Journal of the Institute of Electronics Engineers of Korea SC (전자공학회논문지SC)

The Institute of Electronics and Information Engineers (대한전자공학회)
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Development of DNA Sensor Using Magnetic Iron Oxide Nanoparticle

자성 산화철(iron oxide) 나노입자를 이용한 DNA 센서 개발

  • Nam, Ki-Chang (Clinical Trials Center for Medical Devices, Severance Hospital) ;
  • Song, Kwang-Soup (School of Electronic Engineering, Kumoh National Institute of Technology)
  • 남기창 (세브란스병원 의료기기 임상시험 센터) ;
  • 송광섭 (금오공과대학교 전자공학부)
  • Received : 2011.08.05
  • Published : 2011.11.25
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Abstract

The surface of magnetic iron oxide nanoparticles (${\gamma}-Fe_2O_3$) is functionalized ($-NH_2$, -COOH) with bifunctional organic molecules and evaluated using FT-IR (Fourier transform infrared spectroscopy). We immobilize 21-base pair probe DNA and hybridize fluorescence-labeled (Cy5) target DNA onto the functionalized iron oxide nanoparticles. The fluorescence images obtained from a confocal microscopy show that the functionalized iron oxide nanoparticles should detect the hybridization of complementary and noncomplementary DNA.

자성 산화철 나노입자(iron oxide nanoparticle, ${\gamma}-Fe_2O_3$) 표면을 기능성 유기 분자를 이용하여 아민기($-NH_2$), 카르복실기(-COOH)로 표면 처리 하였으며, 이들 기능기로 표면 처리된 산화철 나노입자를 FT-IR을 이용하여 나노입자 표면을 분석하였다. 아민기, 카르복실기로 표면처리된 산화철 나노입자 표면에 특정 배열을 갖는 21-base pair 길이의 프로브 DNA를 고정하였고, 형광 라벨(Cy5)이 부착된 상보적, 비상보적 타게트 DNA를 이용하여 고정된 프로브 DNA와 hybridization을 진행하였다. 각각의 상보적, 비상보적 타게트 DNA와 hybridization 처리한 산화철 나노입자를 confocal microscopy를 이용하여 관찰하였으며, 그 결과 산화철 나노입자를 이용하여 특정 배열의 DNA검출에 성공하였다.

Keywords

References

  1. 서화일, 손병기, "ISFET 바이오센서에의 적용을 위한 신호처리회로의 개발과 그들의 단일칩 집 적설계," 전자공학회논문지, 제28권 A편, 제1호 46-51쪽, 1991년 1월
  2. F. Uslu, S. Ingebrandt, D. Mayer, S. Bocker -Meffert, M. Odenthal and A. Offenhausser, "Labelfree fully electronic nucleic acid detection system based on a field-effect transistor device," Biosen. & Bioele., Vol. 19, no. 12, pp. 1723-1731, Jul. 2004. https://doi.org/10.1016/j.bios.2004.01.019
  3. 송광섭 "전계효과 트랜지스터(FETs)를 이용한 전 하 검출형 DNA 센서에서의 Debye length에 따른 검출 감도," 전자공학회논문지, 제48권 SC편, 제2 호 86-90쪽, 2011년 3월
  4. A. Poghossian, A. Cherstvy, S. Ingebrandt, A. Offenhausser and M. J. Schoning, "Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices," Sens. and Actu. B, Vol. 111-112, no. 11, pp. 470-480, Nov. 2005.
  5. K. S. Song, G. J. Zhang, Y. Nakamura, K. Furukawa, T. Hiraki, J. H. Yang, T. Funatsu, I. Ohodomari and H. Kawarada, "Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution," Physical Review E, Vol. 74, no. 4, pp. 041919-041925, Oct. 2006
  6. M. Su, S. Li and V. P. Dravid, "Microcantilever resonance-based DNA detection with nanoparticle probes," Appl. Phys. Lett. 82, no. 20, pp. 3562-3564, May 2003. https://doi.org/10.1063/1.1576915
  7. M. R. Nor, S. Goyal, S. M. Christensen and S. M. Iqbal, "Electrical detection of single-base DNA mutation using functionalized nanoparticles," Appl. Phys. Lett. 95, pp. 073703-073705, Aug. 2009. https://doi.org/10.1063/1.3152768
  8. Y. W. Jun, Y. M. Huh, J. S. Choi, J. H. Lee, H. T. Song, S. Kim, S. Yoon, K. S. Kim, J. S. Shin, J. S. Suh and J. Cheon, "Nanoscale Size Effect of Magnetic Nanocrystals and Their Utilization for Cancer Diagnosis via Magnetic Resonance Imaging," J. Am. Chem. Soc. 127, pp. 5732-5733, Feb. 2005. https://doi.org/10.1021/ja0422155
  9. U. Hafeli, W. Schutt, J. Teller and M. Zborowski, "Scientific and clinical applications of magnetic carriers," Springer Press 1st edition 1997.
  10. I. Koh, X. Wang, B. Varughese, L. Isaacs, S. H. Ehrman and D. S. English, "Magnetic Iron Oxide Nanoparticles for Biorecognition: Evaluation of Surface Coverage and Activity," J. Phys. Chem. B 110, pp. 1553-1558, Dec. 2005.
  11. Y. Lu, Y. Yin, B. T. Mayers and Y. Xia, "Modifying the surface properties of superparamagnetic iron oxide nanoparticles through A sol.gel approach," Nano Lett. 2, no. 3, pp. 183-186, Jan. 2002. https://doi.org/10.1021/nl015681q
  12. J. H. Yang, K. S. Song, G. J. Zhang, M. Degawa, Y. Sasaki, I. Ohdomari and H. Kawarada, "Characterization of DNA hybridization on partially aminated diamond by aromatic compounds," Langmuir, 22 pp. 11245-11250, Jul. 2006. https://doi.org/10.1021/la060677l