Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Effects of Gamma Irradiation on the Survival and Development of the Multicolored Asian Ladybird Beetle, Harmonia axyridis (Coccinellidae; Coleoptera)

감마선이 무당벌레의 생존과 발육에 미치는 영향

  • Seo, Mi-Ja (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Lee, Hee-Jin (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kang, Eun-Jin (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kang, Si-Yong (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Yu, Yong-Man (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Youn, Young-Nam (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University)
  • 서미자 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이희진 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 강은진 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 강시용 (한국원자력연구원 정읍방사선과학연구소) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2011.10.04
  • Accepted : 2011.11.10
  • Published : 2011.12.30
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Abstract

The effects of gamma irradiation on the survival, development, fecundity, and sex ratio of the multicolored Asian ladybird beetle (Harmonia axyridis) were investigated. A dose of more than 50 Gy dose of irradiation on eggs completely inhibited hatch, and 1st and 3rd instar larva irradiated with over 40 Gy almost died. Adverse effects of gamma irradiation over 70 Gy were also observed in the survival and development of pupae and adults. Although irradiated individuals usually survived, they had longer periods of larval and pupal development than untreated individuals. Slight female-biased sex ratios were observed in some treatments. However, sex ratio differences soon equilibrated. Generally, inherited harmful effects of gamma irradiation were observed in F1 and F2 generations. Comet tail indicating DNA damage was showed in survival individuals exposed to gamma irradiation.

감마선을 조사하였을 경우에 무당벌레의 생존 및 발육, 산란, 부화와 같은 생식작용 및 이들 자손들의 성비에 미치는 영향을 조사하였다. 무당벌레 알은 조사선량 50 Gy 이상에서는 부화하지 못했으며, 1령과 3령 유충은 40 Gy 이상에서는 대부분 사망하였다. 번데기와 성충도 70 Gy 이상의 감마선 조사선량에 대해 조금씩 영향을 받기 시작하였다. 일정량의 감마선에 노출된 후에 정상적으로 생존하였다 하더라도, 유충과 번데기의 발육기간이 정상개체에 비해 길어지는 경향을 나타내었다. 감마선에 노출된 성충의 자손들은 일부 처리구에서 암컷이 편중적으로 나타나기도 하였으나, 세대가 진행될수록 성비가 안정되어 가는 것도 확인되었다. 대체적으로 F1과 F2세대에서 감마선에 의해 영향을 받는 것으로 확인되었다. 감마선에 조사된 후 생존한 개체의 세포를 코멧분석한 결과, DNA손상에 의한 코멧꼬리를 확인할 수 있었다.

Keywords

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