Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Nitrifying Bacterial Community Structure of a Full-Scale Integrated Fixed-Film Activated Sludge Process as Investigated by Pyrosequencing

  • Kim, Taek-Seung (School of Civil, Environmental and Architectural Engineering, Korea University) ;
  • Kim, Han-Shin (School of Civil, Environmental and Architectural Engineering, Korea University) ;
  • Kwon, Soon-Dong (School of Civil, Environmental and Architectural Engineering, Korea University) ;
  • Park, Hee-Deung (School of Civil, Environmental and Architectural Engineering, Korea University)
  • Received : 2010.09.27
  • Accepted : 2010.12.01
  • Published : 2011.03.28
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Abstract

Nitrifying bacterial community structures of suspended and attached biomasses in a full-scale integrated fixed-film activated sludge process were investigated by analyzing 16S rRNA gene sequences obtained from pyrosequencing. The suspended biomass had a higher number of ammonia-oxidizing bacterial sequences (0.8% of total sequences) than the attached biomass (0.07%), although most of the sequences were within the Nitrosomonas oligotropha lineage in both biomasses. Nitrospira-like nitrite-oxidizing bacterial sequences were retrieved in the suspended biomass (0.06%), not in the attached biomass, whereas the existence of Nitrobacter-like sequences was not evident. The suspended biomass had higher nitrification activity (1.13 mg N/TSS/h) than the attached biomass (0.07 mg N/TSS/h). Overall, the results made it possible to conclude the importance of the suspended biomass, rather than the attached biomass, in nitrification in the wastewater treatment process studied.

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