Changes of Sex Hormones and Cervical Mucus in Estrus-synchronized Himalayan Tahrs (Hemitragus jemlahicus) in Non-breeding Season

  • Received : 2011.08.07
  • Accepted : 2011.08.30
  • Published : 2011.09.30

Abstract

Eight female Himalayan tahrs (Hemitragus jemlahicus) were estrus-synchronized, and transcervically inseminated with frozen-thawed semen in September, 2009, about 2 to 3 months earlier than their natural breeding season. Intravaginal progesterone-releasing devices were inserted into vaginas of six Himalayan tahrs on September 7, and the other two on September 8 to suppress luteal function of ovaries. The devices had been placed deep inside the vagina prior to withdrawal on September 23. A day before CIDR removal, a combination of PMSG 400 IU and hCG 200 IU was intramuscularly injected. Forty hours later, frozen-thawed semen was transcervically inseminated. Pregnancy diagnosis was performed 39 days later by analyzing progesterone level of serum. Every treatment was done under anesthesia inducted by xylazine injection. In conclusion, vaginal discharge of cervical mucus, hormonal changes induced by implant-typed or muscularly injectable hormones and widening of cervix enough to insert an insemination gun into uterine body were achieved in non-breeding season. Moreover, the first inseminated Himalayan tahr, 36 hours after CIDR removal was assumed to be pregnant but the fetus may have been lost due to the use of anesthetic drug.

Keywords

References

  1. Asher GW, Fisher MW, Fennessy PF, Mackintosh CG, Jabbour H and Morrow CJ. 1993. Oestrus synchronization, semen collection and artificial insemination of farmed red deer (Cervus elephus) and fallow deer (Dama dama). Anim. Reprod. Sci. 33: 241-265. https://doi.org/10.1016/0378-4320(93)90118-B
  2. Dematteis A, Menzano A, Tizzani P, Karmacharya B, Meneguz PG and Lovari S. 2006. Immobilization of Himalayan tahr with a xylazine-ketamine mixture and reversal with atipamezole under field conditions. J. Wildl. Dis. 42:633 -639. https://doi.org/10.7589/0090-3558-42.3.633
  3. Garde JJ, Soler AJ, Cassinello J, Crespo C, Malo AF, Espeso G, Gomendio M and Roldan ERS. 2003. Sperm cryopreservation in three species of endangered gazelles. Biol. Re prod. 69:602- 611. https://doi.org/10.1095/biolreprod.102.012914
  4. Holtz WV, Moore HDM, North RD, Hartman TD and Hodges JK. 1988. Hormonal and behavioural detection of oestrus in black buck, Antilope cervicapra, and successful artificial insemination with fresh and frozen semen. J. Reprod. Fert. 82:717-725. https://doi.org/10.1530/jrf.0.0820717
  5. Johnston SD, Blyde D, Pedrana R and Gibbs A. 2000. Laparoscopic intrauterine insemination in Barbary sheep (Ammotragus lervia). Aust. Vet. J. 78:714-717. https://doi.org/10.1111/j.1751-0813.2000.tb10414.x
  6. Keskintepe L, Simplicio AA and Brackett BG. 1998. Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders. Theriogenology 49: 1265-1274. https://doi.org/10.1016/S0093-691X(98)00074-0
  7. Mejia M, Medrano A, Gonzalez-Rebeles C and Mejia O. 2009. Capacitation status of frozen-thawed spermatozoa from wild ruminant species. Eur. J. Wildl. Res. 55:1-6. https://doi.org/10.1007/s10344-008-0207-6
  8. Purdy PH. 2006. A review on goat sperm cryopreservation. Small. Rum. Res. 63:215-225. https://doi.org/10.1016/j.smallrumres.2005.02.015
  9. Sakamoto H, Misumi K, Nakama M and Aoki Y. 1996. The effects of xylazine on intrauterine pressure, uterine blood flow, maternal and fetal cardiovascular and pulmonary function in pregnant goats. J. Vet. Med. Sci. 58:211-217. https://doi.org/10.1292/jvms.58.211
  10. Sohnrey B and Holtz W. 2005. Technical note: Transcervical deep corneal insemination of goats. J. Anim. Sci. 83:1543- 1548. https://doi.org/10.2527/2005.8371543x
  11. Watson PF. 1976. Electroejaculation, semen characteristics and semen preservation of the brindled gnu. J. Reprod. Fert. 47: 123-126. https://doi.org/10.1530/jrf.0.0470123
  12. Yong HY, Park JE, Kim MA, Bae BS, Kim SD, Ha YH, Oh CS, Kim DH, Kim MH, Yoo MH, Jeong YJ and Ro SC. 2010. Transcervical or laparoscopic insemination of frozenthawed semen in estrus-synchronized Himalayan tahrs (Hemitragus jemlahicus). J. Emb. Trans. 25:291-295.