Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

  • Gupta, Anil Kumar (Division of Comparative Genomics, Institute of Genomics and Integrative Biology) ;
  • Pathak, Rajiv (Division of Comparative Genomics, Institute of Genomics and Integrative Biology) ;
  • Singh, Bharat (Division of Comparative Genomics, Institute of Genomics and Integrative Biology) ;
  • Gautam, Hemlata (Division of Comparative Genomics, Institute of Genomics and Integrative Biology) ;
  • Kumar, Ram (Division of Comparative Genomics, Institute of Genomics and Integrative Biology) ;
  • Kumar, Raj (Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research and Development Organization, Ministry of Defence, Government of India) ;
  • Arora, Rajesh (Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research and Development Organization, Ministry of Defence, Government of India) ;
  • Gautam, Hemant K. (Division of Comparative Genomics, Institute of Genomics and Integrative Biology)
  • Received : 2010.12.27
  • Accepted : 2011.03.15
  • Published : 2011.06.28
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Abstract

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

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