Reproductive and Developmental Biology

  • 제35권3호
  • /
  • Pages.363-367
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  • 2011
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

자기활성수가 소 정액 동결에 미치는 영향

Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa

  • 서기범 (강원대학교 동물생명과학대학) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 이경진 (강원대학교 동물생명과학대학) ;
  • 유한준 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학과) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 이승환 (국립축산과학원 한우시험장) ;
  • 이진우 ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Seo, Gi-Beom (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Yu, Han-Jun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seung-Hwan (National Institute of Animal Science, Hanwoo Ex) ;
  • Lee, Jin-Woo (ROHAS TECH) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 투고 : 2011.09.07
  • 심사 : 2011.09.19
  • 발행 : 2011.09.30
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초록

The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl$^{(R)}$ solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for S min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.

키워드

참고문헌

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