Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Microbiological Purification of L-Arabitol from Xylitol Mother Liquor

  • Jiang, Mingguo (College of Chemical Engineering and Ecology, Guangxi University for Nationality) ;
  • Wang, Ben (College of Chemical Engineering and Ecology, Guangxi University for Nationality) ;
  • Yang, Lifang (College of Chemical Engineering and Ecology, Guangxi University for Nationality) ;
  • Lin, Shuangjun (Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University) ;
  • Cheng, Hairong (Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University)
  • Received : 2010.06.07
  • Accepted : 2010.09.16
  • Published : 2011.01.28
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Abstract

As a rare sugar alcohol, L-arabitol can be used in food and can prevent extra fat deposits in the intestinal tract. Commercially, L-arabitol is prepared from pure L-arabinose by hydrogenation, which needs a high temperature and high pressure, leading to a high production cost for Larabitol. Therefore, this study describes a novel L-arabitol production method based on biological purification from the xylitol mother liquor, a cheap and readily available raw material that contains a high concentration of Larabitol. First, a novel Bacillus megaterium strain was screened that can utilize xylitol, sorbitol, and mannitol, yet not L-arabitol. The isolated strain was inoculated into a medium containing the xylitol mother liquor under formulated culture conditions, where a high L-arabitol yield (95%) and high purity (80%) were obtained when the medium was supplemented with 50 g/l of xylitol mother liquor. Upon further purification of the fermentation broth by ion exchange and decolorization, L-arabitol was crystallized with a purity of 98.5%.

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References

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