Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Profiling of Recovery Efficiencies for Three Standard Protocols (FDA-BAM, ISO-11290, and Modified USDA) on Temperature-Injured Listeria monocytogenes

  • Lee, Hai Yen (Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia) ;
  • Chai, Lay Ching (Department of Microbiology, Faculty of Science, Universiti Malaya) ;
  • Pui, Chai Fung (Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia) ;
  • Wong, Woan Chwen (Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia) ;
  • Mustafa, Shuhaimi (Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia) ;
  • Cheah, Yoke Kqueen (Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia) ;
  • Issa, Zuraini Mat (Faculty of Hotel and Tourism Management, Universiti Teknologi MARA Malaysia) ;
  • Nishibuchi, Mitsuaki (Center for Southeast Asian Studies, Kyoto University) ;
  • Radu, Son (Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia)
  • Received : 2010.12.28
  • Accepted : 2011.06.05
  • Published : 2011.09.28
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Abstract

There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures ($60^{\circ}C$ and $-20^{\circ}C$) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.

Keywords

References

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