Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Phytoplasmal Disease Occurred on Floricultural Crops in Korea

우리나라 화훼류 파이토플라스마병의 특성

  • Chung, Bong-Nam (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jeong, Myeong-Il (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug-Sun (National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Received : 2011.03.18
  • Accepted : 2011.10.28
  • Published : 2011.12.31
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Abstract

Seven phytoplasma diseases have been occurred on floricultural crops in Korea : Ph-ch1 and Ph-ch2 of chrysanthemum, Ph-lily of lily, petunia flat stem-Korean (PFS-K) of petunia, poinsettia branch inducing- Korean (PoiBI-K) of poinsettia, statis witches' broom-Korean (SWB-K) of statis and azalea witches broom (AWB). Classification of the seven phytoplasmal diseases based on 16S ribosomal RNA (rRNA) sequences showed that floricultural crop phytoplasma disease were widespread in order of aster yellow (AY), stolbur and X-disease in Korea. In phenotypic characters, the fasciation was occurred in both monocotyledon plant of lily and dicotyledon plants of petunia and poinsettia. Besides, the fascination was occurred in Ph-lily of stolbur, petunia PFS-K of AY and PoiBI-K of X-disease. This result indicated that phytoplasma classification based on 16S rRNA and symptoms are not consistently related. The comparison of 16S rRNA sequence of the seven floricultural crop phytoplasma with five tree phytoplasmal diseases of jujube witches' broom, paulownia witches' broom, wild jujube witches' broom, mulberry dwarf, golden rain phytoplasma occurred in Korea showed as high as 88.5-99.9% homology. Among them, especially mulberry dwarf showed the highest homology with the seven floricultural crop phytoplasms. Based on this result, floricultural crop phytoplasmas were assumed to be transmitted by insect vectors from tree phytoplasmas in Korea.

우리나라에서 화훼류에 7종류의 파이토플라스마병이 발생하였다. 국화의 Ph-ch1과 Ph-ch2, 나리의 Ph-lily, 페튜니아의 petunia flat stem(PFS-K), 포인세티아의 poinsettia branch inducing(PoiBI-K), 스타티스의 statis witches' broom (SWB-K)과 아잘레아의 azalea witches broom(AWB) 등이다. 16S rRNA 유전자 염기서열을 기본으로 화훼류 파이토플라스마를 분류한 결과 우리나라에는 aster yellow(AY), stolbur와 X-disease 순으로 많이 발생하였다. 파이토플라스마의 특징적인 병징 가운데 하나인 대화증상은 단자엽 식물인 나리와 페튜니아, 포인세티아와 같은 쌍자엽식물에서 모두 발생하였다. 또한 대화증상은 stolbur 그룹의 Ph-lily, AY 그룹의 petunia PFS-K와 X-disease의 포인세티아 PoiBI-K에서 모두 나타났다. 이 결과는 16S rRNA 유전자 염기서열에 기초를 둔 파이토플라스마 분류와 증상과는 일관성있게 일치하지 않는다는 것을 알 수 있다. 우리나라 화훼류에서 발생한 7종의 파이토플라스마를 대추나무빗자루, 오동나무빗자루, 묏대추나무빗자루, 뽕나무 오갈 및 모감주나무파이토플라스마 등 5종의 수목 파이 토플라스마와 16S rRNA 유전자의 염기서열을 비교한 결과 88.5-99.9%의 매우 높은 상동성을 나타내었다. 특히 뽕나무오갈병 파이토플라스마는 PoiBI-K를 제외한 6종의 화훼류 파이토플라스마와 96.3-99.9% 가장 높은 상동성을 나타내었다. 이 결과로 우리나라 화훼류에 발생한 파이토플라스마병은 매개충을 통하여 수목으로부터 전염되었을 것으로 추정되었다.

Keywords

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