYM kits). In addition, we discovered that these strains produced anti-bacterial activity against Bacillus subtilis, Staphylococcus aureus and Listeria monocytogenes.

전국 경작지 토양에서 분리한 저 영양 세균 3,800주를 대상으로 암 억제유전자의 작동과 관여하는 전사인자인 Egr-1의 promoter 활성화를 유도하는 세 균주(SSG5, SSG6, SSG10)가 Egr-1 reporter assay와 western blotting 분석으로 최종 선발되었다. 이들 선발 균주들은 16S rDNA와 상업용 생화학적 키트(API 20NE, API ZYM, ID 32GN) 분석에 의해 모두 Pseudomonas koreensis인 것으로 최종 동정되었다. 이들 균주들은 Egr-1 발현을 유도하는 물질뿐만 아니라 다양한 세균의(B. subtilis, S. aureus, and L. monocytogenes) 성장을 저해하는 항균물질도 생산하는 것을 확인하였다.

Keywords

References

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