Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Hop stunt viroid (HSVd) Sequence Variants from Dapple Fruits of Plum (Prunus salicina L.) in Korea

국내 얼룩반점 자두에서 발생한 호프왜화바이로이드(HSVd)의 유전자 변이

  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Chung, Bong-Nam (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Jeom-Doeg (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug-Seoun (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Jeong-Soo (Crop Protection Division, National Academy of Agricultural Science)
  • 조인숙 (국립원예특작과학원 원예특작환경과) ;
  • 정봉남 (국립원예특작과학원 원예특작환경과) ;
  • 조점덕 (국립원예특작과학원 원예특작환경과) ;
  • 최승국 (국립원예특작과학원 원예특작환경과) ;
  • 최국선 (국립원예특작과학원 원예특작환경과) ;
  • 김정수 (국립농업과학원 작물보호과)
  • Received : 2011.10.14
  • Accepted : 2011.11.18
  • Published : 2011.12.31
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Abstract

Dapple fruits of plum cv. Oiishiwase (Prunus salicina L.) were occurred at Gyeonggi-do and Gyeongsangbukdo. The symptoms resembled the dapple fruit disease caused by Hop stunt viroid (HSVd). To identify the causal disease agents, RT-PCR was performed with the specific primers of HSVd. RT-PCR analysis showed that HSVd variants (DP1, DP2) were detected from dapple fruits. HSVd detection was also confirmed by the dot blot hybridization using a DIG-probe specific to HSVd. Nucleotide sequences of DP1 and DP2 had the identities of 94-100% with those of other 7 variants of HSVd in Genbank database. DP1 and DP2 were different in two nucleotides of CG and AA at position of 59 and 60, orderly. Based on nucleotide sequences at position of 59 and 60, HSVd variants associated with plum dapple fruits could be divided mainly into three groups as CG, AA and TG.

경기도와 경상북도 일대의 '대석조생' 자두 재배포장에서 착색이 불균일한 얼룩 반점 증상의 과실이 발생하였다. 이들 시료로부터 유전자 진단(RT-PCR)과 Dot-blot hybridization 검정 결과 호프왜화바이로이드(HSVd)가 검출되었고 유전자 염기서열 분석을 통해 2개의 HSVd 변이주(DP1, DP2) 를 동정하였다. GenBank에 등록된 자두 HSVd의 7개 변이주와 새로운 DP1, DP2 변이주와의 유전자 염기서열을 비교분석한 결과 94-100%의 높은 상동성을 보였다. DP1와 DP2의 유전자는 59번과 60번 염기가 각각 CG, AA로 서로 다른 변이를 보였다. 국내외 자두 과실 얼룩반점 증상에서 동정된 HSVd의 유전자는 특이적으로 59번과 60번 부위의 염기가 CG, AA, TG인 3개 군으로 크게 구분되는 것을 알 수 있었다.

Keywords

References

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