Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mannitol Production by Leuconostoc citreum KACC 91348P Isolated from Kimchi

  • Otgonbayar, Gan-Erdene (Department of Food Science and Technology, Chungbuk National University) ;
  • Eom, Hyun-Ju (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Beom-Soo (Department of Chemical Engineering, Chungbuk National University) ;
  • Ko, Jae-Hyung (Department of Biotechnology and Food Science, Mongolia International University) ;
  • Han, Nam-Soo (Department of Food Science and Technology, Chungbuk National University)
  • Received : 2011.05.19
  • Accepted : 2011.06.09
  • Published : 2011.09.28
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Abstract

Leuconostoc genus, which comprise heterofermentative lactic acid bacteria, reduces fructose to mannitol by recycling intracellular NADH. To evaluate the mannitol productivities of different Leuconostoc species, 5 stock cultures and 4 newly isolated strains were cultivated in MRS and simplified media containing glucose and fructose (1:2 ratio). Among them, L. citreum KACC 91348P, which was isolated from kimchi, showed superior result in cell growth rate, mannitol production rate, and yield in both media. The optimal condition for mannitol production of this strain was pH 6.5 and $30^{\circ}C$. When L. citreum KACC was cultured in simplified medium in a 2 l batch fermenter under optimal conditions, the maximum volumetric productivity was 14.83 $g{\cdot}l^{-1}h^{-1}$ and overall yield was 86.6%. This strain is a novel and efficient mannitol producer originated from foods to be used for fermentation of fructose-containing foods.

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References

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