Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Endocellulase-Free Multienzyme Complex from Newly Isolated Thermoanaerobacterium thermosaccharolyticum Strain NOI-1

  • Chimtong, Suphavadee (School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Tachaapaikoon, Chakrit (Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi) ;
  • Pason, Patthra (Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi) ;
  • Kyu, Khin Lay (School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Kosugi, Akihiko (Post-harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences) ;
  • Mori, Yutaka (Post-harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences) ;
  • Ratanakhanokchai, Khanok (School of Bioresources and Technology, King Mongkut's University of Technology Thonburi)
  • Received : 2010.09.27
  • Accepted : 2010.12.17
  • Published : 2011.03.28
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Abstract

An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were $60^{\circ}C$ and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, ${\beta}$-xylosidase, ${\alpha}$-L-arabinofuranosidase, ${\beta}$-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.

Keywords

References

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